APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:500-1:3000 |
1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
HepG2 , NIH-3T3
Predict Reactivity
Rat, Zebrafish, Rabbit, Sheep, Bovine, Dog, Chicken, Chimpanzee(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS, 10% Glycerol
Preservative
0.01% Thimerosal
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.15 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human GDI1. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_1240886
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
GDP dissociation inhibitor 1 , 1A , GDIL , MRX41 , MRX48 , OPHN2 , RABGD1A , RABGDIA , XAP-4
Cellular Localization
Cytoplasm
Background
GDP dissociation inhibitors are proteins that regulate the GDP-GTP exchange reaction of members of the rab family, small GTP-binding proteins of the ras superfamily, that are involved in vesicular trafficking of molecules between cellular organelles. GDIs slow the rate of dissociation of GDP from rab proteins and release GDP from membrane-bound rabs. GDI1 is expressed primarily in neural and sensory tissues. Mutations in GDI1 have been linked to X-linked nonspecific mental retardation. [provided by RefSeq]
Database
Research Area
DATA IMAGES
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GTX101731 IHC-P Image
GDI1 antibody detects GDI1 protein at cytosol on mouse lung by immunohistochemical analysis. Sample: Paraffin-embedded mouse lung. GDI1 antibody (GTX101731) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX101731 IHC-P Image
GDI1 antibody detects GDI1 protein at cytosol on mouse kidney by immunohistochemical analysis. Sample: Paraffin-embedded mouse kidney. GDI1 antibody (GTX101731) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX101731 WB Image
Sample (30 ug of whole cell lysate) A:NIH-3T3 10% SDS PAGE GTX101731 diluted at 1:1000
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GTX101731 IHC-P Image
GDI1 antibody detects GDI1 protein at cytosol on U87 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded U87 xenograft. GDI1 antibody (GTX101731) dilution: 1:200.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX101731 WB Image
Sample (30 ug of whole cell lysate) A: Hep G2 (GTX27900) 10% SDS PAGE GTX101731 diluted at 1:1000
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REFERENCE
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REVIEW
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