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GDNF antibody [12F9]

Anti-GDNF antibody [12F9] used in Western Blot (WB). GTX52761

Cat. No. GTX52761

Reference 1

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 12F9

Isotype

 IgG2

Application

 WB, Functional Assay, Neutralizing/Inhibition

Reactivity

 Human
Package
100 μg ($399)
See all GDNF products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:1000
Functional Assay Assay dependent
Neutralizing/Inhibition Assay dependent
Not tested in other applications.

Calculated MW

24 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

500 μg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Human recombinant GDNF

Purification

Protein G purified
From tissue culture supernatant

Conjugation

Unconjugated

RRID

AB_2887807

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ATF , ATF1 , ATF2 , GDNF , HFB1GDNF , HSCR3 , glial cell derived neurotrophic factor

Cellular Localization

Secreted

Background

This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate each subunit of the disulfide-linked homodimer. The recombinant form of this protein, a highly conserved neurotrophic factor, was shown to promote the survival and differentiation of dopaminergic neurons in culture, and was able to prevent apoptosis of motor neurons induced by axotomy. This protein is a ligand for the product of the RET (rearranged during transfection) protooncogene. Mutations in this gene may be associated with Hirschsprung disease and Tourette syndrome. This gene encodes multiple protein isoforms that may undergo similar proteolytic processing. [provided by RefSeq, Aug 2016]

Database

Research Area

DATA IMAGES

Anti-GDNF antibody [12F9] used in Western Blot (WB). GTX52761

GTX52761 WB Image

WB analysis of human placenta tissue lysate using GTX52761 GDNF antibody [12F9].

REFERENCE

Application Reference

Shvartsman D et al. Mol Ther 2014; Sustained Delivery of VEGF Maintains Innervation and Promotes Reperfusion in Ischemic Skeletal Muscles Via NGF/GDNF Signaling.
Application : Functional Assay
Reactivity : Human
Submit a Reference

REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 399