APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
Assay dependent |
Assay dependent |
Assay dependent |
Assay dependent |
Note :
WB
A non-reduced and/or reduced sample treatment and 6-15 % gradient SDS-PAGE was used.
IHC-Fr
Cells were either fixed with methanol at -20 °C or with 3% PFA followed by permeabilization with Triton X-100.
|
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
Filter-sterilized PBS, 0.1% BSA
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.
Concentration
100 μg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Dog
Immunogen
Protein fraction isolated from detergent extracts of MDCK cells1.
Purification
Purified IgG1
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
ARHGEF2,GEF,GEFH1,LFP40,P40,Rho/Rac guanine nucleotide exchange factor 2,GEF-H1
Cellular Localization
Cytoplasm,Cytoskeleton,Cell junction,Tight junction,Golgi apparatus,Spindle,Cell projection,Ruffle membrane,Cytoplasmic vesicle
Background
Rho GTPases play a fundamental role in numerous cellular processes that are initiated by extracellular stimuli that work through G protein coupled receptors. The encoded protein may form complex with G proteins and stimulate rho-dependent signals. Alternatively spliced transcript variants encoding different isoforms have been identified.[provided by RefSeq, Jun 2009]
Database
Research Area
REFERENCE
There are currently no references for GEF-H1 antibody [B4/7] (GTX54457). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for GEF-H1 antibody [B4/7] (GTX54457). Be the first to share your experience with this product.