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GEF-H1 antibody [B4/7]

Cat No. GTX54457

Host

Mouse

Clonality

Monoclonal

Clone Name

B4/7

Isotype

IgG1

Application

WB, ICC/IF, IHC-Fr, IP

Reactivity

Human, Dog
Package
50 μg ($319)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB Assay dependent
ICC/IF Assay dependent
IHC-Fr Assay dependent
IP Assay dependent

Note :

WB
A non-reduced and/or reduced sample treatment and 6-15 % gradient SDS-PAGE was used.

IHC-Fr
Cells were either fixed with methanol at -20 °C or with 3% PFA followed by permeabilization with Triton X-100.

Not tested in other applications.

Calculated MW

112 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

0.2 μm filtered in PBS, 0.1% BSA, 0.02% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.

Concentration

100 μg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Dog

Immunogen

Protein fraction isolated from detergent extracts of MDCK cells1.

Purification

Purified IgG1

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

ARHGEF2,GEF,GEFH1,LFP40,P40,Rho/Rac guanine nucleotide exchange factor 2,GEF-H1

Cellular Localization

Cytoplasm,Cytoskeleton,Cell junction,Tight junction,Golgi apparatus,Spindle,Cell projection,Ruffle membrane,Cytoplasmic vesicle

Background

Rho GTPases play a fundamental role in numerous cellular processes that are initiated by extracellular stimuli that work through G protein coupled receptors. The encoded protein may form complex with G proteins and stimulate rho-dependent signals. Alternatively spliced transcript variants encoding different isoforms have been identified.[provided by RefSeq, Jun 2009]

Database

Research Area

REFERENCE

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REVIEW

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Package List Price ($)
$ 319