GEF-H1 antibody [GT1132]

Name: GEF-H1 antibody [GT1132]
Brand: GeneTex
SKU: GTX629399
Price: 399.00 USD
Rating: 5 (1 reviews)

Anti-GEF-H1 antibody [GT1132] used in Western Blot (WB). GTX629399

Anti-GEF-H1 antibody [GT1132] used in IHC (Paraffin sections) (IHC-P). GTX629399

Cat. No. GTX629399

Host	Mouse
Clonality	Monoclonal
Clone Name	GT1132
Isotype	IgG2b
Application	WB, IHC-P
Reactivity	Human, Mouse, Rat
Package	100 μl ($399), 25 μl ($169)

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Datasheet
Document

View product citations for antibody GTX629399 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
IHC-P	1:100-1:1000

Not tested in other applications.

Calculated MW

112 kDa. ( Note )

Positive Control

293T , A431 , HeLa , Neuro2A , GL261 , C8D30 , NIH-3T3 , BCL-1 , Raw264.7 , C2C12 , PC-12 , Rat-2

Predict Reactivity

Bovine, Dog, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human GEF-H1. The exact sequence is proprietary.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_2888105

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Rho/Rac guanine nucleotide exchange factor 2 , GEF , GEF-H1 , GEFH1 , LFP40 , NEDMHM , P40

Cellular Localization

Cytoplasm , Cell junction , tight junction , Golgi apparatus , Spindle

Background

Rho GTPases play a fundamental role in numerous cellular processes that are initiated by extracellular stimuli that work through G protein coupled receptors. The encoded protein may form complex with G proteins and stimulate rho-dependent signals. [provided by RefSeq]

Database

Gene ID: 9181 ARHGEF2
UniProt: Q92974 ARHGEF2

Research Area

DATA IMAGES

GTX629399 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GEF-H1 antibody [GT1132] (GTX629399) diluted at 1:1000. The HRP-conjugated anti-mouset IgG antibody (GTX213111-01) was used to detect the primary antibody.

GTX629399 IHC-P Image

GEF-H1 antibody [GT1132] detects GEF-H1 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
GEF-H1 stained by GEF-H1 antibody [GT1132] (GTX629399) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX629399 IHC-P Image

GEF-H1 antibody [GT1132] detects GEF-H1 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
GEF-H1 stained by GEF-H1 antibody [GT1132] (GTX629399) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX629399 WB Image

GEF-H1 antibody [GT1132] detects GEF-H1 protein by Western blot analysis.
A. 30 μg PC-12 whole cell lysate/extract
B. 30 μg Rat-2 whole cell lysate/extract
5 % SDS-PAGE
GEF-H1 antibody [GT1132] (GTX629399) dilution: 1:1000

GTX629399 WB Image

GEF-H1 antibody [GT1132] detects GEF-H1 protein by Western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg A431 whole cell lysate/extract
C. 30 μg HeLa whole cell lysate/extract
5 % SDS-PAGE
GEF-H1 antibody [GT1132] (GTX629399) dilution: 1:1000

GTX629399 WB Image

GEF-H1 antibody [GT1132] detects GEF-H1 protein by Western blot analysis.
A. 30 μg Neuro2A whole cell lysate/extract
B. 30 μg GL261 whole cell lysate/extract
C. 30 μg C8D30 whole cell lysate/extract
D. 30 μg NIH-3T3 whole cell lysate/extract
E. 30 μg BCL-1 whole cell lysate/extract
F. 30 μg Raw264.7 whole cell lysate/extract
G. 30 μg C2C12 whole cell lysate/extract
5 % SDS-PAGE
GEF-H1 antibody [GT1132] (GTX629399) dilution: 1:1000

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