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GEF-H1 antibody [GT1132]

Anti-GEF-H1 antibody [GT1132] used in Western Blot (WB). GTX629399
Anti-GEF-H1 antibody [GT1132] used in Western Blot (WB). GTX629399
Anti-GEF-H1 antibody [GT1132] used in IHC (Paraffin sections) (IHC-P). GTX629399
Anti-GEF-H1 antibody [GT1132] used in IHC (Paraffin sections) (IHC-P). GTX629399
Anti-GEF-H1 antibody [GT1132] used in Western Blot (WB). GTX629399
Anti-GEF-H1 antibody [GT1132] used in Western Blot (WB). GTX629399

Cat No. GTX629399

Host

Mouse

Clonality

Monoclonal

Clone Name

GT1132

Isotype

IgG2b

Application

WB, IHC-P

Reactivity

Human, Mouse, Rat
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

112 kDa. ( Note )

Positive Control

293T , A431 , HeLa , Neuro2A , GL261 , C8D30 , NIH-3T3 , BCL-1 , Raw264.7 , C2C12 , PC-12 , Rat-2 , *3A6

Predict Reactivity

Bovine, Dog, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human GEF-H1. The exact sequence is proprietary.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_2888105

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Rho/Rac guanine nucleotide exchange factor 2 , GEF , GEF-H1 , GEFH1 , LFP40 , NEDMHM , P40

Cellular Localization

Cytoplasm , Cell junction , tight junction , Golgi apparatus , Spindle

Background

Rho GTPases play a fundamental role in numerous cellular processes that are initiated by extracellular stimuli that work through G protein coupled receptors. The encoded protein may form complex with G proteins and stimulate rho-dependent signals. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-GEF-H1 antibody [GT1132] used in Western Blot (WB). GTX629399

GTX629399 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GEF-H1 antibody [GT1132] (GTX629399) diluted at 1:1000. The HRP-conjugated anti-mouset IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-GEF-H1 antibody [GT1132] used in Western Blot (WB). GTX629399

GTX629399 WB Image

GEF-H1 antibody [GT1132] detects GEF-H1 protein by Western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg A431 whole cell lysate/extract
C. 30 μg HeLa whole cell lysate/extract
5 % SDS-PAGE
GEF-H1 antibody [GT1132] (GTX629399) dilution: 1:1000

Anti-GEF-H1 antibody [GT1132] used in IHC (Paraffin sections) (IHC-P). GTX629399

GTX629399 IHC-P Image

GEF-H1 antibody [GT1132] detects GEF-H1 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
GEF-H1 stained by GEF-H1 antibody [GT1132] (GTX629399) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-GEF-H1 antibody [GT1132] used in IHC (Paraffin sections) (IHC-P). GTX629399

GTX629399 IHC-P Image

GEF-H1 antibody [GT1132] detects GEF-H1 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
GEF-H1 stained by GEF-H1 antibody [GT1132] (GTX629399) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-GEF-H1 antibody [GT1132] used in Western Blot (WB). GTX629399

GTX629399 WB Image

GEF-H1 antibody [GT1132] detects GEF-H1 protein by Western blot analysis.
A. 30 μg PC-12 whole cell lysate/extract
B. 30 μg Rat-2 whole cell lysate/extract
5 % SDS-PAGE
GEF-H1 antibody [GT1132] (GTX629399) dilution: 1:1000

Anti-GEF-H1 antibody [GT1132] used in Western Blot (WB). GTX629399

GTX629399 WB Image

GEF-H1 antibody [GT1132] detects GEF-H1 protein by Western blot analysis.
A. 30 μg Neuro2A whole cell lysate/extract
B. 30 μg GL261 whole cell lysate/extract
C. 30 μg C8D30 whole cell lysate/extract
D. 30 μg NIH-3T3 whole cell lysate/extract
E. 30 μg BCL-1 whole cell lysate/extract
F. 30 μg Raw264.7 whole cell lysate/extract
G. 30 μg C2C12 whole cell lysate/extract
5 % SDS-PAGE
GEF-H1 antibody [GT1132] (GTX629399) dilution: 1:1000

REFERENCE

There are currently no references for GEF-H1 antibody [GT1132] (GTX629399). Be the first to share your publications with this product.

REVIEW

GEF-H1 antibody [GT1132] Cat No. GTX629399

Rating ( Average 5 based on 1 users reviews)
Application
Western Blot(WB) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 12-Jun-2015
Score :
Application Tested : WB
Sample Species : Hu
Sample : 3A6 cells
Amount used : 30μg
Blocking : 3% BSA in TBST, 25°C, 1Hr
Primary Antibody : 1:2000, 4°C, 16Hr
SDS
PBS.pdf
Package List Price ($)
$ 329
$ 169