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GEF-H1 antibody [GT336]

Anti-GEF-H1 antibody [GT336] used in Western Blot (WB). GTX629398
Anti-GEF-H1 antibody [GT336] used in Western Blot (WB). GTX629398
Anti-GEF-H1 antibody [GT336] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX629398
Anti-GEF-H1 antibody [GT336] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX629398
Anti-GEF-H1 antibody [GT336] used in Western Blot (WB). GTX629398
Anti-GEF-H1 antibody [GT336] used in Western Blot (WB). GTX629398

Cat No. GTX629398

Host

Mouse

Clonality

Monoclonal

Clone Name

GT336

Isotype

IgG1

Application

WB, ICC/IF

Reactivity

Human, Mouse, Rat
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:50-1:1000
Not tested in other applications.

Calculated MW

112 kDa. ( Note )

Positive Control

293T , A431 , HeLa , C8D30 , BCL-1 , Raw264.7 , C2C12 , Rat-2 , *3A6

Predict Reactivity

Bovine, Dog, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human GEF-H1. The exact sequence is proprietary.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_2888104

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Rho/Rac guanine nucleotide exchange factor 2 , GEF , GEF-H1 , GEFH1 , LFP40 , NEDMHM , P40

Cellular Localization

Cytoplasm , Cell junction , tight junction , Golgi apparatus , Spindle

Background

Rho GTPases play a fundamental role in numerous cellular processes that are initiated by extracellular stimuli that work through G protein coupled receptors. The encoded protein may form complex with G proteins and stimulate rho-dependent signals. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-GEF-H1 antibody [GT336] used in Western Blot (WB). GTX629398

GTX629398 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GEF-H1 antibody [GT336] (GTX629398) diluted at 1:500. The HRP-conjugated anti-mouset IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-GEF-H1 antibody [GT336] used in Western Blot (WB). GTX629398

GTX629398 WB Image

GEF-H1 antibody [GT336] detects GEF-H1 protein by Western blot analysis.
A. 30 μg Rat-2 whole cell lysate/extract
5 % SDS-PAGE
GEF-H1 antibody [GT336] (GTX629398) dilution: 1:500

Anti-GEF-H1 antibody [GT336] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX629398

GTX629398 ICC/IF Image

GEF-H1 antibody [GT336] detects GEF-H1 protein at cytoskeleton by immunofluorescent analysis.
Sample: MDA-MB-231 cells were fixed in methanol for 5 min.
Green: GEF-H1 protein stained by GEF-H1 antibody [GT336] (GTX629398) diluted at 1:50.

Anti-GEF-H1 antibody [GT336] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX629398

GTX629398 ICC/IF Image

GEF-H1 antibody [GT336] detects GEF-H1 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at room temperature.
Green: GEF-H1 protein stained by GEF-H1 antibody [GT336] (GTX629398) diluted at 1:50.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-GEF-H1 antibody [GT336] used in Western Blot (WB). GTX629398

GTX629398 WB Image

GEF-H1 antibody [GT336] detects GEF-H1 protein by Western blot analysis.
A. 30 μg C8D30 whole cell lysate/extract
B. 30 μg BCL-1 whole cell lysate/extract
C. 30 μg Raw264.7 whole cell lysate/extract
D. 30 μg C2C12 whole cell lysate/extract
5 % SDS-PAGE
GEF-H1 antibody [GT336] (GTX629398) dilution: 1:500

Anti-GEF-H1 antibody [GT336] used in Western Blot (WB). GTX629398

GTX629398 WB Image

GEF-H1 antibody [GT336] detects GEF-H1 protein by Western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg A431 whole cell lysate/extract
C. 30 μg HeLa whole cell lysate/extract
7.5 % SDS-PAGE
GEF-H1 antibody [GT336] (GTX629398) dilution: 1:500

REFERENCE

There are currently no references for GEF-H1 antibody [GT336] (GTX629398). Be the first to share your publications with this product.

REVIEW

GEF-H1 antibody [GT336] Cat No. GTX629398

Rating ( Average 5 based on 1 users reviews)
Application
Western Blot(WB) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 12-Jun-2015
Score :
Application Tested : WB
Sample Species : Hu
Sample : 3A6 cells
Amount used : 30μg
Blocking : 3% BSA in TBST, 25°C, 1Hr
Primary Antibody : 1:2000, 4°C, 16Hr
SDS
PBS.pdf
Package List Price ($)
$ 329
$ 169