Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GEF-H1 antibody [GT336] (GTX629398) diluted at 1:500. The HRP-conjugated anti-mouset IgG antibody (GTX213111-01) was used to detect the primary antibody.

GEF-H1 antibody [GT336] detects GEF-H1 protein at cytoskeleton by immunofluorescent analysis.
Sample: MDA-MB-231 cells were fixed in methanol for 5 min.
Green: GEF-H1 protein stained by GEF-H1 antibody [GT336] (GTX629398) diluted at 1:50.

GEF-H1 antibody [GT336] detects GEF-H1 protein by Western blot analysis.
A. 30 μg C8D30 whole cell lysate/extract
B. 30 μg BCL-1 whole cell lysate/extract
C. 30 μg Raw264.7 whole cell lysate/extract
D. 30 μg C2C12 whole cell lysate/extract
5 % SDS-PAGE
GEF-H1 antibody [GT336] (GTX629398) dilution: 1:500

GEF-H1 antibody [GT336] detects GEF-H1 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at room temperature.
Green: GEF-H1 protein stained by GEF-H1 antibody [GT336] (GTX629398) diluted at 1:50.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

GEF-H1 antibody [GT336] detects GEF-H1 protein by Western blot analysis.
A. 30 μg Rat-2 whole cell lysate/extract
5 % SDS-PAGE
GEF-H1 antibody [GT336] (GTX629398) dilution: 1:500

GEF-H1 antibody [GT336] detects GEF-H1 protein by Western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg A431 whole cell lysate/extract
C. 30 μg HeLa whole cell lysate/extract
7.5 % SDS-PAGE
GEF-H1 antibody [GT336] (GTX629398) dilution: 1:500