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GEF-H1 antibody

Anti-GEF-H1 antibody used in Western Blot (WB). GTX125893
Anti-GEF-H1 antibody used in Western Blot (WB). GTX125893
Anti-GEF-H1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX125893
Anti-GEF-H1 antibody used in IHC (Paraffin sections) (IHC-P). GTX125893
Anti-GEF-H1 antibody used in IHC (Frozen sections) (IHC-Fr). GTX125893
Anti-GEF-H1 antibody used in Immunoprecipitation (IP). GTX125893
Anti-GEF-H1 antibody used in Western Blot (WB). GTX125893
Anti-GEF-H1 antibody used in IHC (Paraffin sections) (IHC-P). GTX125893
Anti-GEF-H1 antibody used in IHC (Paraffin sections) (IHC-P). GTX125893
Anti-GEF-H1 antibody used in IHC (Paraffin sections) (IHC-P). GTX125893
Anti-GEF-H1 antibody used in Western Blot (WB). GTX125893
Anti-GEF-H1 antibody used in Western Blot (WB). GTX125893
Anti-GEF-H1 antibody used in Western Blot (WB). GTX125893
Anti-GEF-H1 antibody used in Western Blot (WB). GTX125893

Cat No. GTX125893

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IHC-Fr, IP
Reactivity Human, Mouse, Rat
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

112 kDa. ( Note )

Positive Control

293T , A431 , BCL-1 , C2C12 , C8D30 , GL261 , HeLa , HepG2 , NIH-3T3 , Neuro 2A , Raw264.7 , hMSC-3A6 , PC-12 , Rat2 , *mouse fibroblast

Predict Reactivity

Bovine, Dog, Pig(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.51 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human GEF-H1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Rho/Rac guanine nucleotide exchange factor 2,GEF,GEF-H1,GEFH1,LFP40,NEDMHM,P40

Cellular Localization

Cytoplasm , Cell junction , tight junction , Golgi apparatus , Spindle

Background

Rho GTPases play a fundamental role in numerous cellular processes that are initiated by extracellular stimuli that work through G protein coupled receptors. The encoded protein may form complex with G proteins and stimulate rho-dependent signals. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-GEF-H1 antibody used in Western Blot (WB). GTX125893

GTX125893 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GEF-H1 antibody (GTX125893) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-GEF-H1 antibody used in Western Blot (WB). GTX125893

GTX125893 WB Image

Sample (10 µg of whole cell lysate)
A: hMSC-3A6
B: siRNA of ARHGEF2/GEF-H1
8% SDS PAGE
GTX125893 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-GEF-H1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX125893

GTX125893 ICC/IF Image

GEF-H1 antibody detects ARHGEF2 protein at Cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in -20℃ 100% MeOH for 5 min.
Green: ARHGEF2 protein stained by GEF-H1 antibody (GTX125893) diluted at 1:500.
Blue: Hoechst 33343 staining.

Anti-GEF-H1 antibody used in IHC (Paraffin sections) (IHC-P). GTX125893

GTX125893 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human gastric cancer, using GEF-H1(GTX125893) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-GEF-H1 antibody used in IHC (Frozen sections) (IHC-Fr). GTX125893

GTX125893 IHC-Fr Image

GEF-H1 antibody detects GEF-H1 protein by immunohistochemical analysis.
Sample: Frozen-sectioned mouse mouse cerebellum.
Green: GEF-H1 stained by GEF-H1 antibody (GTX125893) diluted at 1:250.
Red: NF-H, stained by NF-H antibody [GT114] (GTX634289) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 10 min

Anti-GEF-H1 antibody used in Immunoprecipitation (IP). GTX125893

GTX125893 IP Image

Immunoprecipitation of GEF-H1 protein from 293T whole cell extracts using 5 μg of GEF-H1 antibody (GTX125893).
Western blot analysis was performed using GEF-H1 antibody (GTX125893).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-GEF-H1 antibody used in Western Blot (WB). GTX125893

GTX125893 WB Image

GEF-H1 antibody detects GEF-H1 protein by western blot analysis.
A. 30 µg Raw264.7 whole cell lysate/extract
B. 30 µg C2C12 whole cell lysate/extract
5% SDS-PAGE
GEF-H1 antibody (GTX125893) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-GEF-H1 antibody used in IHC (Paraffin sections) (IHC-P). GTX125893

GTX125893 IHC-P Image

GEF-H1 antibody detects GEF-H1 protein at cytosol on mouse testis by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
GEF-H1 antibody (GTX125893) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-GEF-H1 antibody used in IHC (Paraffin sections) (IHC-P). GTX125893

GTX125893 IHC-P Image

GEF-H1 antibody detects GEF-H1 protein at cytosol on rat hind brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat hind brain.
GEF-H1 antibody (GTX125893) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-GEF-H1 antibody used in IHC (Paraffin sections) (IHC-P). GTX125893

GTX125893 IHC-P Image

GEF-H1 antibody detects GEF-H1 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
GEF-H1 stained by GEF-H1 antibody (GTX125893) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-GEF-H1 antibody used in Western Blot (WB). GTX125893

GTX125893 WB Image

GEF-H1 antibody detects GEF-H1 protein by western blot analysis.
A. 30 µg 293T whole cell lysate/extract
B. 30 µg A431 whole cell lysate/extract
C. 30 µg HeLa whole cell lysate/extract
D. 30 µg HepG2 whole cell lysate/extract
5% SDS-PAGE
GEF-H1 antibody (GTX125893) dilution: 1:2000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-GEF-H1 antibody used in Western Blot (WB). GTX125893

GTX125893 WB Image

GEF-H1 antibody detects GEF-H1 protein by western blot analysis.
A. 30 µg Neuro2A whole cell lysate/extract
B. 30 µg GL261 whole cell lysate/extract
C. 30 µg C8D30 whole cell lysate/extract
D. 30 µg NIH-3T3 whole cell lysate/extract
E. 30 µg BCL-1 whole cell lysate/extract
F. 30 µg Raw 264.7 whole cell lysate/extract
G. 30 µg C2Cl2 whole cell lysate/extract
5% SDS-PAGE
GEF-H1 antibody (GTX125893) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-GEF-H1 antibody used in Western Blot (WB). GTX125893

GTX125893 WB Image

Sample (30 µg of whole cell lysate)
A: 293T
B: A431
C: HeLa
5% SDS PAGE
GTX125893 diluted at 1:2000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-GEF-H1 antibody used in Western Blot (WB). GTX125893

GTX125893 WB Image

Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GEF-H1 antibody (GTX125893) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE
REVIEW

GEF-H1 antibody Cat No. GTX125893

Rating ( Average 3.5 based on 2 users reviews)
Application
Western Blot(WB) ( Average 3 based on 1 users reviews)
Immunoprecipitation(IP) ( Average 4 based on 1 users reviews)
Date : Anonymous submitted on 13-Mar-2014
Score :
Application Tested : WB
Sample Species : Ms
Sample : mouse fibroblast cells
Amount used : 30μg
Blocking : 3%BSA-TBS, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 16Hr
Date : Anonymous submitted on 17-Oct-2012
Score :
Application Tested : IP
Sample Species : Hu
Sample : 3A6 cells
Amount used : 700μg for IP
Primary Antibody : 1:1000, 4°C, 16Hr
Figure Note : 3ug for IP
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169