GeneTex
United States (US)

GEM antibody [N1], N-term

GTX100287_WB_19011506_702.jpg
GTX100287_39428_IHC_19011506_715.jpg

Cat No. GTX100287

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

34 kDa. ( Note )

Positive Control

293T

Predict Reactivity

Mouse, Rat(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human GEM. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

KIR antibody, MGC26294 antibody, GEM antibody, RAS-like protein KIR antibody, GTP-binding protein overexpressed in skeletal muscle antibody, GTP-binding protein GEM antibody, kinase-inducible Ras-like protein antibody, GTP-binding mitogen-induced T-cell protein antibody, GTP binding protein overexpressed in skeletal muscle antibody

Cellular Localization

Cell membrane; Peripheral membrane protein; Cytoplasmic side

Background

The protein encoded by this gene belongs to the RAD/GEM family of GTP-binding proteins. It is associated with the inner face of the plasma membrane and could play a role as a regulatory protein in receptor-mediated signal transduction. Alternative splicing occurs at this locus and two transcript variants encoding the same protein have been identified. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX100287_WB_19011506_702.jpg

GTX100287 WB Image

Sample (30 ug of whole cell lysate)
A: 293T
12% SDS PAGE
GTX100287 diluted at 1:500

GTX100287_39428_IHC_19011506_715.jpg

GTX100287 IHC-P Image

GEM antibody [N1], N-term detects GEM protein at membrane on human lung by immunohistochemical analysis.
Sample: Paraffin-embedded lung.
GEM antibody [N1], N-term (GTX100287) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169