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GFAP antibody [ASTRO/1974R]

Anti-GFAP antibody [ASTRO/1974R] used in Flow cytometry (FACS). GTX02644
Anti-GFAP antibody [ASTRO/1974R] used in IHC (Paraffin sections) (IHC-P). GTX02644

Cat. No. GTX02644

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 ASTRO/1974R

Isotype

 IgG

Application

 IHC-P, FACS

Reactivity

 Human, Mouse, Rat, Rabbit, Bovine, Chicken, Pig
Package
100 μg ($529)
See all GFAP products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
IHC-P 1-2 μg/ml
FACS 1-2 μg/million cells

Note :

IHC-P
Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris buffer with 1mM EDTA (pH 9.0) for 45 min at 95ºC followed by cooling at RT for 20 minutes.
Not tested in other applications.

Calculated MW

50 kDa. ( Note )

Product Note

It shows no cross-reaction with other intermediate filament proteins.

PROPERTIES

Form

Liquid

Buffer

10mM PBS, 0.05% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.

Concentration

200 μg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant human full-length GFAP protein

Purification

Protein A/G purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

glial fibrillary acidic protein , ALXDRD

Cellular Localization

Cytoplasm

Background

This gene encodes one of the major intermediate filament proteins of mature astrocytes. It is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Oct 2008]

Database

Research Area

DATA IMAGES

Anti-GFAP antibody [ASTRO/1974R] used in Flow cytometry (FACS). GTX02644

GTX02644 FACS Image

FACS analysis of T98G cells using GTX02644 GFAP antibody [ASTRO/1974R].
Blue : Primary antibody
Red : Isotype control

Anti-GFAP antibody [ASTRO/1974R] used in IHC (Paraffin sections) (IHC-P). GTX02644

GTX02644 IHC-P Image

IHC-P analysis of human cerebellum tissue section using GTX02644 GFAP antibody [ASTRO/1974R].

REFERENCE

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REVIEW

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Package List Price ($)
$ 529