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GFAP antibody, C-term

Anti-GFAP antibody, C-term used in IHC (Paraffin sections) (IHC-P). GTX89226
Anti-GFAP antibody, C-term used in Western Blot (WB). GTX89226
Anti-GFAP antibody, C-term used in IHC (Paraffin sections) (IHC-P). GTX89226
Anti-GFAP antibody, C-term used in IHC (Frozen sections) (IHC-Fr). GTX89226
Anti-GFAP antibody, C-term used in Western Blot (WB). GTX89226
Anti-GFAP antibody, C-term used in ELISA (ELISA). GTX89226

Cat. No. GTX89226

Reference 1

Host

 Goat

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, IHC-Fr, ELISA, Sandwich ELISA

Reactivity

 Human, Mouse, Rat
Package
100 μg ($399)
See all GFAP products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.003-0.03μg/ml
IHC-P Assay dependent
IHC-Fr Assay dependent
ELISA Assay dependent
Sandwich ELISA Assay dependent
Not tested in other applications.

Calculated MW

50 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX89226-PEP).

Predict Reactivity

Dog(>80% identity)

PROPERTIES

Form

Liquid

Buffer

TBS, 0.5% BSA

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence C-DGEVIKESKQEHKD, from the C Terminus of the protein sequence according to NP_002046.1.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

glial fibrillary acidic protein , ALXDRD

Cellular Localization

Cytoplasm

Background

This gene encodes one of the major intermediate filament proteins of mature astrocytes. It is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Oct 2008]

Database

Research Area

DATA IMAGES

Anti-GFAP antibody, C-term used in IHC (Paraffin sections) (IHC-P). GTX89226

GTX89226 IHC-P Image

IHC-P analysis of human cerebellum using GTX89226 GFAP antibody, C-term.
Antigen retrieval : citrate buffer pH 6
Dilution : 2μg/ml

Anti-GFAP antibody, C-term used in Western Blot (WB). GTX89226

GTX89226 WB Image

WB analysis of mouse (Lane A) and rat (Lane B) brain lysate (35μg protein in RIPA buffer) using GTX89226 GFAP antibody, C-term.
Dilution : 0.003μg/ml

Dilution : 0.003μg/ml

Anti-GFAP antibody, C-term used in IHC (Paraffin sections) (IHC-P). GTX89226

GTX89226 IHC-P Image

IHC-P analysis of human cortex using GTX89226 GFAP antibody, C-term.
Antigen retrieval : citrate buffer pH 6
Dilution : 5μg/ml

Anti-GFAP antibody, C-term used in IHC (Frozen sections) (IHC-Fr). GTX89226

GTX89226 IHC-Fr Image

IHC-Fr analysis of PFA-perfused human hypothalamus using GTX89226 GFAP antibody, C-term.
Antigen retrieval : citrate buffer pH 6 at 80C for 30min
Dilution : 0.01μg/ml

Anti-GFAP antibody, C-term used in Western Blot (WB). GTX89226

GTX89226 WB Image

WB analysis of human cerebellum (Lane A) and cerebral cortex (Lane B) lysate (35μg protein in RIPA buffer) using GTX89226 GFAP antibody, C-term.
Dilution : 0.001μg/ml

Dilution : 0.001μg/ml

Anti-GFAP antibody, C-term used in ELISA (ELISA). GTX89226

GTX89226 ELISA Image

ELISA analysis of GFAP protein using GTX89226 GFAP antibody, C-term.
Capture : GTX108711 (5μg/ml)
Detection : GTX89226 (5μg/ml)

REFERENCE

Application Reference

Fuyuki A et al. J Vet Med Sci 2023; 85 (3) : 266-270 Expression of prosaposin and its G protein-coupled receptor (GPR) 37 in mouse cochlear and vestibular nuclei.
Application : IHC-Fr
Reactivity : Mouse
Submit a Reference

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399