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GFAP antibody [GA-5]

Anti-GFAP antibody [GA-5] used in Flow cytometry (FACS). GTX34759
Anti-GFAP antibody [GA-5] used in IHC (Paraffin sections) (IHC-P). GTX34759
Anti-GFAP antibody [GA-5] used in Western Blot (WB). GTX34759
Anti-GFAP antibody [GA-5] used in Western Blot (WB). GTX34759

Cat. No. GTX34759

Reference 1

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 GA-5

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P, FACS

Reactivity

 Human, Mouse, Rat, Rabbit, Bovine, Chicken, Pig
Package
100 μg ($529)
See all GFAP products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1-2μg/ml
ICC/IF 1-2μg/ml
IHC-P 1-2μg/ml for 30 minutes at RT
FACS 1-2μg/10⁶ cells

Note :

IHC-P
Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95ºC followed by cooling at RT for 20 minutes.
Not tested in other applications.

Product Note

This MAb recognizes a protein of ~50kDa which is identified as Glial Fibrillary Acidic Protein (GFAP). It shows no cross-reaction with other intermediate filament proteins.

PROPERTIES

Form

Liquid

Buffer

10mM PBS, 0.05% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Pig

Immunogen

GFAP isolated from pig spinal cord

Purification

Protein A/G purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Database

Research Area

DATA IMAGES

Anti-GFAP antibody [GA-5] used in Flow cytometry (FACS). GTX34759

GTX34759 FACS Image

FACS analysis of T98G cells using GTX34759 GFAP antibody [GA-5].
Blue : Primary antibody
Red : Isotype control

Anti-GFAP antibody [GA-5] used in IHC (Paraffin sections) (IHC-P). GTX34759

GTX34759 IHC-P Image

IHC-P analysis of human cerebellum tissue using GTX34759 GFAP antibody [GA-5].

Anti-GFAP antibody [GA-5] used in Western Blot (WB). GTX34759

GTX34759 WB Image

WB analysis of human brain tissue lysate using GTX34759 GFAP antibody [GA-5].

Anti-GFAP antibody [GA-5] used in Western Blot (WB). GTX34759

GTX34759 WB Image

WB analysis of human brain tissue lysate using GTX34759 GFAP antibody [GA-5].

REFERENCE

Application Reference

XX Jia et al. Research Square 2024; Aberrance of GAP43/p-GAP43 closely associates with the pathology of neuron loss in prion-infected rodent models
Application : IHC-P
Submit a Reference

REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 529