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GFAP antibody [GA-5]

Anti-GFAP antibody [GA-5] used in IHC (Paraffin sections) (IHC-P). GTX34759
Anti-GFAP antibody [GA-5] used in Western Blot (WB). GTX34759

Cat No. GTX34759

Host

Mouse

Clonality

Monoclonal

Clone Name

GA-5

Isotype

IgG1

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Mouse, Rat, Rabbit, Bovine, Chicken, Pig
Package
100 μg ($429)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.5-1ug/ml
ICC/IF 1-2ug/ml
IHC-P 0.25-0.5ug/ml for 30 minutes at RT

Note :

IHC-P
Boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes

Not tested in other applications.

Calculated MW

50 kDa. ( Note )

Specificity/Sensitivity

This MAb recognizes a protein of ~50kDa which is identified as Glial Fibrillary Acidic Protein (GFAP). It shows no cross-reaction with other intermediate filament proteins. GFAP is specifically found in astroglia. GFAP is a very popular marker for localizing benign astrocyte and neoplastic cells of glial origin in the central nervous system. Antibody to GFAP is useful in differentiating primary gliomas from metastatic lesions in the brain and for documenting astrocytic differentiation in tumors outside the CNS.

PROPERTIES

Form

Liquid

Buffer

10mM PBS with 0.05% BSA, 0.05% azide (Please contact us for PBS only format)

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml

Antigen Species

Pig

Immunogen

GFAP isolated from pig spinal cord

Purification

Ab purified from Bioreactor Concentrate by Protein A/G

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Glial Fibrillary Acidic Protein , Alxdrd , Gfap

Background

This gene encodes one of the major intermediate filament proteins of mature astrocytes. It is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Oct 2008]

Database

Research Area

DATA IMAGES

Anti-GFAP antibody [GA-5] used in IHC (Paraffin sections) (IHC-P). GTX34759

GTX34759 IHC-P Image

Formalin-fixed, paraffin-embedded human Cerebellum stained with GFAP Monoclonal Antibody (GA-5).

Anti-GFAP antibody [GA-5] used in Western Blot (WB). GTX34759

GTX34759 WB Image

Western Blot of human Cerebellum Lysate using GFAP Monoclonal Antibody (GA-5).

REFERENCE

There are currently no references for GFAP antibody [GA-5] (GTX34759). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for GFAP antibody [GA-5] (GTX34759). Be the first to share your experience with this product.
Package List Price ($)
$ 429