GeneTex
United States (US)

GFAP antibody [GF-01]

GTX27806_IHC-P_18121410_582.jpg
GFAP-antibody-GF-01__GTX27806-1_18121410_251.jpg

Cat No. GTX27806

Host Mouse
Clonality Monoclonal
Clone Name GF-01
Isotype IgG1
Application WB, ICC/IF, IHC-P, IHC-Fr, IP
Reactivity Human, Cat, Pig
APPLICATION

Application Note

ICC: Use at an assay dependant dilution. IHC-P: Use at an assay dependant concentration. The antibody strongly stains astrocytes in human brain tissue sections but it is essentially negative on mouse and rat tissues. IHC-Fr: Use at an assay dependant concentration. IP: Use at an assay dependant dilution. WB: Use at an assay dependant concentration. Positive in dibutyryl cAMP stimulated human glioma cell line U333 CG/343 MG, but negative in rat glioma cell line C6. Positive in pig brain lysate, but negative in mouse brain lysate. Optimal dilutions/concentrations should be determined by the end user.
PROPERTIES

Form

Liquid

Buffer

Phosphate buffered saline pH 7.4 containing 15mM sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Pig

Immunogen

A pellet of pig brain cold-stable proteins after depolymerization of microtubules.

Purification

Protein A purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

,

Background

Glial fibrillary acidic protein (GFAP) is a member of the class III intermediate filament protein family. It is heavily and specifically expressed in astrocytes and certain other astroglia in the central nervous system, in satellite cells in peripheral ganglia, and in non-myelinating Schwann cells in peripheral nerves. In addition neural stem cells frequently strongly express GFAP. Antibodies to GFAP are therefore very useful as markers of astrocytic cells. In addition many types of brain tumor, presumably derived from astrocytic cells, heavily express GFAP. GFAP is also found in the lens epithelium, Kupffer cells of the liver, in some cells in salivary tumors and has been reported in erythrocytes.

Database

Research Area

DATA IMAGES
GTX27806_IHC-P_18121410_582.jpg

GTX27806 IHC-P Image

IHC-P analysis of human brain cortex using GFAP antibody [GF-01].

GFAP-antibody-GF-01__GTX27806-1_18121410_251.jpg

GTX27806 IHC-P Image

Immunohistochemistry staining of human cerebellum (paraffin-embedded sections) with anti-GFAP (clone GF-01) (GTX27806).

Package List Price ($)
$ 319