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GFAP antibody [HL1307]

Anti-GFAP antibody [HL1307] used in Western Blot (WB). GTX636725
Anti-GFAP antibody [HL1307] used in IHC (Paraffin sections) (IHC-P). GTX636725
Anti-GFAP antibody [HL1307] used in IHC (Paraffin sections) (IHC-P). GTX636725
Anti-GFAP antibody [HL1307] used in IHC (Frozen sections) (IHC-Fr). GTX636725
Anti-GFAP antibody [HL1307] used in Western Blot (WB). GTX636725
Anti-GFAP antibody [HL1307] used in IHC (Paraffin sections) (IHC-P). GTX636725
Anti-GFAP antibody [HL1307] used in Western Blot (WB). GTX636725
Anti-GFAP antibody [HL1307] used in Western Blot (WB). GTX636725
Anti-GFAP antibody [HL1307] used in IHC (Paraffin sections) (IHC-P). GTX636725
Anti-GFAP antibody [HL1307] used in IHC (Paraffin sections) (IHC-P). GTX636725
Anti-GFAP antibody [HL1307] used in IHC (Frozen sections) (IHC-Fr). GTX636725

Cat. No. GTX636725

Host

Rabbit

Clonality

Monoclonal

Clone Name

HL1307

Isotype

IgG

Application

WB, IHC-P, IHC-Fr

Reactivity

Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
IHC-P 1:100-1:1000
IHC-Fr Assay dependent
Not tested in other applications.

Calculated MW

50 kDa. ( Note )

Predict Reactivity

Bovine, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human GFAP. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_2910057

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

glial fibrillary acidic protein , ALXDRD

Cellular Localization

Cytoplasm

Background

This gene encodes one of the major intermediate filament proteins of mature astrocytes. It is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Oct 2008]

Database

Research Area

DATA IMAGES

Anti-GFAP antibody [HL1307] used in Western Blot (WB). GTX636725

GTX636725 WB Image

Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membranes were blotted with GFAP antibody [HL1307] (GTX636725) diluted at 1:3000 and competitor's antibody (CST 34001) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-GFAP antibody [HL1307] used in IHC (Paraffin sections) (IHC-P). GTX636725

GTX636725 IHC-P Image

GFAP antibody [HL1307] detects GFAP protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
GFAP stained by GFAP antibody [HL1307] (GTX636725) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-GFAP antibody [HL1307] used in IHC (Paraffin sections) (IHC-P). GTX636725

GTX636725 IHC-P Image

GFAP antibody [HL1307] detects GFAP protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
GFAP stained by GFAP antibody [HL1307] (GTX636725) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-GFAP antibody [HL1307] used in IHC (Frozen sections) (IHC-Fr). GTX636725

GTX636725 IHC-Fr Image

GFAP antibody [HL1307] detects GFAP protein at cytoplasm by immunohistochemical analysis.
Sample: Frozen-sectioned mouse spinal cord.
Green: GFAP stained by GFAP antibody [HL1307] (GTX636725) diluted at 1:100.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-GFAP antibody [HL1307] used in Western Blot (WB). GTX636725

GTX636725 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GFAP antibody [HL1307] (GTX636725) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-GFAP antibody [HL1307] used in IHC (Paraffin sections) (IHC-P). GTX636725

GTX636725 IHC-P Image

GFAP antibody [HL1307] detects GFAP protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
GFAP stained by GFAP antibody [HL1307] (GTX636725) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-GFAP antibody [HL1307] used in Western Blot (WB). GTX636725

GTX636725 WB Image

Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GFAP antibody [HL1307] (GTX636725) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-GFAP antibody [HL1307] used in Western Blot (WB). GTX636725

GTX636725 WB Image

Human tissue extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with GFAP antibody [HL1307] (GTX636725) diluted at 1:100000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-GFAP antibody [HL1307] used in IHC (Paraffin sections) (IHC-P). GTX636725

GTX636725 IHC-P Image

GFAP antibody [HL1307] detects GFAP protein by immunohistochemical analysis.
Sample: Paraffin-embedded rat tissue.
GFAP stained by GFAP antibody [HL1307] (GTX636725) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-GFAP antibody [HL1307] used in IHC (Paraffin sections) (IHC-P). GTX636725

GTX636725 IHC-P Image

GFAP antibody [HL1307] detects GFAP protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
GFAP stained by GFAP antibody [HL1307] (GTX636725) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-GFAP antibody [HL1307] used in IHC (Frozen sections) (IHC-Fr). GTX636725

GTX636725 IHC-Fr Image

GFAP antibody [HL1307] detects GFAP protein at glia cells by immunohistochemical analysis.
Sample: Frozen-sectioned mouse cerebellum.
Green: GFAP stained by GFAP antibody [HL1307] (GTX636725) diluted at 1:200.
Blue: Fluoroshield with DAPI (GTX30920).

REFERENCE

There are currently no references for GFAP antibody [HL1307] (GTX636725). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for GFAP antibody [HL1307] (GTX636725). Be the first to share your experience with this product.
Package List Price ($)
$ 399
$ 169
Custom Conjugations