GFAP antibody [HL1307]

Name: GFAP antibody [HL1307]
Brand: GeneTex
SKU: GTX636725
Price: 399.00 USD

Anti-GFAP antibody [HL1307] used in IHC (Paraffin sections) (IHC-P). GTX636725

Anti-GFAP antibody [HL1307] used in IHC (Frozen sections) (IHC-Fr). GTX636725

Anti-GFAP antibody [HL1307] used in Western Blot (WB). GTX636725

Cat. No. GTX636725

Host	Rabbit
Clonality	Monoclonal
Clone Name	HL1307
Isotype	IgG
Application	WB, IHC-P, IHC-Fr
Reactivity	Human, Mouse, Rat
Package	100 μl ($399), 25 μl ($169)

See all GFAP products

Datasheet

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:1000-1:10000
IHC-P	1:100-1:1000
IHC-Fr	Assay dependent

Not tested in other applications.

Calculated MW

50 kDa. ( Note )

Predict Reactivity

Bovine, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human GFAP. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_2910057

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

glial fibrillary acidic protein , ALXDRD

Cellular Localization

Cytoplasm

Background

This gene encodes one of the major intermediate filament proteins of mature astrocytes. It is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Oct 2008]

Database

Gene ID: 2670 GFAP
UniProt: P14136 GFAP

Research Area

DATA IMAGES

GTX636725 IHC-P Image

GFAP antibody [HL1307] detects GFAP protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
GFAP stained by GFAP antibody [HL1307] (GTX636725) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX636725 IHC-Fr Image

GFAP antibody [HL1307] detects GFAP protein at glia cells by immunohistochemical analysis.
Sample: Frozen-sectioned mouse cerebellum.
Green: GFAP stained by GFAP antibody [HL1307] (GTX636725) diluted at 1:200.
Blue: Fluoroshield with DAPI (GTX30920).

GTX636725 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GFAP antibody [HL1307] (GTX636725) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX636725 IHC-P Image

GFAP antibody [HL1307] detects GFAP protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
GFAP stained by GFAP antibody [HL1307] (GTX636725) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX636725 WB Image

Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GFAP antibody [HL1307] (GTX636725) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX636725 WB Image

Human tissue extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with GFAP antibody [HL1307] (GTX636725) diluted at 1:100000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX636725 IHC-Fr Image

GFAP antibody [HL1307] detects GFAP protein at cytoplasm by immunohistochemical analysis.
Sample: Frozen-sectioned mouse spinal cord.
Green: GFAP stained by GFAP antibody [HL1307] (GTX636725) diluted at 1:100.
Blue: Fluoroshield with DAPI (GTX30920).

GTX636725 IHC-P Image

GFAP antibody [HL1307] detects GFAP protein by immunohistochemical analysis.
Sample: Paraffin-embedded rat tissue.
GFAP stained by GFAP antibody [HL1307] (GTX636725) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

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REVIEW

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Package

List Price ($)

100 μl

$ 399

25 μl

$ 169

Qty Custom Conjugations

GFAP antibody [HL1307]

Cat. No. GTX636725

Host

Clonality

Clone Name

Isotype

Application

Reactivity

APPLICATION

Application Note

Calculated MW

Predict Reactivity

PROPERTIES

Form

Buffer

Preservative

Storage

Concentration

Antigen Species

Immunogen

Purification

Conjugation

RRID

Note

TARGET

Synonyms

Cellular Localization

Background

Database

Research Area

DATA IMAGES

GTX636725 IHC-P Image

GTX636725 IHC-Fr Image

GTX636725 WB Image

GTX636725 IHC-P Image

GTX636725 WB Image

GTX636725 WB Image

GTX636725 IHC-Fr Image

GTX636725 IHC-P Image

GTX636725 IHC-P Image

GTX636725 IHC-P Image

REFERENCE

REVIEW

Product

Research

Support

Company

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All products are for research use only—Not for use in diagnostic or therapeutic applications.