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GFAP antibody [HL1307]

Anti-GFAP antibody [HL1307] used in IHC (Paraffin sections) (IHC-P). GTX636725
Anti-GFAP antibody [HL1307] used in IHC (Paraffin sections) (IHC-P). GTX636725
Anti-GFAP antibody [HL1307] used in Western Blot (WB). GTX636725
Anti-GFAP antibody [HL1307] used in Western Blot (WB). GTX636725
Anti-GFAP antibody [HL1307] used in IHC (Frozen sections) (IHC-Fr). GTX636725
Anti-GFAP antibody [HL1307] used in Western Blot (WB). GTX636725
Anti-GFAP antibody [HL1307] used in IHC (Paraffin sections) (IHC-P). GTX636725
Anti-GFAP antibody [HL1307] used in IHC (Paraffin sections) (IHC-P). GTX636725
Anti-GFAP antibody [HL1307] used in IHC (Paraffin sections) (IHC-P). GTX636725

Cat. No. GTX636725

Host

Rabbit

Clonality

Monoclonal

Clone Name

HL1307

Isotype

IgG

Application

WB, IHC-P, IHC-Fr

Reactivity

Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
IHC-P 1:100-1:1000
IHC-Fr Assay dependent
Not tested in other applications.

Calculated MW

50 kDa. ( Note )

Predict Reactivity

Bovine, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human GFAP. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_2910057

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

glial fibrillary acidic protein , ALXDRD

Cellular Localization

Cytoplasm

Background

This gene encodes one of the major intermediate filament proteins of mature astrocytes. It is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Oct 2008]

Database

Research Area

DATA IMAGES

Anti-GFAP antibody [HL1307] used in IHC (Paraffin sections) (IHC-P). GTX636725

GTX636725 IHC-P Image

GFAP antibody [HL1307] detects GFAP protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
GFAP stained by GFAP antibody [HL1307] (GTX636725) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-GFAP antibody [HL1307] used in IHC (Paraffin sections) (IHC-P). GTX636725

GTX636725 IHC-P Image

GFAP antibody [HL1307] detects GFAP protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
GFAP stained by GFAP antibody [HL1307] (GTX636725) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-GFAP antibody [HL1307] used in Western Blot (WB). GTX636725

GTX636725 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GFAP antibody [HL1307] (GTX636725) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-GFAP antibody [HL1307] used in Western Blot (WB). GTX636725

GTX636725 WB Image

Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GFAP antibody [HL1307] (GTX636725) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-GFAP antibody [HL1307] used in IHC (Frozen sections) (IHC-Fr). GTX636725

GTX636725 IHC-Fr Image

GFAP antibody [HL1307] detects GFAP protein at glia cells by immunohistochemical analysis.
Sample: Frozen-sectioned mouse cerebellum.
Green: GFAP stained by GFAP antibody [HL1307] (GTX636725) diluted at 1:200.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-GFAP antibody [HL1307] used in Western Blot (WB). GTX636725

GTX636725 WB Image

Human tissue extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with GFAP antibody [HL1307] (GTX636725) diluted at 1:100000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-GFAP antibody [HL1307] used in IHC (Paraffin sections) (IHC-P). GTX636725

GTX636725 IHC-P Image

GFAP antibody [HL1307] detects GFAP protein by immunohistochemical analysis.
Sample: Paraffin-embedded rat tissue.
GFAP stained by GFAP antibody [HL1307] (GTX636725) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-GFAP antibody [HL1307] used in IHC (Paraffin sections) (IHC-P). GTX636725

GTX636725 IHC-P Image

GFAP antibody [HL1307] detects GFAP protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
GFAP stained by GFAP antibody [HL1307] (GTX636725) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-GFAP antibody [HL1307] used in IHC (Paraffin sections) (IHC-P). GTX636725

GTX636725 IHC-P Image

GFAP antibody [HL1307] detects GFAP protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
GFAP stained by GFAP antibody [HL1307] (GTX636725) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

There are currently no references for GFAP antibody [HL1307] (GTX636725). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for GFAP antibody [HL1307] (GTX636725). Be the first to share your experience with this product.
Package List Price ($)
$ 399
$ 169
Custom Conjugations