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GFAP antibody [SPM248]

Anti-GFAP antibody [SPM248] used in Flow cytometry (FACS). GTX34756
Anti-GFAP antibody [SPM248] used in Western Blot (WB). GTX34756
Anti-GFAP antibody [SPM248] used in IHC (Paraffin sections) (IHC-P). GTX34756

Cat No. GTX34756

Host

Mouse

Clonality

Monoclonal

Clone Name

SPM248

Isotype

IgG1

Application

WB, ICC/IF, IHC-P, FACS

Reactivity

Human, Mouse, Rat, Rabbit, Bovine, Chicken, Pig
Package
100 μg ($339)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1-2μg/ml
ICC/IF 1-2μg/ml
IHC-P 1-2μg/ml for 30 minutes at RT
FACS 1-2μg/10⁶ cells

Note :

IHC-P
Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95ºC followed by cooling at RT for 20 minutes.

Not tested in other applications.

Specificity/Sensitivity

This MAb recognizes a protein of ~50kDa which is identified as Glial Fibrillary Acidic Protein (GFAP). It shows no cross-reaction with other intermediate filament proteins.

PROPERTIES

Form

Liquid

Buffer

10mM PBS, 0.05% BSA, 0.05% sodium azide (Please contact us for PBS only format)

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Pig

Immunogen

GFAP isolated from pig spinal cord

Purification

Protein A/G purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

DATA IMAGES

Anti-GFAP antibody [SPM248] used in Flow cytometry (FACS). GTX34756

GTX34756 FACS Image

FACS analysis of T98G cells using GTX34756 GFAP antibody [SPM248].
Blue : Primary antibody
Red : Isotype control

Anti-GFAP antibody [SPM248] used in Western Blot (WB). GTX34756

GTX34756 WB Image

WB analysis of human brain tissue lysate using GTX34756 GFAP antibody [SPM248].

Anti-GFAP antibody [SPM248] used in IHC (Paraffin sections) (IHC-P). GTX34756

GTX34756 IHC-P Image

IHC-P analysis of human schwanoma tissue using GTX34756 GFAP antibody [SPM248].

REFERENCE

There are currently no references for GFAP antibody [SPM248] (GTX34756). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for GFAP antibody [SPM248] (GTX34756). Be the first to share your experience with this product.
SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 339