GeneTex
United States (US)

GFAP antibody [SPM507]

GTX34757_20170712_IHC-P_18121410_475.jpg

Cat No. GTX34757

Host Mouse
Clonality Monoclonal
Clone Name SPM507
Isotype IgG1
Application IHC-P
Reactivity Human, Mouse, Rat, Rabbit, Bovine, Chicken, Pig
APPLICATION

Application Note

IHC-P:0.25-0.5ug/ml for 30 minutes at RT.

Calculated MW

50 kDa. ( Note )

Specificity/Sensitivity

This MAb recognizes a protein of ~50kDa which is identified as Glial Fibrillary Acidic Protein (GFAP). It shows no cross-reaction with other intermediate filament proteins. GFAP is specifically found in astroglia. GFAP is a very popular marker for localizing benign astrocyte and neoplastic cells of glial origin in the central nervous system. Antibody to GFAP is useful in differentiating primary gliomas from metastatic lesions in the brain and for documenting astrocytic differentiation in tumors outside the CNS.
PROPERTIES

Form

Liquid

Buffer

Prepared in 10mM PBS with 0.05% BSA and 0.05% azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Pig

Immunogen

GFAP isolated from pig spinal cord

Purification

Ab purified from Bioreactor Concentrate by Protein A/G

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Glial Fibrillary Acidic Protein,Alxdrd,Gfap

Background

This gene encodes one of the major intermediate filament proteins of mature astrocytes. It is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Oct 2008]

Database

Research Area

DATA IMAGES
GTX34757_20170712_IHC-P_18121410_475.jpg

GTX34757 IHC-P Image

Formalin-fixed, paraffin-embedded human Cerebellum stained with GFAP Monoclonal Antibody (SPM507).

Package List Price ($)
$ 329