GFAP antibody

Cat. No. GTX100850
Cat. No.  GTX100850
Anti-GFAP antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100850
GTX100850 ICC/IF Image

GFAP antibody detects GFAP protein at glia cells by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min.
Green: GFAP protein stained by GFAP antibody (GTX100850) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, a neuron cell marker, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

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Anti-GFAP antibody used in Western Blot (WB). GTX100850
GTX100850 WB Image

Various whole cell and tissue extracts (5 and 30 μg) were separated by 10% SDS-PAGE, and the membranes were blotted with GFAP antibody (GTX100850) diluted at 1:2500 and competitor's antibody (CST 34001) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

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Anti-GFAP antibody used in Western Blot (WB). GTX100850
GTX100850 WB Image

Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GFAP antibody (GTX100850) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

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Anti-GFAP antibody used in IHC (Paraffin sections) (IHC-P). GTX100850
GTX100850 IHC-P Image

GFAP antibody detects GFAP protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
GFAP stained by GFAP antibody (GTX100850) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

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Anti-GFAP antibody used in IHC (Paraffin sections) (IHC-P). GTX100850
GTX100850 IHC-P Image

GFAP antibody detects GFAP protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
GFAP stained by GFAP antibody (GTX100850) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

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Anti-GFAP antibody used in IHC (Frozen sections) (IHC-Fr). GTX100850
GTX100850 IHC-Fr Image

GFAP antibody detects GFAP protein expression by immunohistochemical analysis.
Sample: Frozen-sectioned adult mouse hippocampus.
Green: GFAP protein stained by GFAP antibody (GTX100850) diluted at 1:250.
Red: NeuN, stained by NeuN antibody [2Q158] (GTX30773) diluted at 1:500.

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Anti-GFAP antibody used in IHC (Frozen sections) (IHC-Fr). GTX100850
GTX100850 IHC-Fr Image

GFAP antibody detects GFAP protein expression by immunohistochemical analysis.
Sample: Frozen-sectioned adult mouse cerebellum.
Green: GFAP protein stained by GFAP antibody (GTX100850) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

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Anti-GFAP antibody used in IHC (Frozen sections) (IHC-Fr). GTX100850
GTX100850 IHC-Fr Image

GFAP antibodies detects GFAP proteins on embryonic mouse brain by immunohistochemical analysis.
Sample: Frozen section of embryonic mouse brain (mE18.5).
Green: GFAP antibody (GTX100850) diluted at 1:500.
Red: Sox2 antibody [GT1876] (GTX627404) diluted at 1:500.

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Anti-GFAP antibody used in IHC (Frozen sections) (IHC-Fr). GTX100850
GTX100850 IHC-Fr Image

GFAP antibody detects GFAP protein on embryonic mouse brain by immunohistochemical analysis.
Sample:Frozen section of embryonic mouse brain (mE18.5).
Green: GFAP antibody (GTX100850) diluted at 1:500.
Blue: DAPI

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Anti-GFAP antibody used in IHC (Frozen sections) (IHC-Fr). GTX100850
GTX100850 IHC-Fr Image

GFAP antibody detects GFAP protein at retinal ganglion cell layer by immunohistochemical analysis.
Sample: Frozen sectioned adult mouse retina.
Green: GFAP protein stained by GFAP antibody (GTX100850) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).

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Anti-GFAP antibody used in Western Blot (WB). GTX100850
GTX100850 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GFAP antibody (GTX100850) diluted at 1:2500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

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Anti-GFAP antibody used in IHC (Frozen sections) (IHC-Fr). GTX100850
GTX100850 IHC-Fr Image

GFAP antibody detects GFAP protein expression by immunohistochemical analysis.
Sample: Frozen-sectioned adult mouse cerebellum.
Green: GFAP protein stained by GFAP antibody (GTX100850) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

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Anti-GFAP antibody used in IHC (Paraffin sections) (IHC-P). GTX100850
GTX100850 IHC-P Image

GFAP antibody detects GFAP protein at astrocyte on mouse fore brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse fore brain.
GFAP antibody (GTX100850) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

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Anti-GFAP antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100850
Anti-GFAP antibody used in Western Blot (WB). GTX100850
Anti-GFAP antibody used in Western Blot (WB). GTX100850
Anti-GFAP antibody used in IHC (Paraffin sections) (IHC-P). GTX100850
Anti-GFAP antibody used in IHC (Paraffin sections) (IHC-P). GTX100850
Anti-GFAP antibody used in IHC (Frozen sections) (IHC-Fr). GTX100850
Anti-GFAP antibody used in IHC (Frozen sections) (IHC-Fr). GTX100850
Anti-GFAP antibody used in IHC (Frozen sections) (IHC-Fr). GTX100850
Anti-GFAP antibody used in IHC (Frozen sections) (IHC-Fr). GTX100850
Anti-GFAP antibody used in IHC (Frozen sections) (IHC-Fr). GTX100850
Anti-GFAP antibody used in Western Blot (WB). GTX100850
Anti-GFAP antibody used in IHC (Frozen sections) (IHC-Fr). GTX100850
Anti-GFAP antibody used in IHC (Paraffin sections) (IHC-P). GTX100850
  • Host
    Rabbit
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Applications
    WB ICC/IF IHC-P IHC-Fr
  • Reactivity
    Human, Mouse, Rat
See all GFAP products