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GFAP antibody

Anti-GFAP antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100850
Anti-GFAP antibody used in IHC (Paraffin sections) (IHC-P). GTX100850
Anti-GFAP antibody used in IHC (Frozen sections) (IHC-Fr). GTX100850
Anti-GFAP antibody used in IHC (Paraffin sections) (IHC-P). GTX100850
Anti-GFAP antibody used in IHC (Frozen sections) (IHC-Fr). GTX100850
Anti-GFAP antibody used in IHC (Frozen sections) (IHC-Fr). GTX100850
Anti-GFAP antibody used in IHC (Frozen sections) (IHC-Fr). GTX100850
Anti-GFAP antibody used in IHC (Frozen sections) (IHC-Fr). GTX100850
Anti-GFAP antibody used in Western Blot (WB). GTX100850
Anti-GFAP antibody used in IHC (Paraffin sections) (IHC-P). GTX100850
Anti-GFAP antibody used in IHC (Frozen sections) (IHC-Fr). GTX100850
Anti-GFAP antibody used in Western Blot (WB). GTX100850
Anti-GFAP antibody used in Western Blot (WB). GTX100850

Cat No. GTX100850

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, IHC-Fr

Reactivity

Human, Mouse, Rat
Package
100 μl ($379),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
Not tested in other applications.

Calculated MW

50 kDa. ( Note )

Positive Control

GFAP-transfected 293T

Predict Reactivity

Bovine, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.72 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human GFAP. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10632188

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

glial fibrillary acidic protein , ALXDRD

Cellular Localization

Cytoplasm

Background

This gene encodes one of the major intermediate filament proteins of mature astrocytes. It is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-GFAP antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100850

GTX100850 ICC/IF Image

GFAP antibody detects GFAP protein at glia cells by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min.
Green: GFAP protein stained by GFAP antibody (GTX100850) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, a neuron cell marker, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-GFAP antibody used in IHC (Paraffin sections) (IHC-P). GTX100850

GTX100850 IHC-P Image

GFAP antibody detects GFAP protein expression in astrocytes/glia cells on mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
GFAP antibody (GTX100850) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-GFAP antibody used in IHC (Frozen sections) (IHC-Fr). GTX100850

GTX100850 IHC-Fr Image

GFAP antibody detects GFAP protein expression by immunohistochemical analysis.
Sample: Frozen-sectioned adult mouse cerebellum.
Green: GFAP protein stained by GFAP antibody (GTX100850) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-GFAP antibody used in IHC (Paraffin sections) (IHC-P). GTX100850

GTX100850 IHC-P Image

GFAP antibody detects GFAP protein expression in astrocytes/glia cells on rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
GFAP antibody (GTX100850) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-GFAP antibody used in IHC (Frozen sections) (IHC-Fr). GTX100850

GTX100850 IHC-Fr Image

GFAP antibody detects GFAP protein expression by immunohistochemical analysis.
Sample: Frozen-sectioned adult mouse hippocampus.
Green: GFAP protein stained by GFAP antibody (GTX100850) diluted at 1:250.
Red: NeuN, stained by NeuN antibody [2Q158] (GTX30773) diluted at 1:500.

Anti-GFAP antibody used in IHC (Frozen sections) (IHC-Fr). GTX100850

GTX100850 IHC-Fr Image

GFAP antibody detects GFAP protein at retinal ganglion cell layer by immunohistochemical analysis.
Sample: Frozen sectioned adult mouse retina.
Green: GFAP protein stained by GFAP antibody (GTX100850) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-GFAP antibody used in IHC (Frozen sections) (IHC-Fr). GTX100850

GTX100850 IHC-Fr Image

GFAP antibody detects GFAP protein on embryonic mouse brain by immunohistochemical analysis.
Sample:Frozen section of embryonic mouse brain (mE18.5).
Green: GFAP antibody (GTX100850) diluted at 1:500.
Blue: DAPI

Anti-GFAP antibody used in IHC (Frozen sections) (IHC-Fr). GTX100850

GTX100850 IHC-Fr Image

GFAP antibodies detects GFAP proteins on embryonic mouse brain by immunohistochemical analysis.
Sample: Frozen section of embryonic mouse brain (mE18.5).
Green: GFAP antibody (GTX100850) diluted at 1:500.
Red: Sox2 antibody [GT1876] (GTX627404) diluted at 1:500.

Anti-GFAP antibody used in Western Blot (WB). GTX100850

GTX100850 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GFAP antibody (GTX100850) diluted at 1:2500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-GFAP antibody used in IHC (Paraffin sections) (IHC-P). GTX100850

GTX100850 IHC-P Image

GFAP antibody detects GFAP protein at astrocyte on mouse fore brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse fore brain.
GFAP antibody (GTX100850) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-GFAP antibody used in IHC (Frozen sections) (IHC-Fr). GTX100850

GTX100850 IHC-Fr Image

GFAP antibody detects GFAP protein expression by immunohistochemical analysis.
Sample: Frozen-sectioned adult mouse cerebellum.
Green: GFAP protein stained by GFAP antibody (GTX100850) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-GFAP antibody used in Western Blot (WB). GTX100850

GTX100850 WB Image

Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GFAP antibody (GTX100850) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-GFAP antibody used in Western Blot (WB). GTX100850

GTX100850 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GFAP antibody (GTX100850) diluted at 1:2500.

REFERENCE

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 379
$ 169