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GFAP antibody

Anti-GFAP antibody used in IHC (Frozen sections) (IHC-Fr). GTX108711
Anti-GFAP antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX108711
Anti-GFAP antibody used in Western Blot (WB). GTX108711
Anti-GFAP antibody used in IHC (Paraffin sections) (IHC-P). GTX108711
Anti-GFAP antibody used in IHC (Paraffin sections) (IHC-P). GTX108711
Anti-GFAP antibody used in IHC (Frozen sections) (IHC-Fr). GTX108711
Anti-GFAP antibody used in IHC (Paraffin sections) (IHC-P). GTX108711
Anti-GFAP antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX108711
Anti-GFAP antibody used in IHC (Frozen sections) (IHC-Fr). GTX108711
Anti-GFAP antibody used in Western Blot (WB). GTX108711
Anti-GFAP antibody used in Western Blot (WB). GTX108711
Anti-GFAP antibody used in IHC (Paraffin sections) (IHC-P). GTX108711
Anti-GFAP antibody used in IHC (Frozen sections) (IHC-Fr). GTX108711
Anti-GFAP antibody used in IHC (Paraffin sections) (IHC-P). GTX108711

Cat. No. GTX108711

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, IHC-Fr, IHC

Reactivity

Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:5000-1:50000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
IHC Assay dependent
Not tested in other applications.

Calculated MW

50 kDa. ( Note )

Positive Control

GFAP-transfected 293T

Predict Reactivity

Bovine, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.47 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human GFAP. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2037091

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

glial fibrillary acidic protein , ALXDRD

Cellular Localization

Cytoplasm

Background

This gene encodes one of the major intermediate filament proteins of mature astrocytes. It is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-GFAP antibody used in IHC (Frozen sections) (IHC-Fr). GTX108711

GTX108711 IHC-Fr Image

GFAP antibody detects GFAP protein at retinal ganglion cell layer by immunohistochemical analysis.
Sample: Frozen sectioned adult mouse retina.
Green: GFAP protein stained by GFAP antibody (GTX108711) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-GFAP antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX108711

GTX108711 ICC/IF Image

GFAP antibody detects GFAP protein at glia cells by immunofluorescent analysis.
Sample: DIV10 rat E18 primary hippocampal neuron and glia cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: GFAP stained by GFAP antibody (GTX108711) diluted at 1:250.
Red: Tau, a cytoskeleton marker, stained by Tau antibody [GT287] (GTX634809) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-GFAP antibody used in Western Blot (WB). GTX108711

GTX108711 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GFAP antibody (GTX108711) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-GFAP antibody used in IHC (Paraffin sections) (IHC-P). GTX108711

GTX108711 IHC-P Image

GFAP antibody detects GFAP protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
GFAP stained by GFAP antibody (GTX108711) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-GFAP antibody used in IHC (Paraffin sections) (IHC-P). GTX108711

GTX108711 IHC-P Image

GFAP antibody detects GFAP protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
GFAP stained by GFAP antibody (GTX108711) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-GFAP antibody used in IHC (Frozen sections) (IHC-Fr). GTX108711

GTX108711 IHC-Fr Image

GFAP antibody detects GFAP protein expression by immunohistochemical analysis.
Sample: Frozen-sectioned adult mouse hippocampus.
Green: GFAP protein stained by GFAP antibody (GTX108711) diluted at 1:250.
Red: NeuN, stained by NeuN antibody [2Q158] (GTX30773) diluted at 1:500.

Anti-GFAP antibody used in IHC (Paraffin sections) (IHC-P). GTX108711

GTX108711 IHC-P Image

GFAP antibody detects GFAP protein at cytoplasm in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
Green: GFAP antibody (GTX108711) diluted at 1:200. The signal was developed using goat anti-rabbit IgG antibody (Dylight488) (GTX213110-04).
Blue: Nuclear staining with Hoechst 33342.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-GFAP antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX108711

GTX108711 ICC/IF Image

GFAP antibody detects GFAP protein at glia cells by immunofluorescent analysis.
Sample: DIV10 rat E18 primary cortical neuron and glia cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: GFAP stained by GFAP antibody (GTX108711) diluted at 1:500.
Red: Tau, stained by Tau antibody [GT287] (GTX634809) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-GFAP antibody used in IHC (Frozen sections) (IHC-Fr). GTX108711

GTX108711 IHC-Fr Image

GFAP antibody detects GFAP protein by immunohistochemical analysis.
Sample: Frozen-sectioned mouse spinal cord.
Green: GFAP stained by GFAP antibody (GTX108711) diluted at 1:300.
Blue: Hoechst 33342 staining.

Anti-GFAP antibody used in Western Blot (WB). GTX108711

GTX108711 WB Image

Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GFAP antibody (GTX108711) diluted at 1:25000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-GFAP antibody used in Western Blot (WB). GTX108711

GTX108711 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GFAP antibody (GTX108711) diluted at 1:2500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-GFAP antibody used in IHC (Paraffin sections) (IHC-P). GTX108711

GTX108711 IHC-P Image

GFAP antibody detects GFAP protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse hippocampus.
GFAP stained by GFAP antibody (GTX108711) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-GFAP antibody used in IHC (Frozen sections) (IHC-Fr). GTX108711

GTX108711 IHC-Fr Image

GFAP antibody detects GFAP protein expression by immunohistochemical analysis.
Sample: Frozen-sectioned adult mouse cerebellum.
Green: GFAP protein stained by GFAP antibody (GTX108711) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-GFAP antibody used in IHC (Paraffin sections) (IHC-P). GTX108711

GTX108711 IHC-P Image

GFAP antibody detects GFAP protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
GFAP stained by GFAP antibody (GTX108711) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

REVIEW

GFAP antibody Cat. No. GTX108711

Rating ( Average 5 based on 10 users reviews)
Application
Western Blot(WB) ( Average 5 based on 4 users reviews)
Immunocytochemistry/ Immunofluorescence(ICC/IF) ( Average 5 based on 3 users reviews)
IHC (Frozen sections)(IHC-Fr) ( Average 5 based on 3 users reviews)
Date : Anonymous submitted on 24-Jul-2023
Score :
Application Tested : ICC/IF
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the 2022 in Sci Rep. PMID: 35027586
Date : Anonymous submitted on 24-Jul-2023
Score :
Application Tested : ICC/IF
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the 2019 in Sci Rep. PMID: 30931957
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : IHC-Fr
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal Int J Mol Sci in 2020.PMID: 32098078
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal Cancer Med in 2019. PMID: 31274246
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal Cancer Med in 2019. PMID: 31274246
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal Cancer Med in 2019. PMID: 31274246
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal Cancer Med in 2019. PMID: 31274246
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : IHC-Fr
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal Cancer Med in 2019. PMID: 31274246
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : ICC/IF
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal Cancer Med in 2019. PMID: 31274246
Date : Anonymous submitted on 18-Oct-2011
Score :
Application Tested : IHC-Fr
Sample Species : Rat
Sample : S.D. rat brain sagittal cryo-section,50μm
Blocking : 1% BSA + 0.5% Troton X-100/PBS,pH=7.4, 25°C, 1Hr
Fixation : 4% PFA
Primary Antibody : 1:100, 4°C, 16Hr
Application Reference
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169