GeneTex
United States (US)

GFAP antibody

GTX108711_39981_20150625_IHC_M_19011507_264.jpg
GTX108711_39981_20170727_IFA_R_19011507_804.jpg
GTX108711_42564_20160830_IHC-Fr_19011507_808.jpg
GTX108711_42564_20161013_WB_M_R_19011507_708.jpg
GTX108711_42564_20160830_IHC-P_R_19011507_175.jpg
GTX108711_39981_20180727_WB_B_19011507_751.jpg
GTX108711_42564_20170531_IHC-Fr_M_19011507_381.jpg
GTX108711_43495_20190222_WB_19030801_958.jpg
GTX108711_42564_20170602_WB_19011507_810.jpg
GTX108711_39981_42886_IHC-Fr_M_19011507_203.jpg
GTX108711_39981_20150414_IHC-P_R_19011507_901.jpg

Cat No. GTX108711

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IHC-Fr
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:5000-1:50000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
Not tested in other applications.

Calculated MW

50 kDa. ( Note )

Positive Control

U87-MG , SK-N-SH , SK-N-AS , mouse brain , rat brain , GFAP-transfected 293T

Predict Reactivity

Bovine, Pig(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

2.83 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human GFAP. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Glial Fibrillary Acidic Protein,Alxdrd,Gfap

Cellular Localization

Cytoplasm

Background

This gene encodes one of the major intermediate filament proteins of mature astrocytes. It is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX108711_39981_20150625_IHC_M_19011507_264.jpg

GTX108711 IHC-Fr Image

GFAP antibodies detects GFAP proteins on embryonic mouse brain by immunohistochemical analysis.
Sample: Frozen section of embryonic mouse brain (mE18.5).
Green: GFAP antibody (GTX108711) diluted at 1:1000.
Red: Sox2 antibody [GT1352] (GTX627405) diluted at 1:500.

GTX108711_39981_20170727_IFA_R_19011507_804.jpg

GTX108711 ICC/IF Image

GFAP antibody detects GFAP protein at glia cells by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min.
Green: GFAP protein stained by GFAP antibody (GTX108711) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, a neuron cell marker, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

GTX108711_42564_20160830_IHC-Fr_19011507_808.jpg

GTX108711 IHC-Fr Image

GFAP antibody detects GFAP protein at retinal ganglion cell layer by immunohistochemical analysis.
Sample: Frozen sectioned adult mouse retina.
Green: GFAP protein stained by GFAP antibody (GTX108711) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).

GTX108711_42564_20161013_WB_M_R_19011507_708.jpg

GTX108711 WB Image

Various tissue extracts (50 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with GFAP antibody (GTX108711) diluted at 1:50000.

GTX108711_42564_20160830_IHC-P_R_19011507_175.jpg

GTX108711 IHC-P Image

GFAP antibody detects GFAP protein at cytoplasm in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
GFAP antibody (GTX108711) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX108711_39981_20180727_WB_B_19011507_751.jpg

GTX108711 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with GFAP antibody (GTX108711) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX108711_42564_20170531_IHC-Fr_M_19011507_381.jpg

GTX108711 IHC-Fr Image

GFAP antibody detects GFAP protein expression by immunohistochemical analysis.
Sample: Frozen-sectioned adult mouse cerebellum.
Green: GFAP protein stained by GFAP antibody (GTX108711) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

GTX108711_43495_20190222_WB_19030801_958.jpg

GTX108711 WB Image

Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with GFAP antibody (GTX108711) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX108711_42564_20170602_WB_19011507_810.jpg

GTX108711 WB Image

Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with GFAP antibody (GTX108711) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX108711_39981_42886_IHC-Fr_M_19011507_203.jpg

GTX108711 IHC-Fr Image

GFAP antibody detects GFAP protein expression by immunohistochemical analysis.
Sample: Frozen-sectioned adult mouse hippocampus.
Green: GFAP protein stained by GFAP antibody (GTX108711) diluted at 1:250.
Red: NeuN, stained by NeuN antibody [2Q158] (GTX30773) diluted at 1:500.

GTX108711_39981_20150414_IHC-P_R_19011507_901.jpg

GTX108711 IHC-P Image

GFAP antibody detects GFAP protein at cytoplasm in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
Green: GFAP antibody (GTX108711) diluted at 1:200. The signal was developed using goat anti-rabbit IgG antibody (Dylight488) (GTX213110-04).
Blue: Nuclear staining with Hoechst 33342.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE
REVIEW

GFAP antibody Cat No. GTX108711

Rating ( Average 5 based on 1 users reviews)
Application
IHC (Frozen sections)(IHC-Fr) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 18-Oct-2011
Score :
Application Tested : IHC-Fr
Sample Species : Rat
Sample : S.D. rat brain sagittal cryo-section,50μm
Blocking : 1% BSA + 0.5% Troton X-100/PBS,pH=7.4, 25°C, 1Hr
Fixation : 4% PFA
Primary Antibody : 1:100, 4°C, overnightHr
Application Reference
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169
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