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GFAP antibody

Anti-GFAP antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX108711
Anti-GFAP antibody used in IHC (Frozen sections) (IHC-Fr). GTX108711
Anti-GFAP antibody used in Western Blot (WB). GTX108711
Anti-GFAP antibody used in IHC (Frozen sections) (IHC-Fr). GTX108711
Anti-GFAP antibody used in Western Blot (WB). GTX108711
Anti-GFAP antibody used in Western Blot (WB). GTX108711
Anti-GFAP antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX108711
Anti-GFAP antibody used in IHC (Frozen sections) (IHC-Fr). GTX108711
Anti-GFAP antibody used in IHC (Paraffin sections) (IHC-P). GTX108711
Anti-GFAP antibody used in IHC (Paraffin sections) (IHC-P). GTX108711
Anti-GFAP antibody used in IHC (Paraffin sections) (IHC-P). GTX108711
Anti-GFAP antibody used in IHC (Frozen sections) (IHC-Fr). GTX108711

Cat No. GTX108711

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, IHC-Fr, IHC

Reactivity

Human, Mouse, Rat
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:5000-1:50000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
IHC Assay dependent
Not tested in other applications.

Calculated MW

50 kDa. ( Note )

Positive Control

U87-MG , SK-N-SH , SK-N-AS , mouse brain , rat brain , GFAP-transfected 293T

Predict Reactivity

Bovine, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.02 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human GFAP. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2037091

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

glial fibrillary acidic protein , ALXDRD

Cellular Localization

Cytoplasm

Background

This gene encodes one of the major intermediate filament proteins of mature astrocytes. It is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-GFAP antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX108711

GTX108711 ICC/IF Image

GFAP antibody detects GFAP protein at glia cells by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min.
Green: GFAP protein stained by GFAP antibody (GTX108711) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, a neuron cell marker, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-GFAP antibody used in IHC (Frozen sections) (IHC-Fr). GTX108711

GTX108711 IHC-Fr Image

GFAP antibody detects GFAP protein at retinal ganglion cell layer by immunohistochemical analysis.
Sample: Frozen sectioned adult mouse retina.
Green: GFAP protein stained by GFAP antibody (GTX108711) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-GFAP antibody used in Western Blot (WB). GTX108711

GTX108711 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GFAP antibody (GTX108711) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-GFAP antibody used in IHC (Frozen sections) (IHC-Fr). GTX108711

GTX108711 IHC-Fr Image

GFAP antibody detects GFAP protein expression by immunohistochemical analysis.
Sample: Frozen-sectioned adult mouse cerebellum.
Green: GFAP protein stained by GFAP antibody (GTX108711) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-GFAP antibody used in Western Blot (WB). GTX108711

GTX108711 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GFAP antibody (GTX108711) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-GFAP antibody used in Western Blot (WB). GTX108711

GTX108711 WB Image

Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GFAP antibody (GTX108711) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-GFAP antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX108711

GTX108711 ICC/IF Image

GFAP antibody detects GFAP protein at glia cells by immunofluorescent analysis.
Sample: DIV10 rat E18 primary cortical neuron and glia cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: GFAP stained by GFAP antibody (GTX108711) diluted at 1:500.
Red: Tau, stained by Tau antibody [GT287] (GTX634809) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-GFAP antibody used in IHC (Frozen sections) (IHC-Fr). GTX108711

GTX108711 IHC-Fr Image

GFAP antibody detects GFAP protein expression by immunohistochemical analysis.
Sample: Frozen-sectioned adult mouse hippocampus.
Green: GFAP protein stained by GFAP antibody (GTX108711) diluted at 1:250.
Red: NeuN, stained by NeuN antibody [2Q158] (GTX30773) diluted at 1:500.

Anti-GFAP antibody used in IHC (Paraffin sections) (IHC-P). GTX108711

GTX108711 IHC-P Image

GFAP antibody detects GFAP protein at cytoplasm in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
Green: GFAP antibody (GTX108711) diluted at 1:200. The signal was developed using goat anti-rabbit IgG antibody (Dylight488) (GTX213110-04).
Blue: Nuclear staining with Hoechst 33342.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-GFAP antibody used in IHC (Paraffin sections) (IHC-P). GTX108711

GTX108711 IHC-P Image

GFAP antibody detects GFAP protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
GFAP stained by GFAP antibody (GTX108711) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-GFAP antibody used in IHC (Paraffin sections) (IHC-P). GTX108711

GTX108711 IHC-P Image

GFAP antibody detects GFAP protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse hippocampus.
GFAP stained by GFAP antibody (GTX108711) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-GFAP antibody used in IHC (Frozen sections) (IHC-Fr). GTX108711

GTX108711 IHC-Fr Image

GFAP antibody detects GFAP protein by immunohistochemical analysis.
Sample: Frozen-sectioned mouse spinal cord.
Green: GFAP stained by GFAP antibody (GTX108711) diluted at 1:300.
Blue: Hoechst 33342 staining.

REFERENCE

REVIEW

GFAP antibody Cat No. GTX108711

Rating ( Average 5 based on 7 users reviews)
Application
Western Blot(WB) ( Average 5 based on 4 users reviews)
Immunocytochemistry/ Immunofluorescence(ICC/IF) ( Average 5 based on 1 users reviews)
IHC (Frozen sections)(IHC-Fr) ( Average 5 based on 2 users reviews)
Date : Anonymous submitted on 23-Dec-2020
Score :
Application Tested : WB
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal Cancer Med in 2019. PMID: 31274246
Date : Anonymous submitted on 23-Dec-2020
Score :
Application Tested : IHC-Fr
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal Cancer Med in 2019. PMID: 31274246
Date : Anonymous submitted on 23-Dec-2020
Score :
Application Tested : ICC/IF
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal Cancer Med in 2019. PMID: 31274246
Date : Anonymous submitted on 23-Dec-2020
Score :
Application Tested : WB
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal Cancer Med in 2019. PMID: 31274246
Date : Anonymous submitted on 23-Dec-2020
Score :
Application Tested : WB
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal Cancer Med in 2019. PMID: 31274246
Date : Anonymous submitted on 23-Dec-2020
Score :
Application Tested : WB
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal Cancer Med in 2019. PMID: 31274246
Date : Anonymous submitted on 18-Oct-2011
Score :
Application Tested : IHC-Fr
Sample Species : Rat
Sample : S.D. rat brain sagittal cryo-section,50μm
Blocking : 1% BSA + 0.5% Troton X-100/PBS,pH=7.4, 25°C, 1Hr
Fixation : 4% PFA
Primary Antibody : 1:100, 4°C, 16Hr
Application Reference
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 329
$ 169