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GFAP antibody

Anti-GFAP antibody used in Immunohistochemistry (IHC). GTX85454
Anti-GFAP antibody used in Immunohistochemistry (IHC). GTX85454

Cat. No. GTX85454

Reference 12

Host

 Chicken

Clonality

 Polyclonal

Isotype

 IgY

Application

 WB, ICC/IF, IHC-P, IHC-Fr, IHC

Reactivity

 Human, Mouse, Rat
Package
50 μg ($399)
See all GFAP products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:2000
ICC/IF Assay dependent
IHC-P Assay dependent
IHC-Fr Assay dependent
IHC 1:500-1:1000
Not tested in other applications.

Calculated MW

50 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.

Concentration

2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Chickens were immunized with recombinant GFAP (expressed in bacteria).

Purification

IgY fraction

Conjugation

Unconjugated

RRID

AB_10621124

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

glial fibrillary acidic protein , ALXDRD

Cellular Localization

Cytoplasm

Background

This gene encodes one of the major intermediate filament proteins of mature astrocytes. It is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Oct 2008]

Database

Research Area

DATA IMAGES

Anti-GFAP antibody used in Immunohistochemistry (IHC). GTX85454

GTX85454 IHC Image

IHC analysis of E16 mouse brain using GTX85454 GFAP antibody. Section was a vibratome, thick section (20 um) using a lightly-fixed (2% paraformaldehyde) mouse brain.

Anti-GFAP antibody used in Immunohistochemistry (IHC). GTX85454

GTX85454 IHC Image

IHC analysis of mouse brain cultures using GTX85454 GFAP antibody.
Red : Primary antibody
Blue : DAPI

REFERENCE

Application Reference

H Wei et al. J Neuroinflammation 2023; 20 (1) : 264 Glyceryl triacetate promotes blood–brain barrier recovery after ischemic stroke through lipogenesis-mediated IL-33 in mice
Application : ICC/IF, IHC-Fr
S Zhu et al. Research Square 2023; Trimethylamine N-oxide / SREBP2 pathway is involved in sleep deprivation-induced cognitive dysfunction through regulating astrocytic cholesterol metabolism
Application : ICC/IF, IHC-Fr
Reactivity : Mouse
Li Q et al. Autophagy 2023; 19 (10) : 2639-2656 Impaired lipophagy induced-microglial lipid droplets accumulation contributes to the buildup of TREM1 in diabetes-associated cognitive impairment.
Jiang T et al. EBioMedicine 2023; 93 : 104653 Reprogramming astrocytic NDRG2/NF-κB/C3 signaling restores the diabetes-associated cognitive dysfunction.
Juras JA et al. Cell Rep Methods 2023; 3 (4) : 100455 In situ microwave fixation provides an instantaneous snapshot of the brain metabolome.
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REVIEW

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Application Reference
H Wei et al. J Neuroinflammation 2023; 20 (1):264 Glyceryl triacetate promotes blood–brain barrier recovery after ischemic stroke through lipogenesis-mediated IL-33 in mice
Application : ICC/IF, IHC-Fr
S Zhu et al. Research Square 2023; Trimethylamine N-oxide / SREBP2 pathway is involved in sleep deprivation-induced cognitive dysfunction through regulating astrocytic cholesterol metabolism
Application : ICC/IF, IHC-Fr
Reactivity : Mouse
Li Q et al. Autophagy 2023; 19 (10):2639-2656 Impaired lipophagy induced-microglial lipid droplets accumulation contributes to the buildup of TREM1 in diabetes-associated cognitive impairment.
Jiang T et al. EBioMedicine 2023; 93:104653 Reprogramming astrocytic NDRG2/NF-κB/C3 signaling restores the diabetes-associated cognitive dysfunction.
Juras JA et al. Cell Rep Methods 2023; 3 (4):100455 In situ microwave fixation provides an instantaneous snapshot of the brain metabolome.
Xiaofeng G et al. CNS Neurosci Ther 2023; 29 (4):1178-1191 PERK-STING-RIPK3 pathway facilitates cognitive impairment by inducing neuronal necroptosis in sepsis-associated encephalopathy.
Reactivity : Mouse
Huang WY et al. J Neuroinflammation 2022; 19 (1):29 TNFα-mediated necroptosis in brain endothelial cells as a potential mechanism of increased seizure susceptibility in mice following systemic inflammation.
Application : IHC-Fr
Reactivity : Mouse
Kremer LPM et al. Mol Ther Methods Clin Dev 2021; 23:33-50 High throughput screening of novel AAV capsids identifies variants for transduction of adult NSCs within the subventricular zone.
Application : IHC
Reactivity : Mouse
Gao H et al. Mol Neurobiol 2021; μ-Opioid Receptor-Mediated Enteric Glial Activation Is Involved in Morphine-Induced Constipation.
Zhang Y et al. J Mol Histol 2021; 52 (1):101-111 NDRG2 is expressed on enteric glia and altered in conditions of inflammation and oxygen glucose deprivation/reoxygenation.
Application : WB
Reactivity : Mouse
Fan Z et al. Neurosci Bull 2020; 36 (12):1513-1523 Dynamic Variations in Brain Glycogen are Involved in Modulating Isoflurane Anesthesia in Mice.
Application : IHC-P
Reactivity : Mouse
Huang WY et al. J Neuroinflammation 2018; 15 (1):140 NADPH oxidases as potential pharmacological targets against increased seizure susceptibility after systemic inflammation.
Application : WB
Reactivity : Mouse
SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399