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GFM2 antibody [N3C2], Internal

Anti-GFM2 antibody [N3C2], Internal used in Western Blot (WB). GTX122890
Anti-GFM2 antibody [N3C2], Internal used in Western Blot (WB). GTX122890

Cat. No. GTX122890

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all GFM2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

87 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , Neuro 2A , C8D30

Predict Reactivity

Rat, Bovine, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human GFM2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11175487

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

G elongation factor mitochondrial 2 , EF-G2mt , EFG2 , MRRF2 , MST027 , MSTP027 , RRF2 , RRF2mt , hEFG2 , mEF-G 2

Cellular Localization

Mitochondrion

Background

Eukaryotes contain two protein translational systems, one in the cytoplasm and one in the mitochondria. Mitochondrial translation is crucial for maintaining mitochondrial function and mutations in this system lead to a breakdown in the respiratory chain-oxidative phosphorylation system and to impaired maintenance of mitochondrial DNA. This gene encodes one of mitochondrial translation elongation factors. Its role in the regulation of normal mitochondrial function and in different disease states attributed to mitochondrial dysfunction is not known. Alternative splicing results in at least three transcript variants encoding distinct isoforms. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-GFM2 antibody [N3C2], Internal used in Western Blot (WB). GTX122890

GTX122890 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GFM2 antibody [N3C2], Internal (GTX122890) diluted at 1:500.

Anti-GFM2 antibody [N3C2], Internal used in Western Blot (WB). GTX122890

GTX122890 WB Image

GFM2 antibody detects GFM2 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GFM2 antibody (GTX122890) diluted at 1:2000.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169