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GFM2 antibody

Anti-GFM2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX124071
Anti-GFM2 antibody used in Western Blot (WB). GTX124071
Anti-GFM2 antibody used in IHC (Paraffin sections) (IHC-P). GTX124071

Cat No. GTX124071

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Mouse
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

87 kDa. ( Note )

Positive Control

293T , HepG2

Predict Reactivity

Rat, Bovine, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.62 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human GFM2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

G elongation factor mitochondrial 2 , EF-G2mt , EFG2 , MRRF2 , MST027 , MSTP027 , RRF2 , RRF2mt , hEFG2 , mEF-G 2

Cellular Localization

Mitochondrion

Background

Eukaryotes contain two protein translational systems, one in the cytoplasm and one in the mitochondria. Mitochondrial translation is crucial for maintaining mitochondrial function and mutations in this system lead to a breakdown in the respiratory chain-oxidative phosphorylation system and to impaired maintenance of mitochondrial DNA. This gene encodes one of mitochondrial translation elongation factors. Its role in the regulation of normal mitochondrial function and in different disease states attributed to mitochondrial dysfunction is not known. Alternative splicing results in at least three transcript variants encoding distinct isoforms. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-GFM2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX124071

GTX124071 ICC/IF Image

GFM2 antibody detects GFM2 protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in 2% paraformaldehyde/culture medium at 37ºC for 30 min.
Green: GFM2 protein stained by GFM2 antibody (GTX124071) diluted at 1:500.
Blue: Hoechst 33342 staining.

Anti-GFM2 antibody used in Western Blot (WB). GTX124071

GTX124071 WB Image

GFM2 antibody detects GFM2 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GFM2 antibody (GTX124071) diluted at a dilution of 1:500.

Anti-GFM2 antibody used in IHC (Paraffin sections) (IHC-P). GTX124071

GTX124071 IHC-P Image

GFM2 antibody detects GFM2 protein at cytosol on mouse testis by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
GFM2 antibody (GTX124071) dilution: 1:1000.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169