APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Dilution |
| 1:500-1:3000 |
| 1:100-1:1000 |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
293T , HepG2
Predict Reactivity
Rat, Bovine, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.62 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human GFM2. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_2885594
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
G elongation factor mitochondrial 2 , EF-G2mt , EFG2 , MRRF2 , MST027 , MSTP027 , RRF2 , RRF2mt , hEFG2 , mEF-G 2
Cellular Localization
Mitochondrion
Background
Eukaryotes contain two protein translational systems, one in the cytoplasm and one in the mitochondria. Mitochondrial translation is crucial for maintaining mitochondrial function and mutations in this system lead to a breakdown in the respiratory chain-oxidative phosphorylation system and to impaired maintenance of mitochondrial DNA. This gene encodes one of mitochondrial translation elongation factors. Its role in the regulation of normal mitochondrial function and in different disease states attributed to mitochondrial dysfunction is not known. Alternative splicing results in at least three transcript variants encoding distinct isoforms. [provided by RefSeq]
Database
Research Area
DATA IMAGES
|
GTX124071 ICC/IF Image
GFM2 antibody detects GFM2 protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in 2% paraformaldehyde/culture medium at 37ºC for 30 min.
Green: GFM2 protein stained by GFM2 antibody (GTX124071) diluted at 1:500.
Blue: Hoechst 33342 staining.
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GTX124071 WB Image
GFM2 antibody detects GFM2 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GFM2 antibody (GTX124071) diluted at a dilution of 1:500.
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GTX124071 IHC-P Image
GFM2 antibody detects GFM2 protein at cytosol on mouse testis by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
GFM2 antibody (GTX124071) dilution: 1:1000.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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REFERENCE
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REVIEW
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![Anti-Citrate synthase antibody [GT1761] used in Western Blot (WB). GTX628143](/upload/website/prouct_img/small/GTX628143/GTX628143_41113_20191129_WB_Fraction_19120122_515.jpg "Anti-Citrate synthase antibody [GT1761] used in Western Blot (WB). GTX628143")
