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GFM2 antibody

Anti-GFM2 antibody used in Western Blot (WB). GTX87245
Anti-GFM2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX87245

Cat. No. GTX87245

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human, Mouse
Package
100 μg ($399)
See all GFM2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500~1:1000
ICC/IF 1:100~1:500
Not tested in other applications.

Calculated MW

87 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH7.4, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The antiserum was produced against synthesized peptide derived from human GFM2 (441-490).

Purification

Purified by antigen-affinity chromatography
From serum

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

EFG2 , EFG2mt , G elongation factor mitochondrial 2 , GFM2 , MRRF2 , MST027 , MSTP027 , RRF2 , RRF2mt , hEFG2 , mEFG 2 , G elongation factor, mitochondrial 2

Cellular Localization

Mitochondrion

Background

Eukaryotes contain two protein translational systems, one in the cytoplasm and one in the mitochondria. Mitochondrial translation is crucial for maintaining mitochondrial function and mutations in this system lead to a breakdown in the respiratory chain-oxidative phosphorylation system and to impaired maintenance of mitochondrial DNA. This gene encodes one of the mitochondrial translation elongation factors, which is a GTPase that plays a role at the termination of mitochondrial translation by mediating the disassembly of ribosomes from messenger RNA . Its role in the regulation of normal mitochondrial function and in disease states attributed to mitochondrial dysfunction is not known. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2013]

Database

Research Area

DATA IMAGES

Anti-GFM2 antibody used in Western Blot (WB). GTX87245

GTX87245 WB Image

WB analysis of LOVO and RAW264.7 cell lysates using GTX87245 GFM2 antibody. The lane on the right is blocked with the synthesized peptide.

Anti-GFM2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX87245

GTX87245 ICC/IF Image

ICC/IF analysis of A549 cells using GTX87245 GFM2 antibody. The picture on the right is blocked with the synthesized peptide.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399