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GFP antibody [1A5]

Anti-GFP antibody [1A5] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00694
Anti-GFP antibody [1A5] used in Western Blot (WB). GTX00694
Anti-GFP antibody [1A5] used in Immunoprecipitation (IP). GTX00694

Cat. No. GTX00694

Reference 2

Host

 Rat

Clonality

 Monoclonal

Clone Name

 1A5

Isotype

 IgG1

Application

 WB, ICC/IF, IP, ELISA, ChIP assay

Reactivity

 Species independent
Package
100 μg ($399)
See all GFP products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB ~1 μg/ml
ICC/IF Assay dependent
IP Assay dependent
ELISA Assay dependent
ChIP assay Assay dependent
Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

Filter-sterilized PBS, 50% Glycerol

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

Recombinant GFP protein

Purification

Purified IgG

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Research Area

DATA IMAGES

Anti-GFP antibody [1A5] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00694

GTX00694 ICC/IF Image

ICC/IF analysis of COS1 cell lysate overexpressed GFP and GST tagged ZIPK fusion protein using GTX00694 GFP antibody [1A5].
Note that fluorescence by the immunofluorescent staining using 1A5 antibody is much stronger than fluorescence of GFP.
Red : Primary antibody
Green : Fluorescence of GFP

Anti-GFP antibody [1A5] used in Western Blot (WB). GTX00694

GTX00694 WB Image

WB analysis of various samples using GTX00694 GFP antibody [1A5].
(-) : HEK293T cell lysate
(+) : HEK293T cell lysate overexpressed GFP-ZIPK protein

Anti-GFP antibody [1A5] used in Immunoprecipitation (IP). GTX00694

GTX00694 IP Image

IP analysis of GST-GFP fusion protein using GTX00694 GFP antibody [1A5].

REFERENCE

Clone Reference

Maehara K et al. Epigenetics Chromatin. 2015; Tissue-specific expression of histone H3 variants diversified after species separation.
Okazaki K et al. Carcinogenesis 2012; 33 (10):1854-62 Nuclear localization signal in a cancer-related transcriptional regulator protein NAC1.
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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Package List Price ($)
$ 399