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GFP antibody (Biotin)

Anti-GFP antibody (Biotin) used in Western Blot (WB). GTX26658

Cat No. GTX26658

Host

Goat

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, ELISA, IHC
Package
100 μg ($339)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:2000 - 1:10000
ICC/IF Assay dependent
IHC-P Assay dependent
ELISA 1:50000 - 1:80000
IHC 1:1000 - 1:5000
Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 and 0.01% (w/v) Sodium Azide and 10 mg/mL Bovine Serum Albumin (BSA)

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

Recombinant Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246aa) derived from the jellyfish Aequorea victoria.

Purification

Purified by antigen-affinity chromatography.
From polyclonal serum.

Conjugation

Biotin
Ratio : 10-20 Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC) molecules per Goat IgG molecule.

RRID

AB_371422

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Background

Designed to detect GFP and its variants in ELISA (sandwich or capture), immunoblotting and immunoprecipitation

Research Area

DATA IMAGES

Anti-GFP antibody (Biotin) used in Western Blot (WB). GTX26658

GTX26658 WB Image

Western blot of Biotin Goat Anti-GFP antibody. Lane 1: GFP. Load: 50 ng per lane. Primary antibody: GFP antibody Biotin conjugated at 1:1,000 for 60 min at RT. Secondary antibody: Peroxidase streptavidin secondary antibody at 1:40,000 for 30 min at RT. Blocking for 30 min at RT. Predicted/Observed size: 28 kDa, 28 kDa for GFP. Other band(s): None.

REFERENCE

REVIEW

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Application Reference
SDS
Sodium Azide.pdf
Package List Price ($)
$ 339