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GFP antibody (Biotin)

Anti-GFP antibody (Biotin) used in Western Blot (WB). GTX26658

Cat. No. GTX26658

Reference 12

Host

 Goat

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, ELISA, IHC
Package
100 μg ($399)
See all GFP products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:2000 - 1:10000
ICC/IF Assay dependent
IHC-P Assay dependent
ELISA 1:50000 - 1:80000
IHC 1:1000 - 1:5000
Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

0.02M Potassium Phosphate pH7.2, 0.15M Sodium Chloride, 10mg/ml BSA

Preservative

0.01% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

Recombinant Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246aa) derived from the jellyfish Aequorea victoria.

Purification

Purified by antigen-affinity chromatography.
From polyclonal serum.

Conjugation

Biotin
Ratio : 10-20 Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC) molecules per Goat IgG molecule.

RRID

AB_371422

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Background

Designed to detect GFP and its variants in ELISA (sandwich or capture), immunoblotting and immunoprecipitation

Research Area

DATA IMAGES

Anti-GFP antibody (Biotin) used in Western Blot (WB). GTX26658

GTX26658 WB Image

Western blot of Biotin Goat Anti-GFP antibody. Lane 1: GFP. Load: 50 ng per lane. Primary antibody: GFP antibody Biotin conjugated at 1:1,000 for 60 min at RT. Secondary antibody: Peroxidase streptavidin secondary antibody at 1:40,000 for 30 min at RT. Blocking for 30 min at RT. Predicted/Observed size: 28 kDa, 28 kDa for GFP. Other band(s): None.

REFERENCE

Application Reference

Bettke JA et al. Infect Immun 2022; 90 (4) : e0007022 Inflammatory Monocytes Promote Granuloma-Mediated Control of Persistent Salmonella Infection.
Application : IHC-P
Franziska A Graef et al. PLoS Pathog 2021; 17 (8) : e1009719 Fasting increases microbiome-based colonization resistance and reduces host inflammatory responses during an enteric bacterial infection.
Application : IHC-P
Manko-Prykhoda A et al. Int J Parasitol 2020; 50 (4) : 263-275 Giardia spp. promote the production of antimicrobial peptides and attenuate disease severity induced by attaching and effacing enteropathogens via the induction of the NLRP3 inflammasome.
Application : IHC-P
Schmitt M et al. Cell Rep 2018; 24 (9) : 2312-2328.e7 Paneth Cells Respond to Inflammation and Contribute to Tissue Regeneration by Acquiring Stem-like Features through SCF/c-Kit Signaling.
Application : WB
Dunlap MD et al. Mucosal Immunol 2018; (Epub) A novel role for C-C motif chemokine receptor 2 during infection with hypervirulent Mycobacterium tuberculosis.
Application : IHC-P
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REVIEW

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Application Reference
Bettke JA et al. Infect Immun 2022; 90 (4):e0007022 Inflammatory Monocytes Promote Granuloma-Mediated Control of Persistent Salmonella Infection.
Application : IHC-P
Franziska A Graef et al. PLoS Pathog 2021; 17 (8):e1009719 Fasting increases microbiome-based colonization resistance and reduces host inflammatory responses during an enteric bacterial infection.
Application : IHC-P
Manko-Prykhoda A et al. Int J Parasitol 2020; 50 (4):263-275 Giardia spp. promote the production of antimicrobial peptides and attenuate disease severity induced by attaching and effacing enteropathogens via the induction of the NLRP3 inflammasome.
Application : IHC-P
Schmitt M et al. Cell Rep 2018; 24 (9):2312-2328.e7 Paneth Cells Respond to Inflammation and Contribute to Tissue Regeneration by Acquiring Stem-like Features through SCF/c-Kit Signaling.
Application : WB
Dunlap MD et al. Mucosal Immunol 2018; (Epub) A novel role for C-C motif chemokine receptor 2 during infection with hypervirulent Mycobacterium tuberculosis.
Application : IHC-P
Houbracken I et al. Sci Rep 2017; 7 (1):12643 Acinar cells in the neonatal pancreas grow by self-duplication and not by neogenesis from duct cells.
Manko A et al. PLoS One 2017; 12 (6):e0178647 Giardia co-infection promotes the secretion of antimicrobial peptides beta-defensin 2 and trefoil factor 3 and attenuates attaching and effacing bacteria-induced intestinal disease.
Application : IHC-P
Paratore F et al. Anal Chem 2017; (Epub) Isotachophoresis-Based Surface Immunoassay.
Morampudi V et al. Infect Immun 2016; (Epub) Tricellular Tight Junction Protein Tricellulin is targeted by the Enteropathogenic E. coli Effector EspG1 Leading to Epithelial Barrier Disruption.
Application : ICC/IF
Song I et al. PLoS One 2015; 10 (10):e0140148 Beta Cell Mass Restoration in Alloxan-Diabetic Mice Treated with EGF and Gastrin.
Burger NB et al. Prenat Diagn 2014; Involvement of neurons and retinoic acid in lymphatic development: new insights in increased nuchal translucency.
Bergstrom KS et al. PLoS Pathog 2010; 6 (5):e1000902 Muc2 protects against lethal infectious colitis by disassociating pathogenic and commensal bacteria from the colonic mucosa.
Application : IHC-P
SDS
Sodium Azide.pdf
Package List Price ($)
$ 399