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GFP antibody (FITC)

Anti-GFP antibody (FITC) used in Western Blot (WB). GTX26662
Anti-GFP antibody (FITC) used in IHC (Frozen sections) (IHC-Fr). GTX26662

Cat. No. GTX26662

Reference 17
5

Host

 Goat

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-Fr, IHC-Wm, ELISA, IHC
Package
100 μg ($399)
See all GFP products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB >1:10000
ICC/IF 1:500 - 1:2500
IHC-Fr Assay dependent
IHC-Wm Assay dependent
ELISA >1:20000
IHC Assay dependent
Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

0.02M Potassium Phosphate pH7.2, 0.15M Sodium Chloride, 10mg/ml BSA

Preservative

0.01% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. Protect from light.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

The immunogen is a Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246aa) derived from the jellyfish Aequorea victoria.

Purification

Purified by antigen-affinity chromatography.
From polyclonal serum

Conjugation

Fluorescein isothiocyanate (FITC)
Absorption : 495 nm ; Emission : 528 nm. Ratio : 3.5 molecules FITC per Goat IgG molecule.

RRID

AB_371424

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Background

The jellyfish Aequorea victoria contains green fluorescent protein (GFP) that emits light in the bioluminescence reaction of the animal. GFP has been used widely as a reporter protein for gene expression in eukaryotic and prokaryotic organisms, and as a protein tag in cell culture and in multicellular organisms. As a fusion tag, GFP can be used to localize proteins, to study their movement or to research the dynamics of the subcellular compartments where these proteins are targeted. GFP technology has revealed considerable new insights into the physiological activities of living cells.

Research Area

DATA IMAGES

Anti-GFP antibody (FITC) used in Western Blot (WB). GTX26662

GTX26662 WB Image

Western Blot of Goat anti-GFP Fluorescein Conjugated Antibody (GTX26662). Lane 1: GFP. Load: 50 ng per lane. Primary antibody: None. Secondary antibody: Fluorescein goat secondary antibody at 1:1,000 for 60 min at RT. Block for 30 min at RT. Predicted/Observed size: 28 kDa, 33 kDa for GFP. Other band(s): None.

Anti-GFP antibody (FITC) used in IHC (Frozen sections) (IHC-Fr). GTX26662

GTX26662 IHC-Fr Image

Immunofluorescence Microscopy of GFP-GOAT-Antibody. Tissue: Sf-1:Cre mice crossed to the Z/EG reporter line. Mouse brain (coronal view, 20X magnification). Fixation: 4%PFA/PBS with o/n fixation, and subsequently transferred to a 30% sucrose solution. Antigen retrieval: frozen in OCT freezing medium (Sakura) and cryostat sectioned at 40 microns. Primary antibody: Goat anti-GFP (GTX26662) was used at 1:500 dilution in free floating imunnohistochemistry to detect GFP. Secondary antibody: Fluorchrome conjugated Anti-goat IgG secondary antibody was used for detection at 1:500 at 1:10,000 for 45 min at RT. Localization: Sf-1+ neurons and their processes of the ventromedial nucleus of the hypothalamus. Staining: eGFP as green fluorescent signal and sections were counterstained with DAPI.

REFERENCE

Application Reference

Evsen L et al. Development 2020; 147 (15) let-7 miRNAs inhibit CHD7 expression and control auditory-sensory progenitor cell behavior in the developing inner ear.
Laiouar S et al. Curr Biol 2020; 30 (7) : 1177-1188.e5 RabX1 Organizes a Late Endosomal Compartment that Forms Tubular Connections to Lysosomes Consistent with a "Kiss and Run" Mechanism.
Lale Evsen et al. bioRxiv 2019; (Epub) Let-7 miRNAs control auditory sensory progenitor behavior in the vertebrate inner ear.
Application : IHC-Fr
Clayton ZE et al. Biosci Rep 2018; (Epub) Induced pluripotent stem cell-derived endothelial cells promote angiogenesis and accelerate wound closure in a murine excisional wound healing model.
Application : IHC
Chan AH et al. PLoS One 2017; 12 (3) : e0174773 Evaluation of synthetic vascular grafts in a mouse carotid grafting model.
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REVIEW

GFP antibody (FITC) Cat. No. GTX26662

Rating
( Average 5 based on 5 users reviews)
Application
IHC (Whole mount)(IHC-Wm)
( Average 5 based on 1 users reviews)
Immunohistochemistry(IHC)
( Average 5 based on 4 users reviews)
Anti-GFP antibody (FITC) used in IHC (Whole mount) (IHC-Wm). GTX26662
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : IHC-Wm
Figure Note : The data was peer reviewed and published in the journal PLoS Genet in 2008.PMID: 18404215
Anti-GFP antibody (FITC) used in Immunohistochemistry (IHC). GTX26662
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : IHC
Figure Note : The data was peer reviewed and published in the journal Nat Commun in 2016. PMID: 26960923
Anti-GFP antibody (FITC) used in Immunohistochemistry (IHC). GTX26662
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : IHC
Figure Note : The data was peer reviewed and published in the journal Nat Commun in 2016. PMID: 26960923
Anti-GFP antibody (FITC) used in Immunohistochemistry (IHC). GTX26662
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : IHC
Figure Note : The data was peer reviewed and published in the journal Nat Commun in 2016. PMID: 26960923
Anti-GFP antibody (FITC) used in Immunohistochemistry (IHC). GTX26662
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : IHC
Figure Note : The data was peer reviewed and published in the journal Nat Commun in 2016. PMID: 26960923
Submit a Review
Application Reference
Evsen L et al. Development 2020; 147 (15) let-7 miRNAs inhibit CHD7 expression and control auditory-sensory progenitor cell behavior in the developing inner ear.
Laiouar S et al. Curr Biol 2020; 30 (7):1177-1188.e5 RabX1 Organizes a Late Endosomal Compartment that Forms Tubular Connections to Lysosomes Consistent with a "Kiss and Run" Mechanism.
Lale Evsen et al. bioRxiv 2019; (Epub) Let-7 miRNAs control auditory sensory progenitor behavior in the vertebrate inner ear.
Application : IHC-Fr
Clayton ZE et al. Biosci Rep 2018; (Epub) Induced pluripotent stem cell-derived endothelial cells promote angiogenesis and accelerate wound closure in a murine excisional wound healing model.
Application : IHC
Chan AH et al. PLoS One 2017; 12 (3):e0174773 Evaluation of synthetic vascular grafts in a mouse carotid grafting model.
Woichansky I et al. Nat Commun 2016; 7:10834 Three mechanisms control E-cadherin localization to the zonula adherens.
Application : IHC
Raft S et al. Dev Biol 2014; 390 (1):51-67 Ephrin-B2 governs morphogenesis of endolymphatic sac and duct epithelia in the mouse inner ear.
Raft S et al. PLoS One 2014; 9 (10):e109043 Pou3f4-mediated regulation of ephrin-b2 controls temporal bone development in the mouse.
Application : ICC/IF
de Renty C et al. PLoS One 2014; 9 (5):e97434 Cytoplasmic Localization of p21 Protects Trophoblast Giant Cells from DNA Damage Induced Apoptosis.
Application : IHC-Wm
Berns N et al. J Cell Sci 2014; A genome-scale in vivo RNAi analysis of epithelial development in Drosophila identifies new proliferation domains outside of the stem cell niche.
Application : ICC/IF
Li X et al. PLoS Genet 2013; 9 (7):e1003641 SLC26A4 targeted to the endolymphatic sac rescues hearing and balance in Slc26a4 mutant mice.
Evsen L et al. J Neurosci 2013; 33 (9):3879-90 Progression of neurogenesis in the inner ear requires inhibition of Sox2 transcription by neurogenin1 and neurod1.
Application : IHC
Gomez JM et al. J Cell Biol 2012; 199 (7):1131-43 Tao controls epithelial morphogenesis by promoting Fasciclin 2 endocytosis.
Klausner EA et al. J Gene Med 2012; 14 (2):100-8 Corneal gene delivery: chitosan oligomer as a carrier of CpG rich, CpG free or S/MAR plasmid DNA.
Mercapide J et al. Int J Cancer 2010; 126 (5):1206-15 Primary gene-engineered neural stem/progenitor cells demonstrate tumor-selective migration and antitumor effects in glioma.
Hwang CH et al. Dev Dyn 2009; 238 (11):2725-34 Foxg1 is required for proper separation and formation of sensory cristae during inner ear development.
Application : IHC-Wm
Chang W et al. PLoS Genet 2008; 4 (4):e1000050 Bmp4 is essential for the formation of the vestibular apparatus that detects angular head movements.
SDS
Sodium Azide.pdf
Package List Price ($)
$ 399