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GFP antibody (FITC)

Anti-GFP antibody (FITC) used in IHC (Frozen sections) (IHC-Fr). GTX26662
Anti-GFP antibody (FITC) used in Western Blot (WB). GTX26662

Cat No. GTX26662

Host

Goat

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-Fr, IHC-Wm, ELISA, IHC
Package
100 μg ($339)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB >1:10000
ICC/IF 1:500 - 1:2500
IHC-Fr Assay dependent
IHC-Wm Assay dependent
ELISA >1:20000
IHC Assay dependent
Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 and 0.01% (w/v) Sodium Azide and 10 mg/mL Bovine Serum Albumin (BSA)

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. Protect from light.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

The immunogen is a Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246aa) derived from the jellyfish Aequorea victoria.

Purification

Purified by antigen-affinity chromatography.
From polyclonal serum

Conjugation

Fluorescein isothiocyanate (FITC)
Absorption : 495 nm ; Emission : 528 nm. Ratio : 3.5 molecules FITC per Goat IgG molecule.

RRID

AB_371424

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Background

The jellyfish Aequorea victoria contains green fluorescent protein (GFP) that emits light in the bioluminescence reaction of the animal. GFP has been used widely as a reporter protein for gene expression in eukaryotic and prokaryotic organisms, and as a protein tag in cell culture and in multicellular organisms. As a fusion tag, GFP can be used to localize proteins, to study their movement or to research the dynamics of the subcellular compartments where these proteins are targeted. GFP technology has revealed considerable new insights into the physiological activities of living cells.

Research Area

DATA IMAGES

Anti-GFP antibody (FITC) used in IHC (Frozen sections) (IHC-Fr). GTX26662

GTX26662 IHC-Fr Image

Immunofluorescence Microscopy of GFP-GOAT-Antibody. Tissue: Sf-1:Cre mice crossed to the Z/EG reporter line. Mouse brain (coronal view, 20X magnification). Fixation: 4%PFA/PBS with o/n fixation, and subsequently transferred to a 30% sucrose solution. Antigen retrieval: frozen in OCT freezing medium (Sakura) and cryostat sectioned at 40 microns. Primary antibody: Goat anti-GFP (GTX26662) was used at 1:500 dilution in free floating imunnohistochemistry to detect GFP. Secondary antibody: Fluorchrome conjugated Anti-goat IgG secondary antibody was used for detection at 1:500 at 1:10,000 for 45 min at RT. Localization: Sf-1+ neurons and their processes of the ventromedial nucleus of the hypothalamus. Staining: eGFP as green fluorescent signal and sections were counterstained with DAPI.

Anti-GFP antibody (FITC) used in Western Blot (WB). GTX26662

GTX26662 WB Image

Western Blot of Goat anti-GFP Fluorescein Conjugated Antibody (GTX26662). Lane 1: GFP. Load: 50 ng per lane. Primary antibody: None. Secondary antibody: Fluorescein goat secondary antibody at 1:1,000 for 60 min at RT. Block for 30 min at RT. Predicted/Observed size: 28 kDa, 33 kDa for GFP. Other band(s): None.

REFERENCE

REVIEW

GFP antibody (FITC) Cat No. GTX26662

Rating ( Average 5 based on 5 users reviews)
Application
IHC (Whole mount)(IHC-Wm) ( Average 5 based on 1 users reviews)
Immunohistochemistry(IHC) ( Average 5 based on 4 users reviews)
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : IHC-Wm
Figure Note : The data was peer reviewed and published in the journal PLoS Genet in 2008.PMID: 18404215
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : IHC
Figure Note : The data was peer reviewed and published in the journal Nat Commun in 2016.PMID: 26960923
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : IHC
Figure Note : The data was peer reviewed and published in the journal Nat Commun in 2016.PMID: 26960923
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : IHC
Figure Note : The data was peer reviewed and published in the journal Nat Commun in 2016.PMID: 26960923
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : IHC
Figure Note : The data was peer reviewed and published in the journal Nat Commun in 2016.PMID: 26960923
Application Reference
SDS
Sodium Azide.pdf
Package List Price ($)
$ 339