GeneTex

  • Country / Location Selection

United States (US)

GFP antibody [GF28R]

Anti-GFP antibody [GF28R] used in Western Blot (WB). GTX82564

Cat. No. GTX82564

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 GF28R

Isotype

 IgG1

Application

 WB, ICC/IF, IP, ELISA

Reactivity

 Species independent
Package
100 μg ($399)
See all GFP products

APPLICATION

Application Note

Recommended Starting Dilutions:
WB (with ECL): Use at a dilution of 1:1000-3000
Immunostaining: Use at a dilution of 1:500 - 1:2000

For best results with other assays (e.g.: Dot, ELISA, IP, etc), please determine optimal working dilution by titration test

Product Note

Recognizes native and denatured forms of GFP and its variants such as: EGFP, YFP, EYFP, and CFP

PROPERTIES

Form

Liquid

Buffer

10mM PBS pH7.2

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

GFP from the jellyfish Aequorea Victoria N-terminal peptide-KLH conjugates

Purification

Protein A purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

eGFP , Green Fluorescent Protein , GFP , GFP Tag , Enhanced Green Fluorescent Protein

Background

A highly versatile antibody that gives a stronger signal than other anti-GFP antibodies available. On Western blot the antibody detects the GFP fraction from cell extracts expressing recombinant GFP fusion proteins and has also been shown to be useful on mouse sections fixed with formalin (Mesaeli et al. 1999). In Immunocytochemistry, the antibody gives a very good signal on recombinant YES-GFP chimeras expressed in COS cells (McCabe et al. 1999 and figure below). It is routinely used in Immunoprecipitation (IP) and IP-Western protocols and has been used successfully in HRP Immunohistochemistry at 1:200 on whole-mount mouse embryos.

Research Area

DATA IMAGES

Anti-GFP antibody [GF28R] used in Western Blot (WB). GTX82564

GTX82564 WB Image

1:1000 (1μg/mL) Ab dilution probed against HEK293 cells transfected with GFP-tagged protein vector: untransfected control (1), 1μg (2) and 10μg (3) of cell lysates used

REFERENCE

There are currently no references for GFP antibody [GF28R] (GTX82564). Be the first to share your publications with this product.
Submit a Reference

REVIEW

There are currently no reviews for GFP antibody [GF28R] (GTX82564). Be the first to share your experience with this product.
Submit a Review
SDS
PBS.pdf
Package List Price ($)
$ 399
Custom Conjugations