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GFP antibody [GT7312]

Anti-GFP antibody [GT7312] used in Immunoprecipitation (IP). GTX628894
Anti-GFP antibody [GT7312] used in Western Blot (WB). GTX628894
Anti-GFP antibody [GT7312] used in ELISA (ELISA). GTX628894

Cat No. GTX628894

Host

Mouse

Clonality

Monoclonal

Clone Name

GT7312

Isotype

IgG2a

Application

WB, IP, ELISA, Sandwich ELISA
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:5000-1:20000
IP 1:100-1:500
ELISA 1:1000-1:10000
Sandwich ELISA Assay dependent

Note :

Sandwich ELISA
Capture: GTX628894 , Detection: GTX113617

Not tested in other applications.

Calculated MW

29 kDa. ( Note )

Positive Control

293T whole cell extract expressing GFP-tagged protein

Product Note

This antibody reacts with GFP variants including GFP, EGFP, and YFP.

PROPERTIES

Form

Liquid

Buffer

PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.04 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

Full length GFP recombinant protein

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_2773724

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

DATA IMAGES

Anti-GFP antibody [GT7312] used in Immunoprecipitation (IP). GTX628894

GTX628894 IP Image

GFP tag antibody immunoprecipitates GFP-fused protein in IP experiments. IP Sample: 400 μg cell lysate/extract of GFP-fused gene transfected 293T cells A. 30 μg lysate/extract of transfected 293T cell B. Control with 2 μg of preimmune mouse IgG C. Immunoprecipitation of GFP-fused protein by 2 μg of GFP tag antibody (GTX628894) 12% SDS-PAGE The immunoprecipitated GFP-fused protein was detected by GFP tag antibody (GTX628894) diluted at 1:1000. EasyBlot anti-mouse IgG (GTX221667-01) was used as a secondary reagent.

Anti-GFP antibody [GT7312] used in Western Blot (WB). GTX628894

GTX628894 WB Image

GFP antibody detects GFP protein by western blot analysis.
A. 30 μg 293T whole cell extract
B. 30 μg 293T whole cell extract expressing GFP-tagged protein
C. 30 μg 293T whole cell extract expressing RFP-tagged protein
12% SDS-PAGE
GFP antibody (GTX628894) dilution: 1:10000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-GFP antibody [GT7312] used in ELISA (ELISA). GTX628894

GTX628894 ELISA Image

ELISA detection of GFP using GTX628894 for capture and GTX113617 for detection.
Coating Ab: mouse anti-GFP (GTX628894, 2μg/well )
Sample: GFP protein
Detected Ab: rabbit anti-GFP (GTX113617)
Conjugated Ab: goat anti-rabbit IgG-HRP (GTX213110-01, 1:20000)

REFERENCE

REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 329
$ 169