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GFP antibody [GT859] (HRP)

Anti-GFP antibody [GT859] (HRP) used in Western Blot (WB). GTX628528-01

Cat. No. GTX628528-01

Host

Mouse

Clonality

Monoclonal

Clone Name

GT859

Isotype

IgG2b

Application

WB
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:5000-1:20000
Not tested in other applications.

Calculated MW

29 kDa. ( Note )

Positive Control

293T expressing GFP-tagged protein

Product Note

This antibody reacts with GFP variants including GFP, EGFP, and YFP.

PROPERTIES

Form

Liquid

Buffer

0.05M Tris (pH7.4), 0.15M NaCl, 1%BSA

Preservative

0.005% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.36 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

Full length GFP recombinant protein

Purification

Affinity purified by Protein G.

Conjugation

Horseradish peroxidase(HRP)

RRID

AB_2888045

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

DATA IMAGES

Anti-GFP antibody [GT859] (HRP) used in Western Blot (WB). GTX628528-01

GTX628528-01 WB Image

GFP antibody (HRP) detects GFP protein by Western blot analysis.
A. 30 μg 293T whole cell extract
B. 30 μg 293T whole cell extract expressing GFP-tagged protein
C. 30 μg 293T whole cell extract expressing RFP-tagged protein
12 % SDS-PAGE
GFP antibody (HRP) (GTX628528-01) dilution: 1:10000

REFERENCE

REVIEW

GFP antibody [GT859] (HRP) Cat. No. GTX628528-01

Rating ( Average 5 based on 1 users reviews)
Application
Western Blot(WB) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 03-Oct-2022
Score :
Application Tested : WB
Figure Note : The data was peer reviewed and published in the 2019 in Nat Commun. PMID: 31477731
SDS
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169