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GFP antibody [GT859] (HRP)

Anti-GFP antibody [GT859] (HRP) used in Western Blot (WB). GTX628528-01

Cat. No. GTX628528-01

Host

Mouse

Clonality

Monoclonal

Clone Name

GT859

Isotype

IgG2b

Application

WB
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:5000-1:20000
Not tested in other applications.

Calculated MW

29 kDa. ( Note )

Positive Control

293T expressing GFP-tagged protein

Product Note

This antibody reacts with GFP variants including GFP, EGFP, and YFP.

PROPERTIES

Form

Liquid

Buffer

0.05M Tris, 0.15M NaCl, 1%BSA

Preservative

0.005% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.36 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

Full length GFP recombinant protein

Purification

Affinity purified by Protein G.

Conjugation

Horseradish peroxidase(HRP)

RRID

AB_2888045

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

DATA IMAGES

Anti-GFP antibody [GT859] (HRP) used in Western Blot (WB). GTX628528-01

GTX628528-01 WB Image

GFP antibody (HRP) detects GFP protein by Western blot analysis.
A. 30 μg 293T whole cell extract
B. 30 μg 293T whole cell extract expressing GFP-tagged protein
C. 30 μg 293T whole cell extract expressing RFP-tagged protein
12 % SDS-PAGE
GFP antibody (HRP) (GTX628528-01) dilution: 1:10000

REFERENCE

REVIEW

GFP antibody [GT859] (HRP) Cat. No. GTX628528-01

Rating ( Average 5 based on 1 users reviews)
Application
Western Blot(WB) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 03-Oct-2022
Score :
Application Tested : WB
Figure Note : The data was peer reviewed and published in the 2019 in Nat Commun. PMID: 31477731
Application Reference
Clone Reference
SDS
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169