GFP antibody [GT859] (HRP)

Name: GFP antibody [GT859] (HRP)
Brand: GeneTex
SKU: GTX628528-01
Price: 399.00 USD
Rating: 5 (1 reviews)

Anti-GFP antibody [GT859] (HRP) used in Western Blot (WB). GTX628528-01

Cat. No. GTX628528-01

Reference 1

Host	Mouse
Clonality	Monoclonal
Clone Name	GT859
Isotype	IgG2b
Application	WB
Package	100 μl ($399), 25 μl ($169)

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APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:5000-1:20000

Not tested in other applications.

Calculated MW

29 kDa. ( Note )

Positive Control

293T expressing GFP-tagged protein

Product Note

This antibody reacts with GFP variants including GFP, EGFP, and YFP.

PROPERTIES

Form

Liquid

Buffer

0.05M Tris (pH7.4), 0.15M NaCl, 1%BSA

Preservative

0.005% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.36 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

Full length GFP recombinant protein

Purification

Affinity purified by Protein G.

Conjugation

Horseradish peroxidase(HRP)

RRID

AB_2888045

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Research Area

DATA IMAGES

GTX628528-01 WB Image

GFP antibody (HRP) detects GFP protein by Western blot analysis.
A. 30 μg 293T whole cell extract
B. 30 μg 293T whole cell extract expressing GFP-tagged protein
C. 30 μg 293T whole cell extract expressing RFP-tagged protein
12 % SDS-PAGE
GFP antibody (HRP) (GTX628528-01) dilution: 1:10000

REFERENCE

Application Reference

Gorinski N et al. Nat Commun 2019; 10 (1) : 3924 Attenuated palmitoylation of serotonin receptor 5-HT1A affects receptor function and contributes to depression-like behaviors.

Application : WB

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