GeneTex
  • Country / Location Selection

United States (US)

GFP antibody [GT859]

Anti-GFP antibody [GT859] used in Immunoprecipitation (IP). GTX628528
Anti-GFP antibody [GT859] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX628528
Anti-GFP antibody [GT859] used in Western Blot (WB). GTX628528
Anti-GFP antibody [GT859] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX628528
Anti-GFP antibody [GT859] used in ELISA (ELISA). GTX628528
Anti-GFP antibody [GT859] used in Western Blot (WB). GTX628528

Cat No. GTX628528

Host

Mouse

Clonality

Monoclonal

Clone Name

GT859

Isotype

IgG2b

Application

WB, ICC/IF, IHC-P, IHC-Fr, IHC-Wm, IP, ELISA, Sandwich ELISA
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:2000
IHC-P Assay dependent
IHC-Fr Assay dependent
IHC-Wm Assay dependent
IP 1:100-1:500
ELISA 1:1000-1:10000
Sandwich ELISA Assay dependent

Note :

Sandwich ELISA
Capture: GTX628528 , Detection: GTX113617

Not tested in other applications.

Positive Control

293T whole cell extract expressing GFP-tagged protein

Specificity/Sensitivity

This antibody reacts with GFP variants including GFP, EGFP, and YFP.

PROPERTIES

Form

Liquid

Buffer

PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.25 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

Full length GFP recombinant protein

Purification

Affinity purified by Protein A.
From tissue culture supernatant

Conjugation

Unconjugated

RRID

AB_2888044

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

DATA IMAGES

Anti-GFP antibody [GT859] used in Immunoprecipitation (IP). GTX628528

GTX628528 IP Image

GFP tag antibody immunoprecipitates GFP-fused protein in IP experiments. IP Sample: 400 μg cell lysate/extract of GFP-fused gene transfected 293T cells A. 30 μg lysate/extract of transfected 293T cell B. Control with 2 μg of preimmune mouse IgG C. Immunoprecipitation of GFP-fused protein by 2 μg of GFP tag antibody (GTX628528) 12% SDS-PAGE The immunoprecipitated GFP-fused protein was detected by GFP tag antibody (GTX628528) diluted at 1:1000. EasyBlot anti-mouse IgG (GTX221667-01) was used as a secondary reagent.

Anti-GFP antibody [GT859] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX628528

GTX628528 ICC/IF Image

GFP antibody [GT859] detects GFP protein by immunofluorescent analysis.
Sample: GFP-tag tagged protein transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Red: GFP stained by GFP antibody [GT859] (GTX628528) diluted at 1:2000.
Blue: Hoechst 33342 staining.

Anti-GFP antibody [GT859] used in Western Blot (WB). GTX628528

GTX628528 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (5 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with GFP antibody [GT859] (GTX628528) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-GFP antibody [GT859] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX628528

GTX628528 ICC/IF Image

GFP-tag antibody detects GFP-recombinant protein by immunofluorescent analysis. Sample: GFP-tag transfected HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: GFP is expressed in the tranfected cell Red: .GFP-recombinant protein stained by GFP-tag antibody (GTX628528) diluted at 1:2000. Blue: Hoechst 33342 staining.

Anti-GFP antibody [GT859] used in ELISA (ELISA). GTX628528

GTX628528 ELISA Image

ELISA detection of GFP using GTX628528 for capture and GTX113617 for detection.
Coating Ab: mouse anti-GFP (GTX628528, 2μg/well )
Sample: GFP protein
Detected Ab: rabbit anti-GFP (GTX113617)
Conjugated Ab: goat anti-rabbit IgG-HRP (GTX213110-01, 1:20000)

Anti-GFP antibody [GT859] used in Western Blot (WB). GTX628528

GTX628528 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (5 μg) were separated by 12% SDS-PAGE, and the membranes were blotted with GFP antibody [GT859] (GTX628528) diluted at 1:5000 and competitor's antibody (sc-9996) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

REFERENCE

REVIEW

GFP antibody [GT859] Cat No. GTX628528

Rating ( Average 5 based on 3 users reviews)
Application
Western Blot(WB) ( Average 5 based on 3 users reviews)
Immunoprecipitation(IP) ( Average 5 based on 2 users reviews)
Date : Anonymous submitted on 23-Dec-2020
Score :
Application Tested : WB
Figure Note : The data was pear reviewed and published in the journal Sci Rep in 2015.PMID: 25740706
Date : Anonymous submitted on 23-Dec-2020
Score :
Application Tested : WB,IP
Figure Note : The data was pear reviewed and published in the journal Sci Rep in 2015.PMID: 25740706
Date : Anonymous submitted on 23-Dec-2020
Score :
Application Tested : WB,IP
Figure Note : The data was pear reviewed and published in the journal Sci Rep in 2015.PMID: 25740706
Application Reference
SDS
PBS.pdf
Package List Price ($)
$ 329
$ 169