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GFP antibody [GT859]

Comparison
Anti-GFP antibody [GT859] used in Immunoprecipitation (IP). GTX628528
Anti-GFP antibody [GT859] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX628528
Anti-GFP antibody [GT859] used in Western Blot (WB). GTX628528
Anti-GFP antibody [GT859] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX628528
Anti-GFP antibody [GT859] used in ELISA (ELISA). GTX628528
Anti-GFP antibody [GT859] used in Western Blot (WB). GTX628528

Cat No. GTX628528

Reference 21
3

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 GT859

Isotype

 IgG2b

Application

 WB, ICC/IF, IHC-P, IHC-Fr, IHC-Wm, IP, ELISA, Sandwich ELISA
Package
100 μl ($329),
25 μl ($169)
See all GFP products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:2000
IHC-P Assay dependent
IHC-Fr Assay dependent
IHC-Wm Assay dependent
IP 1:100-1:500
ELISA 1:1000-1:10000
Sandwich ELISA Assay dependent

Note :

Sandwich ELISA
Capture: GTX628528 , Detection: GTX113617
Not tested in other applications.

Positive Control

293T whole cell extract expressing GFP-tagged protein

Specificity/Sensitivity

This antibody reacts with GFP variants including GFP, EGFP, and YFP.

PROPERTIES

Form

Liquid

Buffer

PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.25 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

Full length GFP recombinant protein

Purification

Affinity purified by Protein A.
From tissue culture supernatant

Conjugation

Unconjugated

RRID

AB_2888044

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Research Area

DATA IMAGES

Anti-GFP antibody [GT859] used in Immunoprecipitation (IP). GTX628528

GTX628528 IP Image

GFP tag antibody immunoprecipitates GFP-fused protein in IP experiments. IP Sample: 400 μg cell lysate/extract of GFP-fused gene transfected 293T cells A. 30 μg lysate/extract of transfected 293T cell B. Control with 2 μg of preimmune mouse IgG C. Immunoprecipitation of GFP-fused protein by 2 μg of GFP tag antibody (GTX628528) 12% SDS-PAGE The immunoprecipitated GFP-fused protein was detected by GFP tag antibody (GTX628528) diluted at 1:1000. EasyBlot anti-mouse IgG (GTX221667-01) was used as a secondary reagent.

Anti-GFP antibody [GT859] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX628528

GTX628528 ICC/IF Image

GFP antibody [GT859] detects GFP protein by immunofluorescent analysis.
Sample: GFP-tag tagged protein transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Red: GFP stained by GFP antibody [GT859] (GTX628528) diluted at 1:2000.
Blue: Hoechst 33342 staining.

Anti-GFP antibody [GT859] used in Western Blot (WB). GTX628528

GTX628528 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (5 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with GFP antibody [GT859] (GTX628528) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-GFP antibody [GT859] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX628528

GTX628528 ICC/IF Image

GFP-tag antibody detects GFP-recombinant protein by immunofluorescent analysis. Sample: GFP-tag transfected HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: GFP is expressed in the tranfected cell Red: .GFP-recombinant protein stained by GFP-tag antibody (GTX628528) diluted at 1:2000. Blue: Hoechst 33342 staining.

Anti-GFP antibody [GT859] used in ELISA (ELISA). GTX628528

GTX628528 ELISA Image

ELISA detection of GFP using GTX628528 for capture and GTX113617 for detection.
Coating Ab: mouse anti-GFP (GTX628528, 2μg/well )
Sample: GFP protein
Detected Ab: rabbit anti-GFP (GTX113617)
Conjugated Ab: goat anti-rabbit IgG-HRP (GTX213110-01, 1:20000)

Anti-GFP antibody [GT859] used in Western Blot (WB). GTX628528

GTX628528 WB Image

Comparison

Non-transfected (–) and transfected (+) 293T whole cell extracts (5 μg) were separated by 12% SDS-PAGE, and the membranes were blotted with GFP antibody [GT859] (GTX628528) diluted at 1:5000 and competitor's antibody (sc-9996) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

REFERENCE

Application Reference

Muniesh MS et al. Mol Biol Cell 2020; 31 (26) : 2932-2947 PTP-3 phosphatase promotes intramolecular folding of SYD-2 to inactivate kinesin-3 UNC-104 in neurons.
Application : WB, IP
Yin P et al. Acta Neuropathol 2019; 137 (6) : 919-937 Caspase-4 mediates cytoplasmic accumulation of TDP-43 in the primate brains.
Muniesh Muthaiyan Shanmugam et al. bioRxiv 2019; (Epub) PTP-3(LAR PTPR) promotes intramolecular folding of SYD-2(liprin-α) to inactivate UNC-104(KIF1A) in neurons.
Application : IP
Sai K et al. J Cell Biol 2019; (Epub) Necroptosis mediators RIPK3 and MLKL suppress intracellular Listeria replication independently of host cell killing.
Application : WB
Burger K et al. Nucleic Acids Res 2019; (Epub) Tyrosine kinase c-Abl couples RNA polymerase II transcription to DNA double-strand breaks.
Show More +
Submit a Reference

REVIEW

GFP antibody [GT859] Cat No. GTX628528

Rating
( Average 5 based on 3 users reviews)
Application
Western Blot(WB)
( Average 5 based on 3 users reviews)
Immunoprecipitation(IP)
( Average 5 based on 2 users reviews)
Anti-GFP antibody [GT859] used in Western Blot (WB). GTX628528
Date : Anonymous submitted on 23-Dec-2020
Score :
Application Tested : WB
Figure Note : The data was pear reviewed and published in the journal Sci Rep in 2015.PMID: 25740706
Anti-GFP antibody [GT859] used in Western Blot and Immunoprecipitation (WB IP). GTX628528
Date : Anonymous submitted on 23-Dec-2020
Score :
Application Tested : WB,IP
Figure Note : The data was pear reviewed and published in the journal Sci Rep in 2015.PMID: 25740706
Anti-GFP antibody [GT859] used in Western Blot and Immunoprecipitation (WB IP). GTX628528
Date : Anonymous submitted on 23-Dec-2020
Score :
Application Tested : WB,IP
Figure Note : The data was pear reviewed and published in the journal Sci Rep in 2015.PMID: 25740706
Submit a Review
Application Reference
Muniesh MS et al. Mol Biol Cell 2020; 31 (26):2932-2947 PTP-3 phosphatase promotes intramolecular folding of SYD-2 to inactivate kinesin-3 UNC-104 in neurons.
Application : WB, IP
Yin P et al. Acta Neuropathol 2019; 137 (6):919-937 Caspase-4 mediates cytoplasmic accumulation of TDP-43 in the primate brains.
Muniesh Muthaiyan Shanmugam et al. bioRxiv 2019; (Epub) PTP-3(LAR PTPR) promotes intramolecular folding of SYD-2(liprin-α) to inactivate UNC-104(KIF1A) in neurons.
Application : IP
Sai K et al. J Cell Biol 2019; (Epub) Necroptosis mediators RIPK3 and MLKL suppress intracellular Listeria replication independently of host cell killing.
Application : WB
Burger K et al. Nucleic Acids Res 2019; (Epub) Tyrosine kinase c-Abl couples RNA polymerase II transcription to DNA double-strand breaks.
Shen T et al. J Hematol Oncol 2018; 11 (1):95 Ube2v1-mediated ubiquitination and degradation of Sirt1 promotes metastasis of colorectal cancer by epigenetically suppressing autophagy.
Application : IP
Hung YH et al. Oncol Rep 2017; 37 (2):969-978 Argininosuccinate lyase interacts with cyclin A2 in cytoplasm and modulates growth of liver tumor cells.
Burger K et al. J Cell Biol 2017; 216 (8):2373-2389 Nuclear phosphorylated Dicer processes double-stranded RNA in response to DNA damage.
Application : IP
Lin HY et al. Algal Research 2017; 23 (2017):58-65 Alkaline phosphatase promoter as an efficient driving element for exogenic recombinant in the marine diatom Phaeodactylum tricornutum.
Application : WB
Afolayan AJ et al. J Biol Chem 2016; (Epub) Domain Mapping of the Heat Shock Protein 70 reveals that Glutamic acid 446 and Arginine 447 are Critical for Regulating Superoxide Dismutase-2 Function.
Application : WB
Mickum ML et al. Glycobiology 2016; 26 (3):270-85 Schistosoma mansoni α1,3-fucosyltransferase-F generates the Lewis X antigen.
Application : WB
Ren S et al. Am J Pathol 2016; (Epub) Inactivation of Tsc2 in Mesoderm-Derived Cells Causes Polycystic Kidney Lesions and Impairs Lung Alveolarization.
Application : IHC-P
Huang TY et al. J Neurosci 2016; 36 (30):7996-8011 SNX27 and SORLA Interact to Reduce Amyloidogenic Subcellular Distribution and Processing of Amyloid Precursor Protein.
Application : WB
Mankouri J et al. J Virol 2016; (Epub) Release of infectious hepatitis C virus from Huh7 cells occurs via a trans-Golgi network to endosome pathway independent of VLDL.
Application : WB
Huang CH et al. Biochim Biophys Acta 2016; 1863 (9):2212-2220 Negative pressure induces p120-catenin-dependent adherens junction disassembly in keratinocytes during wound healing.
Application : ICC/IF
Wang G et al. Proc Natl Acad Sci U S A 2016; 113 (12):3359-64 Ablation of huntingtin in adult neurons is nondeleterious but its depletion in young mice causes acute pancreatitis.
Application : WB
Liu Y et al. Sci Rep 2015; 5:8796 FOXK2 transcription factor suppresses ERα-positive breast cancer cell growth through down-regulating the stability of ERα via mechanism involving BRCA1/BARD1.
Application : WB, IP
Wu JC et al. Sci Rep 2015; 5:18476 Focal adhesion kinase-dependent focal adhesion recruitment of SH2 domains directs SRC into focal adhesions to regulate cell adhesion and migration.
Application : WB
Lin PC et al. Mol Plant Microbe Interact 2015; 28 (6):711-26 Application of an Integrated Omics Approach for Identifying Host Proteins That Interact With Odontoglossum ringspot virus Capsid Protein.
Application : WB
Yan S et al. Hum Mol Genet 2014; TDP-43 causes differential pathology in neuronal versus glial cells in the mouse brain.
Application : IHC-Fr
Shiau CE et al. Cell Rep 2013; 5 (5):1342-52 An anti-inflammatory NOD-like receptor is required for microglia development.
Application : IHC-Wm
SDS
PBS.pdf
Package List Price ($)
$ 329
$ 169