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GFP antibody [GT859]

Anti-GFP antibody [GT859] used in Immunoprecipitation (IP). GTX628528
Anti-GFP antibody [GT859] used in Western Blot (WB). GTX628528
Anti-GFP antibody [GT859] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX628528
Anti-GFP antibody [GT859] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX628528
Anti-GFP antibody [GT859] used in ELISA (ELISA). GTX628528
Anti-GFP antibody [GT859] used in Western Blot (WB). GTX628528
Anti-GFP antibody [GT859] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX628528
Anti-GFP antibody [GT859] used in Western Blot and Immunoprecipitation (WB IP). GTX628528
Anti-GFP antibody [GT859] used in Western Blot (WB). GTX628528
Anti-GFP antibody [GT859] used in Western Blot (WB). GTX628528
Anti-GFP antibody [GT859] used in Western Blot and Immunoprecipitation (WB IP). GTX628528
Anti-GFP antibody [GT859] used in Western Blot and Immunoprecipitation (WB IP). GTX628528
Anti-GFP antibody [GT859] used in Western Blot (WB). GTX628528
Anti-GFP antibody [GT859] used in Western Blot (WB). GTX628528
Anti-GFP antibody [GT859] used in Immunoprecipitation (IP). GTX628528
Anti-GFP antibody [GT859] used in Western Blot (WB). GTX628528

Cat. No. GTX628528

Host

Mouse

Clonality

Monoclonal

Clone Name

GT859

Isotype

IgG2b

Application

WB, ICC/IF, IHC-P, IHC-Fr, IHC-Wm, IP, ELISA, Sandwich ELISA

Reactivity

Species independent
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:2000
IHC-P Assay dependent
IHC-Fr Assay dependent
IHC-Wm Assay dependent
IP 1:100-1:500
ELISA 1:1000-1:10000
Sandwich ELISA Assay dependent

Note :

Sandwich ELISA
Capture: GTX628528 , Detection: GTX113617

Not tested in other applications.

Positive Control

GFP-transfected 293T

Product Note

This antibody reacts with GFP variants including GFP, EGFP, and YFP.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.25 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

Full length GFP recombinant protein

Purification

Affinity purified by Protein A.
From tissue culture supernatant

Conjugation

Unconjugated

RRID

AB_2888044

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

DATA IMAGES

Anti-GFP antibody [GT859] used in Immunoprecipitation (IP). GTX628528

GTX628528 IP Image

GFP tag antibody immunoprecipitates GFP-fused protein in IP experiments. IP Sample: 400 μg cell lysate/extract of GFP-fused gene transfected 293T cells A. 30 μg lysate/extract of transfected 293T cell B. Control with 2 μg of preimmune mouse IgG C. Immunoprecipitation of GFP-fused protein by 2 μg of GFP tag antibody (GTX628528) 12% SDS-PAGE The immunoprecipitated GFP-fused protein was detected by GFP tag antibody (GTX628528) diluted at 1:1000. EasyBlot anti-mouse IgG (GTX221667-01) was used as a secondary reagent.

Anti-GFP antibody [GT859] used in Western Blot (WB). GTX628528

GTX628528 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (5 μg) were separated by 12% SDS-PAGE, and the membranes were blotted with GFP antibody [GT859] (GTX628528) diluted at 1:5000 and competitor's antibody (sc-9996) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-GFP antibody [GT859] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX628528

GTX628528 ICC/IF Image

GFP-tag antibody detects GFP-recombinant protein by immunofluorescent analysis. Sample: GFP-tag transfected HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: GFP is expressed in the tranfected cell Red: .GFP-recombinant protein stained by GFP-tag antibody (GTX628528) diluted at 1:2000. Blue: Hoechst 33342 staining.

Anti-GFP antibody [GT859] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX628528

GTX628528 ICC/IF Image

GFP antibody [GT859] detects GFP protein by immunofluorescent analysis.
Sample: GFP-tag tagged protein transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Red: GFP stained by GFP antibody [GT859] (GTX628528) diluted at 1:2000.
Blue: Hoechst 33342 staining.

Anti-GFP antibody [GT859] used in ELISA (ELISA). GTX628528

GTX628528 ELISA Image

ELISA detection of GFP using GTX628528 for capture and GTX113617 for detection.
Coating Ab: mouse anti-GFP (GTX628528, 2μg/well )
Sample: GFP protein
Detected Ab: rabbit anti-GFP (GTX113617)
Conjugated Ab: goat anti-rabbit IgG-HRP (GTX213110-01, 1:20000)

Anti-GFP antibody [GT859] used in Western Blot (WB). GTX628528

GTX628528 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (5 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with GFP antibody [GT859] (GTX628528) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-GFP antibody [GT859] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX628528

GTX628528 ICC/IF Image

The data was published in the journal Nucleic Acids Res in 2019.PMID: 30668775

Anti-GFP antibody [GT859] used in Western Blot and Immunoprecipitation (WB IP). GTX628528

GTX628528 WB IP Image

The data was published in the journal J Hematol Oncol in 2018.PMID: 30016968

Anti-GFP antibody [GT859] used in Western Blot (WB). GTX628528

GTX628528 WB Image

The data was published in the journal Sci Rep in 2015.PMID: 26681405

Anti-GFP antibody [GT859] used in Western Blot (WB). GTX628528

GTX628528 WB Image

The data was published in the journal Acta Neuropathol in 2019.PMID: 30810811

Anti-GFP antibody [GT859] used in Western Blot and Immunoprecipitation (WB IP). GTX628528

GTX628528 WB IP Image

The data was published in the journal Sci Rep in 2015. PMID: 25740706

Anti-GFP antibody [GT859] used in Western Blot and Immunoprecipitation (WB IP). GTX628528

GTX628528 WB IP Image

The data was published in the journal Sci Rep in 2015. PMID: 25740706

Anti-GFP antibody [GT859] used in Western Blot (WB). GTX628528

GTX628528 WB Image

The data was published in the journal Sci Rep in 2015. PMID: 25740706

Anti-GFP antibody [GT859] used in Western Blot (WB). GTX628528

GTX628528 WB Image

The data was published in the journal Sci Rep in 2015.PMID: 26681405

Anti-GFP antibody [GT859] used in Immunoprecipitation (IP). GTX628528

GTX628528 IP Image

The data was published in the journal J Cell Biol in 2017.PMID: 28642363

Anti-GFP antibody [GT859] used in Western Blot (WB). GTX628528

GTX628528 WB Image

The data was published in the journal Sci Rep in 2015.PMID: 26681405

REFERENCE

REVIEW

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Application Reference
Clone Reference
SDS
PBS.pdf
Package List Price ($)
$ 399
$ 169