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GFP antibody [GT859]

Comparison
Anti-GFP antibody [GT859] used in Immunoprecipitation (IP). GTX628528
Anti-GFP antibody [GT859] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX628528
Anti-GFP antibody [GT859] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX628528
Anti-GFP antibody [GT859] used in ELISA (ELISA). GTX628528
Anti-GFP antibody [GT859] used in Western Blot (WB). GTX628528
Anti-GFP antibody [GT859] used in Western Blot (WB). GTX628528

Cat No. GTX628528

Reference 18
Host Mouse
Clonality Monoclonal
Clone Name GT859
Isotype IgG2b
Application WB, ICC/IF, IHC-P, IHC-Fr, IHC-Wm, IP, ELISA, sELISA
Package
100 μl ($319),
25 μl ($169)
See all GFP products
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:2000
IHC-P Assay dependent
IHC-Fr Assay dependent
IHC-Wm Assay dependent
IP 1:100-1:500
ELISA 1:1000-1:10000
sELISA Assay dependent

Note :

sELISA
Capture: GTX628528 , Detection: GTX113617
Not tested in other applications.

Positive Control

293T whole cell extract expressing GFP-tagged protein

Specificity/Sensitivity

This antibody reacts with GFP variants including GFP, EGFP, and YFP.
PROPERTIES

Form

Liquid

Buffer

PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.25 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

Full length GFP recombinant protein

Purification

Affinity purified by Protein A.
From tissue culture supernatant

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Research Area

DATA IMAGES
Anti-GFP antibody [GT859] used in Immunoprecipitation (IP). GTX628528

GTX628528 IP Image

GFP tag antibody immunoprecipitates GFP-fused protein in IP experiments. IP Sample: 400 µg cell lysate/extract of GFP-fused gene transfected 293T cells A. 30 μg lysate/extract of transfected 293T cell B. Control with 2 μg of preimmune mouse IgG C. Immunoprecipitation of GFP-fused protein by 2 μg of GFP tag antibody (GTX628528) 12% SDS-PAGE The immunoprecipitated GFP-fused protein was detected by GFP tag antibody (GTX628528) diluted at 1:1000. EasyBlot anti-mouse IgG (GTX221667-01) was used as a secondary reagent.

Anti-GFP antibody [GT859] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX628528

GTX628528 ICC/IF Image

GFP antibody [GT859] detects GFP protein by immunofluorescent analysis.
Sample: GFP-tag tagged protein transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Red: GFP stained by GFP antibody [GT859] (GTX628528) diluted at 1:2000.
Blue: Hoechst 33342 staining.

Anti-GFP antibody [GT859] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX628528

GTX628528 ICC/IF Image

GFP-tag antibody detects GFP-recombinant protein by immunofluorescent analysis. Sample: GFP-tag transfected HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: GFP is expressed in the tranfected cell Red: .GFP-recombinant protein stained by GFP-tag antibody (GTX628528) diluted at 1:2000. Blue: Hoechst 33342 staining.

Anti-GFP antibody [GT859] used in ELISA (ELISA). GTX628528

GTX628528 ELISA Image

ELISA detection of GFP using GTX628528 for capture and GTX113617 for detection.
Coating Ab: mouse anti-GFP (GTX628528, 2µg/well )
Sample: GFP protein
Detected Ab: rabbit anti-GFP (GTX113617)
Conjugated Ab: goat anti-rabbit IgG-HRP (GTX213110-01, 1:20000)

Anti-GFP antibody [GT859] used in Western Blot (WB). GTX628528

GTX628528 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (5 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with GFP antibody [GT859] (GTX628528) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-GFP antibody [GT859] used in Western Blot (WB). GTX628528

GTX628528 WB Image

Comparison

Non-transfected (–) and transfected (+) 293T whole cell extracts (5 μg) were separated by 12% SDS-PAGE, and the membranes were blotted with GFP antibody [GT859] (GTX628528) diluted at 1:5000 and competitor's antibody (sc-9996) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

REFERENCE

Application Reference

Muniesh Muthaiyan Shanmugam et al. bioRxiv 2019; (Epub) PTP-3(LAR PTPR) promotes intramolecular folding of SYD-2(liprin-α) to inactivate UNC-104(KIF1A) in neurons.
Application : IP
Sai K et al. J Cell Biol 2019; (Epub) Necroptosis mediators RIPK3 and MLKL suppress intracellular Listeria replication independently of host cell killing.
Application : WB
Burger K et al. Nucleic Acids Res 2019; (Epub) Tyrosine kinase c-Abl couples RNA polymerase II transcription to DNA double-strand breaks.
Shen T et al. J Hematol Oncol 2018; 11 (1) : 95 Ube2v1-mediated ubiquitination and degradation of Sirt1 promotes metastasis of colorectal cancer by epigenetically suppressing autophagy.
Application : IP
Burger K et al. J Cell Biol 2017; 216 (8) : 2373-2389 Nuclear phosphorylated Dicer processes double-stranded RNA in response to DNA damage.
Application : IP
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REVIEW
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Application Reference
Muniesh Muthaiyan Shanmugam et al. bioRxiv 2019; (Epub) PTP-3(LAR PTPR) promotes intramolecular folding of SYD-2(liprin-α) to inactivate UNC-104(KIF1A) in neurons.
Application : IP
Sai K et al. J Cell Biol 2019; (Epub) Necroptosis mediators RIPK3 and MLKL suppress intracellular Listeria replication independently of host cell killing.
Application : WB
Burger K et al. Nucleic Acids Res 2019; (Epub) Tyrosine kinase c-Abl couples RNA polymerase II transcription to DNA double-strand breaks.
Shen T et al. J Hematol Oncol 2018; 11 (1):95 Ube2v1-mediated ubiquitination and degradation of Sirt1 promotes metastasis of colorectal cancer by epigenetically suppressing autophagy.
Application : IP
Burger K et al. J Cell Biol 2017; 216 (8):2373-2389 Nuclear phosphorylated Dicer processes double-stranded RNA in response to DNA damage.
Application : IP
Lin HY et al. Algal Research 2017; 23 (2017):58-65 Alkaline phosphatase promoter as an efficient driving element for exogenic recombinant in the marine diatom Phaeodactylum tricornutum.
Application : WB
Afolayan AJ et al. J Biol Chem 2016; (Epub) Domain Mapping of the Heat Shock Protein 70 reveals that Glutamic acid 446 and Arginine 447 are Critical for Regulating Superoxide Dismutase-2 Function.
Application : WB
Mickum ML et al. Glycobiology 2016; 26 (3):270-85 Schistosoma mansoni α1,3-fucosyltransferase-F generates the Lewis X antigen.
Application : WB
Ren S et al. Am J Pathol 2016; (Epub) Inactivation of Tsc2 in Mesoderm-Derived Cells Causes Polycystic Kidney Lesions and Impairs Lung Alveolarization.
Application : IHC-P
Huang TY et al. J Neurosci 2016; 36 (30):7996-8011 SNX27 and SORLA Interact to Reduce Amyloidogenic Subcellular Distribution and Processing of Amyloid Precursor Protein.
Application : WB
Mankouri J et al. J Virol 2016; (Epub) Release of infectious hepatitis C virus from Huh7 cells occurs via a trans-Golgi network to endosome pathway independent of VLDL.
Application : WB
Huang CH et al. Biochim Biophys Acta 2016; 1863 (9):2212-2220 Negative pressure induces p120-catenin-dependent adherens junction disassembly in keratinocytes during wound healing.
Application : ICC/IF
Wang G et al. Proc Natl Acad Sci U S A 2016; 113 (12):3359-64 Ablation of huntingtin in adult neurons is nondeleterious but its depletion in young mice causes acute pancreatitis.
Application : WB
Liu Y et al. Sci Rep 2015; 5:8796 FOXK2 transcription factor suppresses ERα-positive breast cancer cell growth through down-regulating the stability of ERα via mechanism involving BRCA1/BARD1.
Application : WB, IP
Wu JC et al. Sci Rep 2015; 5:18476 Focal adhesion kinase-dependent focal adhesion recruitment of SH2 domains directs SRC into focal adhesions to regulate cell adhesion and migration.
Application : WB
Lin PC et al. Mol Plant Microbe Interact 2015; 28 (6):711-26 Application of an Integrated Omics Approach for Identifying Host Proteins That Interact With Odontoglossum ringspot virus Capsid Protein.
Application : WB
Yan S et al. Hum Mol Genet 2014; TDP-43 causes differential pathology in neuronal versus glial cells in the mouse brain.
Application : IHC-Fr
Shiau CE et al. Cell Rep 2013; 5 (5):1342-52 An anti-inflammatory NOD-like receptor is required for microglia development.
Application : IHC-Wm
SDS
PBS.pdf
Package List Price ($)
$ 319
$ 169