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GFP antibody [GT859]

Anti-GFP antibody [GT859] used in Immunoprecipitation (IP). GTX628528
Anti-GFP antibody [GT859] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX628528
Anti-GFP antibody [GT859] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX628528
Anti-GFP antibody [GT859] used in ELISA (ELISA). GTX628528
Anti-GFP antibody [GT859] used in Western Blot (WB). GTX628528
Anti-GFP antibody [GT859] used in Western Blot (WB). GTX628528

Cat No. GTX628528

Host Mouse
Clonality Monoclonal
Clone Name GT859
Isotype IgG2b
Application WB, ICC/IF, IHC-P, IHC-Fr, IHC-Wm, IP, ELISA, sELISA
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:2000
IHC-P Assay dependent
IHC-Fr Assay dependent
IHC-Wm Assay dependent
IP 1:100-1:500
ELISA 1:1000-1:10000
sELISA Assay dependent

Note :

sELISA
Capture: GTX628528 , Detection: GTX113617

Not tested in other applications.

Positive Control

293T whole cell extract expressing GFP-tagged protein

Specificity/Sensitivity

This antibody reacts with GFP variants including GFP, EGFP, and YFP.
PROPERTIES

Form

Liquid

Buffer

PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.25 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

Full length GFP recombinant protein

Purification

Affinity purified by Protein A.
From tissue culture supernatant

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Research Area

DATA IMAGES
Anti-GFP antibody [GT859] used in Immunoprecipitation (IP). GTX628528

GTX628528 IP Image

GFP tag antibody immunoprecipitates GFP-fused protein in IP experiments. IP Sample: 400 µg cell lysate/extract of GFP-fused gene transfected 293T cells A. 30 μg lysate/extract of transfected 293T cell B. Control with 2 μg of preimmune mouse IgG C. Immunoprecipitation of GFP-fused protein by 2 μg of GFP tag antibody (GTX628528) 12% SDS-PAGE The immunoprecipitated GFP-fused protein was detected by GFP tag antibody (GTX628528) diluted at 1:1000. EasyBlot anti-mouse IgG (GTX221667-01) was used as a secondary reagent.

Anti-GFP antibody [GT859] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX628528

GTX628528 ICC/IF Image

GFP antibody [GT859] detects GFP protein by immunofluorescent analysis.
Sample: GFP-tag tagged protein transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Red: GFP stained by GFP antibody [GT859] (GTX628528) diluted at 1:2000.
Blue: Hoechst 33342 staining.

Anti-GFP antibody [GT859] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX628528

GTX628528 ICC/IF Image

GFP-tag antibody detects GFP-recombinant protein by immunofluorescent analysis. Sample: GFP-tag transfected HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: GFP is expressed in the tranfected cell Red: .GFP-recombinant protein stained by GFP-tag antibody (GTX628528) diluted at 1:2000. Blue: Hoechst 33342 staining.

Anti-GFP antibody [GT859] used in ELISA (ELISA). GTX628528

GTX628528 ELISA Image

ELISA detection of GFP using GTX628528 for capture and GTX113617 for detection.
Coating Ab: mouse anti-GFP (GTX628528, 2µg/well )
Sample: GFP protein
Detected Ab: rabbit anti-GFP (GTX113617)
Conjugated Ab: goat anti-rabbit IgG-HRP (GTX213110-01, 1:20000)

Anti-GFP antibody [GT859] used in Western Blot (WB). GTX628528

GTX628528 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (5 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with GFP antibody [GT859] (GTX628528) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-GFP antibody [GT859] used in Western Blot (WB). GTX628528

GTX628528 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (5 μg) were separated by 12% SDS-PAGE, and the membranes were blotted with GFP antibody [GT859] (GTX628528) diluted at 1:5000 and competitor's antibody (sc-9996) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

REFERENCE
REVIEW
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Application Reference
SDS
PBS.pdf
Package List Price ($)
$ 319
$ 169