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GFP antibody [HL1845]

Recombinant antibody
Anti-GFP antibody [HL1845] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637570
Anti-GFP antibody [HL1845] used in Western Blot (WB). GTX637570
Anti-GFP antibody [HL1845] used in Western Blot (WB). GTX637570
Anti-GFP antibody [HL1845] used in IHC-P (cell pellet) (IHC-P (cell pellet)). GTX637570

Cat. No. GTX637570

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL1845

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P (cell pellet)

Reactivity

 Species independent
Package
100 μl ($399),
25 μl ($169)
See all GFP products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
ICC/IF Assay dependent
IHC-P (cell pellet) Assay dependent
Not tested in other applications.

Product Note

This antibody reacts with GFP variants including GFP, EGFP, and YFP.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

Full length GFP recombinant protein

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Research Area

DATA IMAGES

Anti-GFP antibody [HL1845] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637570

GTX637570 ICC/IF Image

GFP-tag antibody detects GFP tagged protein by immunofluorescent analysis.
Sample: GFP tagged protein transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: GFP tagged protein stained by GFP-tag antibody (GTX637570) diluted at 1500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-GFP antibody [HL1845] used in Western Blot (WB). GTX637570

GTX637570 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with GFP antibody [HL1845] (GTX637570) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-GFP antibody [HL1845] used in Western Blot (WB). GTX637570

GTX637570 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with GFP antibody [HL1845] (GTX637570) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-GFP antibody [HL1845] used in IHC-P (cell pellet) (IHC-P (cell pellet)). GTX637570

GTX637570 IHC-P (cell pellet) Image

GFP antibody [HL1845] detects GFP protein by immunohistochemical analysis.
Sample:Paraffin-embedded mock and GFP-tagged protein transfected 293T.
Red: GFP-tagged protein stained by GFP antibody [HL1845] (GTX637570) diluted at 1:1000.
Green: GFP-tagged protein is expressed in the transfected cells.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations