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GFP antibody (HRP)

Anti-GFP antibody (HRP) used in Western Blot (WB). GTX113618-01

Cat. No. GTX113618-01

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
Not tested in other applications.

Positive Control

HeLa expressing GFP-tagged protein

PROPERTIES

Form

Liquid

Buffer

TBS pH7.4, 10mg/ml BSA

Preservative

0.005% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.735 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

Recombinant protein conjugated synthetic peptide encompassing a sequence within the center region of GFP. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Horseradish peroxidase(HRP)

RRID

AB_11174406

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Green Fluorescent Protein , GFP

Background

The jellyfish Aequorea victoria contains green fluorescent protein (GFP) that emits light in the bioluminescence reaction of the animal. GFP has been used widely as a reporter protein for gene expression in eukaryotic and prokaryotic organisms, and as a protein tag in cell culture and in multicellular organisms. As a fusion tag, GFP can be used to localize proteins, to study their movement or to research the dynamics of the subcellular compartments where these proteins are targeted. GFP technology has revealed considerable new insights into the physiological activities of living cells.

Research Area

DATA IMAGES

Anti-GFP antibody (HRP) used in Western Blot (WB). GTX113618-01

GTX113618-01 WB Image

GFP antibody (HRP) detects GFP protein by Western blot analysis.
A. 4 μg HeLa whole cell extract
B. 4 μg HeLa whole cell extract expressing GFP-tagged protein
12 % SDS-PAGE
GFP antibody (HRP) (GTX113618-01) dilution: 1:4000

REFERENCE

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REVIEW

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SDS
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169