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GFP antibody

Anti-GFP antibody used in Western Blot (WB). GTX113618

Cat No. GTX113618

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:5000-1:20000
Not tested in other applications.

Calculated MW

29 kDa. ( Note )

Positive Control

293T whole cell extract expressing GFP-tagged protein

Specificity/Sensitivity

This antibody reacts with GFP variants including GFP, EGFP, and YFP.

PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

Recombinant protein conjugated synthetic peptide encompassing a sequence within the center region of GFP. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Green Fluorescent Protein , GFP

Research Area

DATA IMAGES

Anti-GFP antibody used in Western Blot (WB). GTX113618

GTX113618 WB Image

GFP antibody detects GFP protein by Western blot analysis.
A. 30 μg 293T whole cell extract
B. 30 μg 293T whole cell extract expressing GFP-tagged protein
C. 30 μg 293T whole cell extract expressing RFP-tagged protein
12 % SDS-PAGE
GFP antibody (GTX113618) dilution: 1:10000

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169