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GFP antibody

Anti-GFP antibody used in Western Blot (WB). GTX124117

Cat. No. GTX124117

Reference 2
1

Host

 Chicken

Clonality

 Polyclonal

Isotype

 IgY

Application

 WB

Reactivity

 Species independent
Package
100 μl ($399),
25 μl ($169)
See all GFP products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:40000
Not tested in other applications.

Positive Control

HeLa expressing GFP-tagged protein

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

Full length GFP recombinant protein

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11163673

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Background

The jellyfish Aequorea victoria contains green fluorescent protein (GFP) that emits light in the bioluminescence reaction of the animal. GFP has been used widely as a reporter protein for gene expression in eukaryotic and prokaryotic organisms, and as a protein tag in cell culture and in multicellular organisms. As a fusion tag, GFP can be used to localize proteins, to study their movement or to research the dynamics of the subcellular compartments where these proteins are targeted. GFP technology has revealed considerable new insights into the physiological activities of living cells.

Research Area

DATA IMAGES

Anti-GFP antibody used in Western Blot (WB). GTX124117

GTX124117 WB Image

GFP antibody detects GFP protein by Western blot analysis.
A. 4 μg HeLa whole cell extract
B. 4 μg HeLa whole cell extract expressing GFP-tagged protein
12 % SDS-PAGE
GFP antibody (GTX124117) dilution: 1:40000

REFERENCE

Application Reference

Yamamoto W et al. J Cell Sci 2017; (Epub) Distinct roles for plasma membrane PtdIns(4)P and PtdIns(4,5)P2 during yeast receptor-mediated endocytosis.
Application : WB
Toshima JY et al. Elife 2016; 5 Yeast Eps15-like endocytic protein Pan1p regulates the interaction between endocytic vesicles, endosomes and the actin cytoskeleton.
Application : WB
Submit a Reference

REVIEW

GFP antibody Cat. No. GTX124117

Rating
( Average 5 based on 1 users reviews)
Application
Western Blot(WB)
( Average 5 based on 1 users reviews)
Anti-GFP antibody used in Western Blot (WB). GTX124117
Date : Anonymous submitted on 18-Oct-2018
Score :
Application Tested : WB
Sample : BY4741 (budding yeast) expressing GFP(S65T)-tagged Vps21p (small GTPase Rab5 homologue)
Amount used : 5 μg
Blocking : 1% no-fat milk in PBS-0.05% Tween-20, 25°C, 0.5Hr
Primary Antibody : 1:10000, 4°C, 5Hr
Submit a Review
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169