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GFP antibody

Anti-GFP antibody used in Immunohistochemistry (IHC). GTX13970

Cat No. GTX13970

Host

Chicken

Clonality

Polyclonal

Isotype

IgY

Application

WB, ICC/IF, IHC-Fr, IHC-Wm, ELISA, IHC
Package
100 μl ($339)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:5000-1:10000
ICC/IF Assay dependent
IHC-Fr Assay dependent
IHC-Wm Assay dependent
ELISA Assay dependent
IHC 1:2000-1:5000
Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

Sodium phosphate (10 mM, pH 7.2) buffered isotonic saline (0.9%), 50% glycerol, containing 0.01% thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.

Concentration

10 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

Chickens were immunized with purified recombinant green fluorescent protein (GFP) emulsified in Freund’s adjuvant.

Purification

Purified by antigen-affinity chromatography
Serile filtered.

Conjugation

Unconjugated

RRID

AB_371416

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Green Fluorescent Protein

Background

The jellyfish Aequorea victoria contains green fluorescent protein (GFP) that emits light in the bioluminescence reaction of the animal. GFP has been used widely as a reporter protein for gene expression in eukaryotic and prokaryotic organisms, and as a protein tag in cell culture and in multicellular organisms. As a fusion tag, GFP can be used to localize proteins, to study their movement or to research the dynamics of the subcellular compartments where these proteins are targeted. GFP technology has revealed considerable new insights into the physiological activities of living cells.

Research Area

DATA IMAGES

Anti-GFP antibody used in Immunohistochemistry (IHC). GTX13970

GTX13970 IHC Image

IHC analysis of paraformaldehyde-fixed (4%) neonatal mouse brain using GTX13970 GFP antibody.

REFERENCE

REVIEW

GFP antibody Cat No. GTX13970

Rating ( Average 5 based on 3 users reviews)
Application
IHC (Whole mount)(IHC-Wm) ( Average 5 based on 1 users reviews)
Immunohistochemistry(IHC) ( Average 5 based on 2 users reviews)
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : IHC-Wm
Figure Note : The data was peer reviewed and published in the journal Elife in 2017.PMID: 28832322
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : IHC
Figure Note : The data was peer reviewed and published in the journal Sci Rep in 2017.PMID: 28959031
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : IHC
Figure Note : The data was peer reviewed and published in the journal Elife in 2014.PMID: 25490154
Application Reference
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 339