GeneTex
  • Country / Location Selection

United States (US)

GFP antibody

Cat No. GTX20290

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, IHC-Fr, FACS, IP, ELISA, ChIP assay, Gel supershift assays, IHC, IM, IHC (Free Floating)
Package
50 μl ($359)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB Assay dependent
ICC/IF Assay dependent
IHC-P Assay dependent
IHC-Fr Assay dependent
FACS Assay dependent
IP Assay dependent
ELISA Assay dependent
ChIP assay Assay dependent
Gel supershift assays Assay dependent
IHC Assay dependent
IM Assay dependent
IHC (Free Floating) Assay dependent
Not tested in other applications.

Product Note

Reactive against all variants of Aequorea victoria GFP such as S65T-GFP, RS-GFP, YFP and EGFP.

PROPERTIES

Form

Liquid

Buffer

Serum, 0.01% thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Aequorea victoria

Immunogen

The GFP was isolated directly from the jellyfish Aequorea victoria.

Purification

Unpurified

Conjugation

Unconjugated

RRID

AB_371415

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Background

A highly versatile antibody that gives a stronger signal than other anti-GFP antibodies available. On Western blot the antibody detects the GFP fraction from cell extracts expressing recombinant GFP fusion proteins and has also been shown to be useful on mouse sections fixed with formalin (Mesaeli et al. 1999). In Immunocytochemistry, the antibody gives a very good signal on recombinant YES-GFP chimeras expressed in COS cells (McCabe et al. 1999 and figure below). It is routinely used in Immunoprecipitation (IP) and IP-Western protocols and has been used successfully in HRP Immunohistochemistry at 1:200 on whole-mount mouse embryos.

Research Area

REFERENCE

REVIEW

GFP antibody Cat No. GTX20290

Rating ( Average 5 based on 6 users reviews)
Application
Western Blot(WB) ( Average 5 based on 1 users reviews)
Immunocytochemistry/ Immunofluorescence(ICC/IF) ( Average 5 based on 5 users reviews)
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Figure Note : The data was peer reviewed and published in the journal PLoS One in 2013.PMID: 23737990
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : ICC/IF
Figure Note : The data was peer reviewed and published in the journal EMBO J in 2019.PMID: 31368584
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : ICC/IF
Figure Note : The data was peer reviewed and published in the journal EMBO J in 2019.PMID: 31368584
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : ICC/IF
Figure Note : The data was peer reviewed and published in the journal EMBO J in 2019.PMID: 31368584
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : ICC/IF
Figure Note : The data was peer reviewed and published in the journal EMBO J in 2019.PMID: 31368584
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : ICC/IF
Figure Note : The data was peer reviewed and published in the journal J Neurochem in 2018.PMID: 29178418
Application Reference
SDS
Thimerosal.pdf
Package List Price ($)
$ 359