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GFP antibody

Anti-GFP antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX20290
Anti-GFP antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX20290
Anti-GFP antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX20290
Anti-GFP antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX20290
Anti-GFP antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX20290
Anti-GFP antibody used in Western Blot (WB). GTX20290

Cat. No. GTX20290

Reference 20

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC-Fr, FACS, IP, ELISA, ChIP assay, Gel supershift assays, IHC, IM, IHC (Free Floating)

Reactivity

 Species independent
Package
50 μl ($399)
See all GFP products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
ICC/IF Assay dependent
IHC-P Assay dependent
IHC-Fr Assay dependent
FACS Assay dependent
IP Assay dependent
ELISA Assay dependent
ChIP assay Assay dependent
Gel supershift assays Assay dependent
IHC Assay dependent
IM Assay dependent
IHC (Free Floating) Assay dependent
Not tested in other applications.

Product Note

Reactive against all variants of Aequorea victoria GFP such as S65T-GFP, RS-GFP, YFP and EGFP.

PROPERTIES

Form

Liquid

Buffer

Serum

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Aequorea victoria

Immunogen

The GFP was isolated directly from the jellyfish Aequorea victoria.

Purification

Unpurified

Conjugation

Unconjugated

RRID

AB_371415

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Background

A highly versatile antibody that gives a stronger signal than other anti-GFP antibodies available. On Western blot the antibody detects the GFP fraction from cell extracts expressing recombinant GFP fusion proteins and has also been shown to be useful on mouse sections fixed with formalin (Mesaeli et al. 1999). In Immunocytochemistry, the antibody gives a very good signal on recombinant YES-GFP chimeras expressed in COS cells (McCabe et al. 1999 and figure below). It is routinely used in Immunoprecipitation (IP) and IP-Western protocols and has been used successfully in HRP Immunohistochemistry at 1:200 on whole-mount mouse embryos.

Research Area

DATA IMAGES

Anti-GFP antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX20290

GTX20290 ICC/IF Image

The data was published in the journal J Neurochem in 2018. PMID: 29178418

Anti-GFP antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX20290

GTX20290 ICC/IF Image

The data was published in the journal EMBO J in 2019. PMID: 31368584

Anti-GFP antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX20290

GTX20290 ICC/IF Image

The data was published in the journal EMBO J in 2019. PMID: 31368584

Anti-GFP antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX20290

GTX20290 ICC/IF Image

The data was published in the journal EMBO J in 2019. PMID: 31368584

Anti-GFP antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX20290

GTX20290 ICC/IF Image

The data was published in the journal EMBO J in 2019. PMID: 31368584

Anti-GFP antibody used in Western Blot (WB). GTX20290

GTX20290 WB Image

The data was published in the journal PLoS One in 2013. PMID: 23737990

REFERENCE

Application Reference

Thosar SA et al. Cell Rep 2024; 43 (1) : 113656 Oxidative guanine base damage plays a dual role in regulating productive ALT-associated homology-directed repair.
Laspata N et al. Nucleic Acids Res 2023; 51 (5) : 2215-2237 PARP1 associates with R-loops to promote their resolution and genome stability.
Application : WB
Boris Simonetti et al. PLoS Biol 2022; 20 (4) : e3001601 SNX27-Retromer directly binds ESCPE-1 to transfer cargo proteins during endosomal recycling.
Application : WB
Boris Simonetti et al. Proc Natl Acad Sci U S A 2022; 119 (25) : e2201980119 ESCPE-1 mediates retrograde endosomal sorting of the SARS-CoV-2 host factor Neuropilin-1.
Application : WB
van Berkel AA et al. J Neurochem 2021; 157 (3) : 450-466 Loss of MUNC18-1 leads to retrograde transport defects in neurons.
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REVIEW

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Application Reference
Thosar SA et al. Cell Rep 2024; 43 (1):113656 Oxidative guanine base damage plays a dual role in regulating productive ALT-associated homology-directed repair.
Laspata N et al. Nucleic Acids Res 2023; 51 (5):2215-2237 PARP1 associates with R-loops to promote their resolution and genome stability.
Application : WB
Boris Simonetti et al. PLoS Biol 2022; 20 (4):e3001601 SNX27-Retromer directly binds ESCPE-1 to transfer cargo proteins during endosomal recycling.
Application : WB
Boris Simonetti et al. Proc Natl Acad Sci U S A 2022; 119 (25):e2201980119 ESCPE-1 mediates retrograde endosomal sorting of the SARS-CoV-2 host factor Neuropilin-1.
Application : WB
van Berkel AA et al. J Neurochem 2021; 157 (3):450-466 Loss of MUNC18-1 leads to retrograde transport defects in neurons.
Borga C et al. Leukemia 2019; 33 (2):333-347 Simultaneous B and T cell acute lymphoblastic leukemias in zebrafish driven by transgenic MYC: implications for oncogenesis and lymphopoiesis.
Magic Z et al. Int J Oncol 2019; 55 (6):1275-1286 Ephrin?2 inhibits cell proliferation and motility in?vitro and predicts longer metastasis?ree survival in breast cancer.
Zeljana Magic et al. International Journal of Oncology 2019; (Epub) Ephrin?B2 inhibits cell proliferation and motility in vitro and predicts longer metastasis?free survival in breast cancer.
Brouwer M et al. EMBO J 2019;:e101289 SALM1 controls synapse development by promoting F-actin/PIP2-dependent Neurexin clustering.
Application : ICC/IF, IP
Fouquerel E et al. Mol Cell 2019; (Epub) Targeted and Persistent 8-Oxoguanine Base Damage at Telomeres Promotes Telomere Loss and Crisis.
Scanlon DP et al. Nat Commun 2017; 8:15220 An evolutionary switch in ND2 enables Src kinase regulation of NMDA receptors.
Dominguez N et al. J Neurochem 2017; (Epub) Dense-Core Vesicle biogenesis and exocytosis in neurons lacking Chromogranins A and B.
Wallez Y et al. J Biol Chem 2014; 289 (15):10431-44 Association of the Breast Cancer Antiestrogen Resistance Protein 1 (BCAR1) and BCAR3 Scaffolding Proteins in Cell Signaling and Antiestrogen Resistance.
Application : WB, IP
Ramos-Ibeas P et al. Reproduction 2013; 146 (5):481-9 Most regions of mouse epididymis are able to phagocytose immature germ cells.
Application : ICC/IF
Bordignon V et al. PLoS One 2013; 8 (5):e64613 Production of cloned pigs with targeted attenuation of gene expression.
Application : WB
Mace PD et al. Nat Struct Mol Biol 2011; 18 (12):1381-7 NSP-Cas protein structures reveal a promiscuous interaction module in cell signaling.
Application : IP
Kramer LB et al. PLoS One 2010; 5 (12):e14291 UEV-1 is an ubiquitin-conjugating enzyme variant that regulates glutamate receptor trafficking in C. elegans neurons.
Application : WB
Liu FJ et al. PLoS One 2010; 5 (6):e11152 The Werner syndrome protein suppresses telomeric instability caused by chromium (VI) induced DNA replication stress.
Application : ICC/IF
Catalucci D et al. J Cell Biol 2009; 184 (6):923-33 Akt regulates L-type Ca2+ channel activity by modulating Cavalpha1 protein stability.
Dunlap SM et al. Proc Natl Acad Sci U S A 2007; 104 (28):11736-41 *-like growth factor binding protein 2 promotes glioma development and progression.
Application : IHC
SDS
Thimerosal.pdf
Package List Price ($)
$ 399