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GFP antibody

Anti-GFP antibody used in Western Blot (WB). GTX30266

Cat. No. GTX30266

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-Fr, IP

Reactivity

 Species independent
Package
100 μg ($399)
See all GFP products

APPLICATION

Application Note

For IP: Use at a dilution of 1:200-1:500. For WB: Use at a dilution of 1:5,000. Optimal dilutions/concentrations should be determined by the researcher.

Product Note

This antibody reacts with wild-type GFP, and itÕs variants, such as EGFP, EBFP.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.1% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

Purified protein - native GFP Aequorea victoria

Purification

Protein A purified

Conjugation

Unconjugated

RRID

AB_371419

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Green Fluorescent Protein

Background

The jellyfish Aequorea victoria contains green fluorescent protein (GFP) that emits light in the bioluminescence reaction of the animal. GFP has been used widely as a reporter protein for gene expression in eukaryotic and prokaryotic organisms, and as a protein tag in cell culture and in multicellular organisms. As a fusion tag, GFP can be used to localize proteins, to study their movement or to research the dynamics of the subcellular compartments where these proteins are targeted. GFP technology has revealed considerable new insights into the physiological activities of living cells.

Research Area

DATA IMAGES

Anti-GFP antibody used in Western Blot (WB). GTX30266

GTX30266 WB Image

Western blot detection of GFP-MIP2 fusion protein in transfectant by anti-GFP.

REFERENCE

Application Reference

Abdallah K et al. Sci Rep 2018; 8 (1) : 7321 Adeno-associated virus 2/9 delivery of Cre recombinase in mouse primary afferents.
Application : IHC-Fr
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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399