GeneTex
United States (US)

GFP antibody

GFP-antibody_WB_GTX30266-1_18121410_109.jpg

Cat No. GTX30266

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-Fr, IP
APPLICATION

Application Note

For IP: Use at a dilution of 1:200-1:500. For WB: Use at a dilution of 1:5,000. Optimal dilutions/concentrations should be determined by the researcher.

Specificity/Sensitivity

This antibody reacts with wild-type GFP, and itÕs variants, such as EGFP, EBFP.
PROPERTIES

Form

Liquid

Buffer

PBS, pH7.2 containing 0.1% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

Purified protein - native GFP Aequorea victoria

Purification

Protein A purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Green Fluorescent Protein

Background

The jellyfish Aequorea victoria contains green fluorescent protein (GFP) that emits light in the bioluminescence reaction of the animal. GFP has been used widely as a reporter protein for gene expression in eukaryotic and prokaryotic organisms, and as a protein tag in cell culture and in multicellular organisms. As a fusion tag, GFP can be used to localize proteins, to study their movement or to research the dynamics of the subcellular compartments where these proteins are targeted. GFP technology has revealed considerable new insights into the physiological activities of living cells.

Research Area

DATA IMAGES
GFP-antibody_WB_GTX30266-1_18121410_109.jpg

GTX30266 WB Image

Western blot detection of GFP-MIP2 fusion protein in transfectant by anti-GFP.

REFERENCE
Package List Price ($)
$ 319