GeneTex

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United States (US)

GFP antibody

Cat. No. GTX30738

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, FACS, IP, ELISA, ChIP assay, Gel supershift assays, IM, IHC (Free Floating)

Reactivity

 Species independent
Package
25 μl ($299)
See all GFP products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
ICC/IF Assay dependent
IHC-P Assay dependent
FACS Assay dependent
IP Assay dependent
ELISA Assay dependent
ChIP assay Assay dependent
Gel supershift assays Assay dependent
IM Assay dependent
IHC (Free Floating) Assay dependent
Not tested in other applications.

Product Note

Reactive against all variants of Aequorea victoria GFP such as S65T-GFP, RS-GFP, YFP and EGFP.

PROPERTIES

Form

Liquid

Buffer

Serum

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Aequorea victoria

Immunogen

The GFP was isolated directly from the jellyfish Aequorea victoria.

Purification

Unpurified

Conjugation

Unconjugated

RRID

AB_1240917

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Background

A highly versatile antibody that gives a stronger signal than other anti-GFP antibodies available. On Western blot the antibody detects the GFP fraction from cell extracts expressing recombinant GFP fusion proteins and has also been shown to be useful on mouse sections fixed with formalin (Mesaeli et al. 1999). In Immunocytochemistry, the antibody gives a very good signal on recombinant YES-GFP chimeras expressed in COS cells (McCabe et al. 1999 and figure below). It is routinely used in Immunoprecipitation (IP) and IP-Western protocols and has been used successfully in HRP Immunohistochemistry at 1:200 on whole-mount mouse embryos.

Research Area

REFERENCE

Application Reference

Martinez-Guzman O et al. J Cell Sci 2020; 133 (10) Mitochondrial-nuclear heme trafficking in budding yeast is regulated by GTPases that control mitochondrial dynamics and ER contact sites.
Application : WB
Osiris Martinez-Guzman et al. bioRxiv 2019; (Epub) The heme biosynthetic enzyme, 5-aminolevulinic acid synthase (ALAS), and GTPases in control of mitochondrial dynamics and ER contact sites, regulate heme mobilization to the nucleus.
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SDS
Thimerosal.pdf
Package List Price ($)
$ 299