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GGA1 antibody

Anti-GGA1 antibody used in IHC (Paraffin sections) (IHC-P). GTX114175
Anti-GGA1 antibody used in Western Blot (WB). GTX114175

Cat No. GTX114175

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P

Reactivity

Human
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

70 kDa. ( Note )

Positive Control

NT2D1 , PC-3 , U87-MG , SK-N-SH

Predict Reactivity

Mouse, Rat, Bovine, Xenopus laevis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human GGA1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11167702

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

golgi associated, gamma adaptin ear containing, ARF binding protein 1

Cellular Localization

Golgi apparatus , trans-Golgi network membrane; Peripheral membrane protein , Endosome membrane; Peripheral membrane protein

Background

This gene encodes a member of the Golgi-localized, gamma adaptin ear-containing, ARF-binding (GGA) protein family. Members of this family are ubiquitous coat proteins that regulate the trafficking of proteins between the trans-Golgi network and the lysosome. These proteins share an amino-terminal VHS domain which mediates sorting of the mannose 6-phosphate receptors at the trans-Golgi network. They also contain a carboxy-terminal region with homology to the ear domain of gamma-adaptins. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-GGA1 antibody used in IHC (Paraffin sections) (IHC-P). GTX114175

GTX114175 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human gastric cancer, using GGA1(GTX114175) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-GGA1 antibody used in Western Blot (WB). GTX114175

GTX114175 WB Image

Sample (30 ug of whole cell lysate)
A: NT2D1
B: PC-3
C: U87-MG
D: SK-N-SH
7.5% SDS PAGE
GTX114175 diluted at 1:1000

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169