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GGT1 antibody

Anti-GGT1 antibody used in Western Blot (WB). GTX101198
Anti-GGT1 antibody used in Western Blot (WB). GTX101198
Anti-GGT1 antibody used in IHC (Paraffin sections) (IHC-P). GTX101198

Cat. No. GTX101198

Reference 5

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all GGT1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

61 kDa. ( Note )

Positive Control

Neuro2A , GL261 , C8D30 , NIH-3T3 , Raw264.7 , C2C12

Product Note

Based on sequence homology, this antibody may cross react with GGT2 protein.

Predict Reactivity

Rat, Bovine, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.71 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the Extracellular domain of human GGT1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950379

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

gamma-glutamyltransferase 1 , CD224 , D22S672 , D22S732 , GGT , GGT 1 , GGTD , GTG

Cellular Localization

Membrane; Single-pass type II membrane protein

Background

The enzyme encoded by this gene catalyzes the transfer of the glutamyl moiety of glutathione to a variety of amino acids and dipeptide acceptors. The enzyme is composed of a heavy chain and a light chain, which are derived from a single precursor protein, and is present in tissues involved in absorption and secretion. This enzyme is a member of the gamma-glutamyltransferase protein family, of which many members have not yet been fully characterized and some of which may represent pseudogenes. This gene is classified as type I gamma-glutamyltransferase. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-GGT1 antibody used in Western Blot (WB). GTX101198

GTX101198 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GGT1 antibody (GTX101198) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-GGT1 antibody used in Western Blot (WB). GTX101198

GTX101198 WB Image

GGT1 antibody detects GGT1 protein by western blot analysis.
A. 30 μg Neuro2A whole cell lysate/extract
B. 30 μg GL261 whole cell lysate/extract
C. 30 μg C8D30 whole cell lysate/extract
D. 30 μg NIH-3T3 whole cell lysate/extract
E. 30 μg Raw264.7 whole cell lysate/extract
F. 30 μg C2C12 whole cell lysate/extract
7.5% SDS-PAGE
GGT1 antibody (GTX101198) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-GGT1 antibody used in IHC (Paraffin sections) (IHC-P). GTX101198

GTX101198 IHC-P Image

GGT1 antibody detects GGT1 protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
GGT1 stained by GGT1 antibody (GTX101198) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

Application Reference

Iyama T et al. Sci Rep 2020; 10 (1) : 12094 A novel method for assessing the renal biopsy specimens using an activatable fluorescent probe.
Application : IHC-P
Reactivity : Human
Tiu AC et al. FASEB J 2020; 34 (5) : 6999-7017 Lipid rafts are required for effective renal D 1 dopamine receptor function.
Application : WB
Reactivity : Human
Zhou Q et al. Nat Nanotechnol 2019; (Epub) Enzyme-activatable polymer-drug conjugate augments tumour penetration and treatment efficacy.
Application : IHC-P
Reactivity : Mouse
Maclean KN et al. FASEB J 2017; (Epub) Taurine treatment prevents derangement of the hepatic γ-glutamyl cycle and methylglyoxal metabolism in a mouse model of classical homocystinuria: regulatory crosstalk between thiol and sulfinic acid metabolism.
Application : WB
Reactivity : Mouse
Tobe R et al. Cancers (Basel) 2015; 7 (4) : 2262-76 Differences in Redox Regulatory Systems in Human Lung and Liver Tumors Suggest Different Avenues for Therapy.
Application : WB
Reactivity : Human
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169