GeneTex
United States (US)

GGT1 antibody

GTX101198_39932_WB_M_19011506_631.jpg
GTX101198_WB_19011506_270.jpg
GTX101198_39932_IHC_19011506_108.jpg

Cat No. GTX101198

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

61 kDa. ( Note )

Positive Control

Molt-4 , Neuro2A , GL261 , C8D30 , NIH-3T3 , Raw264.7 , C2C12

Predict Reactivity

Rat, Bovine, Pig(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human GGT1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Gamma-Glutamyltransferase 1,Cd224,D22S672,D22S732,Ggt,Ggt 1,Ggtd,Gtg,Ggt1

Cellular Localization

Membrane; Single-pass type II membrane protein

Background

The enzyme encoded by this gene catalyzes the transfer of the glutamyl moiety of glutathione to a variety of amino acids and dipeptide acceptors. The enzyme is composed of a heavy chain and a light chain, which are derived from a single precursor protein, and is present in tissues involved in absorption and secretion. This enzyme is a member of the gamma-glutamyltransferase protein family, of which many members have not yet been fully characterized and some of which may represent pseudogenes. This gene is classified as type I gamma-glutamyltransferase. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX101198_39932_WB_M_19011506_631.jpg

GTX101198 WB Image

GGT1 antibody detects GGT1 protein by western blot analysis.
A. 30 µg Neuro2A whole cell lysate/extract
B. 30 µg GL261 whole cell lysate/extract
C. 30 µg C8D30 whole cell lysate/extract
D. 30 µg NIH-3T3 whole cell lysate/extract
E. 30 µg Raw264.7 whole cell lysate/extract
F. 30 µg C2C12 whole cell lysate/extract
7.5% SDS-PAGE
GGT1 antibody (GTX101198) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX101198_WB_19011506_270.jpg

GTX101198 WB Image

Sample(30 µg of whole cell lysate)
A:MOLT4(GTX27912)
7.5% SDS PAGE
GTX101198 diluted at 1:500
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX101198_39932_IHC_19011506_108.jpg

GTX101198 IHC-P Image

Immunohistochemical analysis of paraffin-embedded PC13 xenograft, using GGT1(GTX101198) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169