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GGT5 antibody, N-term

Anti-GGT5 antibody, N-term used in Western Blot (WB). GTX81477
Anti-GGT5 antibody, N-term used in Flow cytometry (FACS). GTX81477
Anti-GGT5 antibody, N-term used in Western Blot (WB). GTX81477
Anti-GGT5 antibody, N-term used in IHC (Paraffin sections) (IHC-P). GTX81477

Cat. No. GTX81477

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, FACS

Reactivity

 Human, Mouse
Package
400 μl ($399)
See all GGT5 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000
IHC-P 1:50-1:100
FACS 1:10-1:50
Not tested in other applications.

Calculated MW

62 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

KLH conjugated synthetic peptide between 130-156 amino acids from the N-terminal region of human GGTLA1.

Purification

Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

gamma-glutamyltransferase 5 , GGL , GGT 5 , GGT-REL , GGTLA1

Cellular Localization

Membrane

Background

This gene is a member of the gamma-glutamyl transpeptidase gene family, and some reports indicate that it is capable of cleaving the gamma-glutamyl moiety of glutathione. The protein encoded by this gene is synthesized as a single, catalytically-inactive polypeptide, that is processed post-transcriptionally to form a heavy and light subunit, with the catalytic activity contained within the small subunit. The encoded enzyme is able to convert leukotriene C4 to leukotriene D4, but appears to have distinct substrate specificity compared to gamma-glutamyl transpeptidase. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Oct 2014]

Database

Research Area

DATA IMAGES

Anti-GGT5 antibody, N-term used in Western Blot (WB). GTX81477

GTX81477 WB Image

WB analysis of mouse heart tissue lysate (35ug/lane) using GTX81477 GGT5 antibody, N-term.

Anti-GGT5 antibody, N-term used in Flow cytometry (FACS). GTX81477

GTX81477 FACS Image

FACS analysis of HepG2 cells using GTX81477 GGT5 antibody, N-term.
Top histogram : negative control
Bottom histogram : HepG2 cells

Anti-GGT5 antibody, N-term used in Western Blot (WB). GTX81477

GTX81477 WB Image

WB analysis of Y79 cell lysate (35ug/lane) using GTX81477 GGT5 antibody, N-term.

Anti-GGT5 antibody, N-term used in IHC (Paraffin sections) (IHC-P). GTX81477

GTX81477 IHC-P Image

IHC-P analysis of human brain tissue using GTX81477 GGT5 antibody, N-term.

REFERENCE

Application Reference

Passarelli MC et al. Nat Cell Biol 2022; 24 (3) : 307-315 Leucyl-tRNA synthetase is a tumour suppressor in breast cancer and regulates codon-dependent translation dynamics.
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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399