GeneTex
United States (US)

GHS-R1 antibody [N1], N-term

GTX108171_39735_20170706_WB_19011507_926.jpg
GTX108171_39735_20170706_WB_M_R_19011507_446.jpg
GTX108171_39735_IHC_19011507_168.jpg
GTX108171_39735_20170706_WB_M_hypothalamus_19011507_322.jpg

Cat No. GTX108171

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

41 kDa. ( Note )

Positive Control

NT2D1 , PC-3 , U87-MG , SK-N-SH , mouse hypothalamus , mouse brain , rat brain

Predict Reactivity

Pig(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human GHS-R1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

GHSR antibody, growth hormone secretagogue receptor type 1 antibody, GH-releasing peptide receptor antibody, ghrelin receptor antibody, GHRP antibody, GHS-R antibody, growth hormone secretagogue receptor antibody

Cellular Localization

Cell membrane; Multi-pass membrane protein

Background

This gene encodes a member of the G-protein coupled receptor family. The encoded protein may play a role in energy homeostasis and regulation of body weight. Two identified transcript variants are expressed in several tissues and are evolutionary conserved in fish and swine. One transcript, 1a, excises an intron and encodes the functional protein; this protein is the receptor for the Ghrelin ligand and defines a neuroendocrine pathway for growth hormone release. The second transcript (1b) retains the intron and does not function as a receptor for Ghrelin; however, it may function to attenuate activity of isoform 1a. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX108171_39735_20170706_WB_19011507_926.jpg

GTX108171 WB Image

Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with GHS-R1 antibody [N1], N-term (GTX108171) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX108171_39735_20170706_WB_M_R_19011507_446.jpg

GTX108171 WB Image

Various tissue extracts (50 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with GHS-R1 antibody [N1], N-term (GTX108171) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX108171_39735_IHC_19011507_168.jpg

GTX108171 IHC-P Image

Immunohistochemical analysis of paraffin-embedded CL1-0 xenograft, using GHS-R1(GTX108171) antibody at 1:100 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX108171_39735_20170706_WB_M_hypothalamus_19011507_322.jpg

GTX108171 WB Image

Mouse tissue extract (50 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with GHS-R1 antibody [N1], N-term (GTX108171) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169