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GHS-R1 antibody [N1], N-term

KO/KD Orthogonal
Anti-GHS-R1 antibody [N1], N-term used in Western Blot (WB). GTX108171
Anti-GHS-R1 antibody [N1], N-term used in Western Blot (WB). GTX108171
Anti-GHS-R1 antibody [N1], N-term used in IHC (Paraffin sections) (IHC-P). GTX108171
Anti-GHS-R1 antibody [N1], N-term used in Western Blot (WB). GTX108171

Cat. No. GTX108171

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all GHS-R1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

41 kDa. ( Note )

Positive Control

NT2D1 , PC-3 , U87-MG , SK-N-SH , mouse hypothalamus , mouse brain , rat brain

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide encompassing a sequence within the Extracellular domain of human GHS-R1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950387

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

growth hormone secretagogue receptor , GHDP

Cellular Localization

Cell membrane; Multi-pass membrane protein

Background

This gene encodes a member of the G-protein coupled receptor family. The encoded protein may play a role in energy homeostasis and regulation of body weight. Two identified transcript variants are expressed in several tissues and are evolutionary conserved in fish and swine. One transcript, 1a, excises an intron and encodes the functional protein; this protein is the receptor for the Ghrelin ligand and defines a neuroendocrine pathway for growth hormone release. The second transcript (1b) retains the intron and does not function as a receptor for Ghrelin; however, it may function to attenuate activity of isoform 1a. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-GHS-R1 antibody [N1], N-term used in Western Blot (WB). GTX108171

GTX108171 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GHS-R1 antibody [N1], N-term (GTX108171) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-GHS-R1 antibody [N1], N-term used in Western Blot (WB). GTX108171

GTX108171 WB Image

Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GHS-R1 antibody [N1], N-term (GTX108171) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-GHS-R1 antibody [N1], N-term used in IHC (Paraffin sections) (IHC-P). GTX108171

GTX108171 IHC-P Image

Immunohistochemical analysis of paraffin-embedded CL1-0 xenograft, using GHS-R1(GTX108171) antibody at 1:100 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-GHS-R1 antibody [N1], N-term used in Western Blot (WB). GTX108171

GTX108171 WB Image

Mouse tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with GHS-R1 antibody [N1], N-term (GTX108171) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Chia-Hao Wang et al. Biomolecules 2022; 12 (12) Establishment of a Cell Line Stably Expressing the Growth Hormone Secretagogue Receptor to Identify Crocin as a Ghrelin Agonist
Application : WB, ICC/IF
Reactivity : Human
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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169