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GILT antibody

Anti-GILT antibody used in Western Blot (WB). GTX103967
Anti-GILT antibody used in IHC (Paraffin sections) (IHC-P). GTX103967

Cat. No. GTX103967

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all GILT products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

28 kDa. ( Note )

Positive Control

Raji

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human GILT. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950545

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

IFI30 lysosomal thiol reductase , GILT , IFI-30 , IP-30 , IP30

Background

The protein encoded by this gene is a lysosomal thiol reductase that at low pH can reduce protein disulfide bonds. The enzyme is expressed constitutively in antigen-presenting cells and induced by gamma-interferon in other cell types. This enzyme has an important role in MHC class II-restricted antigen processing. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-GILT antibody used in Western Blot (WB). GTX103967

GTX103967 WB Image

Sample(30 ug whole cell lysate)
A:Raji (GTX27908)
12% SDS PAGE
GTX103967 diluted at 1:1000

Anti-GILT antibody used in IHC (Paraffin sections) (IHC-P). GTX103967

GTX103967 IHC-P Image

GILT antibody detects IFI30 protein at cytosol on AGS xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded AGS xenograft.
GILT antibody (GTX103967) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

Application Reference

Chuang YH et al. Int J Mol Sci 2023; 24 (17) Identification of the HNSC88 Molecular Signature for Predicting Subtypes of Head and Neck Cancer.
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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169