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GILZ antibody

Anti-GILZ antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX33560
Anti-GILZ antibody used in IHC (Paraffin sections) (IHC-P). GTX33560
Anti-GILZ antibody used in Immunoprecipitation (IP). GTX33560
Anti-GILZ antibody used in Western Blot (WB). GTX33560

Cat. No. GTX33560

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP

Reactivity

 Human, Mouse
Package
100 μl ($399)
See all GILZ products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
IHC-P 1:50 - 1:200
IP 1:50 - 1:100
Not tested in other applications.

Calculated MW

15 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-79 of human TSC22D3 (NP_001015881.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

TSC22 domain family member 3 , DIP , DSIPI , GILZ , TSC-22R

Cellular Localization

Nucleus

Background

This gene encodes the anti-inflammatory protein glucocorticoid (GC)-induced leucine zipper. Expression of this gene stimulated by glucocorticoids and interleukin 10 and it appears to play a key role in the anti-inflammatory and immunosuppressive effects of this steroid. This protein has also been shown to inhibit pro-inflammatory molecules including nuclear factor κB. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jan 2016]

Database

Research Area

DATA IMAGES

Anti-GILZ antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX33560

GTX33560 ICC/IF Image

ICC/IF analysis of U2OS cells using GTX33560 GILZ antibody.
Blue : DAPI

Anti-GILZ antibody used in IHC (Paraffin sections) (IHC-P). GTX33560

GTX33560 IHC-P Image

IHC-P analysis of mouse lung tissue using GTX33560 GILZ antibody.
Dilution : 1:100

Anti-GILZ antibody used in Immunoprecipitation (IP). GTX33560

GTX33560 IP Image

IP analysis of 293T cell lysate using GTX33560 GILZ antibody.
Antibody amount : 3μg / 200μg lysate
Dilution : 1:1000

Anti-GILZ antibody used in Western Blot (WB). GTX33560

GTX33560 WB Image

WB analysis of various sample lysates using GTX33560 GILZ antibody.
Dilution : 1:1000
Loading : 25μg per lane

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399