GeneTex
United States (US)

GIT1 antibody [N3C2], Internal

GTX105824_39862_20170511_WB_M_R_19011506_752.jpg
GTX105824_39862_20160428_WB_19011506_618.jpg
GTX105824_39862_WB_R_19011506_803.jpg
GTX105824_39862_IFA_19011506_658.jpg
GTX105824_IHC_19011506_727.jpg
GTX105824_WB_19011506_725.jpg

Cat No. GTX105824

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

84 kDa. ( Note )

Positive Control

HepG2 , Rat2 , HUVEC , mouse brain , rat brain

Predict Reactivity

Bovine(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.65mg/ml

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human GIT1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

GIT1 antibody, CAT-1 antibody, CAT1 antibody, cool-associated and tyrosine-phosphorylated protein 1 antibody, G protein-coupled receptor kinase-interactor 1 antibody, G protein-coupled receptor kinase interactor 1 antibody, GRK-interacting protein 1 antibody, ARF GTPase-activating protein GIT1 antibody, ARF GAP GIT1 antibody, G protein-coupled receptor kinase interacting ArfGAP 1 antibody

Cellular Localization

Cytoplasm

Database

Research Area

DATA IMAGES
GTX105824_39862_20170511_WB_M_R_19011506_752.jpg

GTX105824 WB Image

Various tissue extracts (50 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GIT1 antibody (GTX105824) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX105824_39862_20160428_WB_19011506_618.jpg

GTX105824 WB Image

Whole cell extract (30 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with GIT1 antibody [N3C2], Internal (GTX105824) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX105824_39862_WB_R_19011506_803.jpg

GTX105824 WB Image

GIT1 antibody [N3C2], Internal detects GIT1 protein by western blot analysis.
A. 30 µg Rat2 whole cell lysate/extract
7.5% SDS-PAGE
GIT1 antibody [N3C2], Internal (GTX105824) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX105824_39862_IFA_19011506_658.jpg

GTX105824 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using GIT1(GTX105824) antibody at 1:500 dilution.

GTX105824_IHC_19011506_727.jpg

GTX105824 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human mixed ovarian cancer, using GIT1(GTX105824) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX105824_WB_19011506_725.jpg

GTX105824 WB Image

Sample(30 µg of whole cell lysate)
A:Hep G2(GTX27900)
7.5% SDS PAGE
GTX105824 diluted at 1:2000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Reference
SDS
Glycerol.pdf
Thimerosal.pdf