Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GIT1 antibody (GTX105824) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with GIT1 antibody [N3C2], Internal (GTX105824) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data are based on Human Protein Atlas program.

Immunofluorescence analysis of methanol-fixed HeLa, using GIT1(GTX105824) antibody at 1:500 dilution.

GIT1 antibody [N3C2], Internal detects GIT1 protein by western blot analysis.
A. 30 μg Rat2 whole cell lysate/extract
7.5% SDS-PAGE
GIT1 antibody [N3C2], Internal (GTX105824) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunohistochemical analysis of paraffin-embedded human mixed ovarian cancer, using GIT1(GTX105824) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Sample(30 μg of whole cell lysate)
A:Hep G2(GTX27900)
7.5% SDS PAGE
GTX105824 diluted at 1:2000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.