GeneTex

  • Country / Location Selection

United States (US)

GIT1 antibody

Anti-GIT1 antibody used in Western Blot (WB). GTX133292
Anti-GIT1 antibody used in Western Blot (WB). GTX133292
Anti-GIT1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133292
Anti-GIT1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133292

Cat. No. GTX133292

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all GIT1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

85 kDa. ( Note )

Positive Control

mouse brain , rat brain , A549 , H1299 , HCT116

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.76 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Recombinant protein encompassing a sequence within the center region of mouse GIT1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886914

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

GIT ArfGAP 1 , Cat-1 , p95Cat

Cellular Localization

Cytoplasm

Background

GTPase-activating protein for the ADP ribosylation factor family. May serve as a scaffold to bring together molecules to form signaling modules controlling vesicle trafficking, adhesion and cytoskeletal organization. Increases the speed of cell migration, as well as the size and rate of formation of protrusions, possibly by targeting PAK1 to adhesions and the leading edge of lamellipodia. Sequesters inactive non-tyrosine-phosphorylated paxillin in cytoplasmic complexes.

Database

Research Area

DATA IMAGES

Anti-GIT1 antibody used in Western Blot (WB). GTX133292

GTX133292 WB Image

Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GIT1 antibody (GTX133292) diluted at 1:1000.

Anti-GIT1 antibody used in Western Blot (WB). GTX133292

GTX133292 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GIT1 antibody (GTX133292) diluted at 1:1000.

Anti-GIT1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133292

GTX133292 ICC/IF Image

GIT1 antibody detects GIT1 protein by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: GIT1 stained by GIT1 antibody (GTX133292) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (GTX631831) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-GIT1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133292

GTX133292 ICC/IF Image

GIT1 antibody detects GIT1 protein at cytoplasm by immunofluorescent analysis.
Sample: HCT 116 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: GIT1 protein stained by GIT1 antibody (GTX133292) diluted at 1:500.
Blue: Hoechst 33342 staining.

REFERENCE

There are currently no references for GIT1 antibody (GTX133292). Be the first to share your publications with this product.
Submit a Reference

REVIEW

There are currently no reviews for GIT1 antibody (GTX133292). Be the first to share your experience with this product.
Submit a Review
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169