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GIT2 antibody [N1N3]

Anti-GIT2 antibody [N1N3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX116427
Anti-GIT2 antibody [N1N3] used in Western Blot (WB). GTX116427
Anti-GIT2 antibody [N1N3] used in Western Blot (WB). GTX116427
Anti-GIT2 antibody [N1N3] used in IHC (Frozen sections) (IHC-Fr). GTX116427

Cat No. GTX116427

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-Fr

Reactivity

Human, Mouse
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-Fr 1:100-1:1000
Not tested in other applications.

Calculated MW

85 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , mouse liver

Predict Reactivity

Rat, Bovine, Chicken, Xenopus laevis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris-Glycine (pH7), 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.81 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human GIT2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11178590

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

GIT ArfGAP 2 , CAT-2 , CAT2 , PKL

Background

This gene encodes a member of the GIT protein family, which interact with G protein-coupled receptor kinases and possess ADP-ribosylation factor (ARF) GTPase-activating protein (GAP) activity. GIT proteins traffic between cytoplasmic complexes, focal adhesions, and the cell periphery, and interact with Pak interacting exchange factor beta (PIX) to form large oligomeric complexes that transiently recruit other proteins. GIT proteins regulate cytoskeletal dynamics and participate in receptor internalization and membrane trafficking. This gene has been shown to repress lamellipodial extension and focal adhesion turnover, and is thought to regulate cell motility. This gene undergoes extensive alternative splicing to generate multiple isoforms, but the full-length nature of some of these variants has not been determined. The various isoforms have functional differences, with respect to ARF GAP activity and to G protein-coupled receptor kinase 2 binding. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-GIT2 antibody [N1N3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX116427

GTX116427 ICC/IF Image

GIT2 antibody [N1N3] detects GIT2 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: GIT2 protein stained by GIT2 antibody [N1N3] (GTX116427) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-GIT2 antibody [N1N3] used in Western Blot (WB). GTX116427

GTX116427 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GIT2 antibody [N1N3] (GTX116427) diluted at 1:1000.

Anti-GIT2 antibody [N1N3] used in Western Blot (WB). GTX116427

GTX116427 WB Image

Mouse tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with GIT2 antibody [N1N3] (GTX116427) diluted at 1:1000.

Anti-GIT2 antibody [N1N3] used in IHC (Frozen sections) (IHC-Fr). GTX116427

GTX116427 IHC-Fr Image

GIT2 antibody [N1N3] detects GIT2 protein expression by immunohistochemical analysis.
Sample: Frozen-sectioned adult mouse cerebellum.
Green: GIT2 protein stained by GIT2 antibody [N1N3] (GTX116427) diluted at 1:250.
Red: NF-H, stained by NF-H antibody [GT114] (GTX634289) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Antigen Retrieval: Citrate buffer, pH 6.0, 10 min

REFERENCE

There are currently no references for GIT2 antibody [N1N3] (GTX116427). Be the first to share your publications with this product.

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169