APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| 1:100-1:1000 |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
293T , A431 , HeLa , HepG2 , mouse liver
Predict Reactivity
Rat, Bovine, Chicken, Xenopus tropicalis, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
0.1M Tris, 0.1M Glycine, 20% Glycerol
Preservative
0.01% Thimerosal
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.81 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human GIT2. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_11178590
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
GIT ArfGAP 2 , CAT-2 , CAT2 , PKL
Background
This gene encodes a member of the GIT protein family, which interact with G protein-coupled receptor kinases and possess ADP-ribosylation factor (ARF) GTPase-activating protein (GAP) activity. GIT proteins traffic between cytoplasmic complexes, focal adhesions, and the cell periphery, and interact with Pak interacting exchange factor beta (PIX) to form large oligomeric complexes that transiently recruit other proteins. GIT proteins regulate cytoskeletal dynamics and participate in receptor internalization and membrane trafficking. This gene has been shown to repress lamellipodial extension and focal adhesion turnover, and is thought to regulate cell motility. This gene undergoes extensive alternative splicing to generate multiple isoforms, but the full-length nature of some of these variants has not been determined. The various isoforms have functional differences, with respect to ARF GAP activity and to G protein-coupled receptor kinase 2 binding. [provided by RefSeq]
Database
Research Area
DATA IMAGES
![Anti-GIT2 antibody [N1N3] used in Western Blot (WB). GTX116427](/templates/default/js/lightbox/images/loading.gif "Anti-GIT2 antibody [N1N3] used in Western Blot (WB). GTX116427")
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GTX116427 WB Image
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GIT2 antibody [N1N3] (GTX116427) diluted at 1:1000.
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GTX116427 IHC-Fr Image
GIT2 antibody [N1N3] detects GIT2 protein expression by immunohistochemical analysis.
Sample: Frozen-sectioned adult mouse cerebellum.
Green: GIT2 protein stained by GIT2 antibody [N1N3] (GTX116427) diluted at 1:250.
Red: NF-H, stained by NF-H antibody [GT114] (GTX634289) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 10 min
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GTX116427 WB Image
Mouse tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with GIT2 antibody [N1N3] (GTX116427) diluted at 1:1000.
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GTX116427 ICC/IF Image
GIT2 antibody [N1N3] detects GIT2 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: GIT2 protein stained by GIT2 antibody [N1N3] (GTX116427) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.
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REFERENCE
There are currently no references for GIT2 antibody [N1N3] (GTX116427). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for GIT2 antibody [N1N3] (GTX116427). Be the first to share your experience with this product.
![Anti-GIT2 antibody [N1N3] used in Western Blot (WB). GTX116427](/upload/website/prouct_img/small/GTX116427/GTX116427_40639_20170209_WB_w_23060519_303.webp "Anti-GIT2 antibody [N1N3] used in Western Blot (WB). GTX116427")
![Anti-GIT2 antibody [N1N3] used in IHC (Frozen sections) (IHC-Fr). GTX116427](/upload/website/prouct_img/small/GTX116427/GTX116427_40639_20170831_IHC-Fr_M_w_23060519_792.webp "Anti-GIT2 antibody [N1N3] used in IHC (Frozen sections) (IHC-Fr). GTX116427")
![Anti-GIT2 antibody [N1N3] used in Western Blot (WB). GTX116427](/upload/website/prouct_img/small/GTX116427/GTX116427_40639_20170209_WB_M_liver_w_23060519_769.webp "Anti-GIT2 antibody [N1N3] used in Western Blot (WB). GTX116427")
![Anti-GIT2 antibody [N1N3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX116427](/upload/website/prouct_img/small/GTX116427/GTX116427_40639_IFA_w_23060519_793.webp "Anti-GIT2 antibody [N1N3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX116427")
![Anti-GIT2 antibody [N1N3] used in Western Blot (WB). GTX116427](/upload/website/prouct_img/normal/GTX116427/GTX116427_40639_20170209_WB_w_23060519_303.webp "Anti-GIT2 antibody [N1N3] used in Western Blot (WB). GTX116427")
![Anti-GIT2 antibody [N1N3] used in IHC (Frozen sections) (IHC-Fr). GTX116427](/upload/website/prouct_img/normal/GTX116427/GTX116427_40639_20170831_IHC-Fr_M_w_23060519_792.webp "Anti-GIT2 antibody [N1N3] used in IHC (Frozen sections) (IHC-Fr). GTX116427")
![Anti-GIT2 antibody [N1N3] used in Western Blot (WB). GTX116427](/upload/website/prouct_img/normal/GTX116427/GTX116427_40639_20170209_WB_M_liver_w_23060519_769.webp "Anti-GIT2 antibody [N1N3] used in Western Blot (WB). GTX116427")
![Anti-GIT2 antibody [N1N3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX116427](/upload/website/prouct_img/normal/GTX116427/GTX116427_40639_IFA_w_23060519_793.webp "Anti-GIT2 antibody [N1N3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX116427")