APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1 μg/mL |
| 10 μg/mL |
| Assay dependent |
Not tested in other applications.
Calculated MW
Positive Control
THP-1 Cell Slide(GTX25540) , THP-1 Cell Lysate(GTX27913)
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
GITRL antibody was raised against purified recombinant human GITR ligand.
Purification
Protein A purified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
TNF superfamily member 18 , AITRL , GITRL , TL6 , TNLG2A , hGITRL
Cellular Localization
Cell membrane
Background
The tumor necrosis factor (TNF) and TNF receptor (TNFR) gene superfamilies regulate numerous biological functions including cell proliferation, differentiation, and survival through regulating the activation of the transcription factor NF-kappaB and various mitogen-activated protein kinases (reviewed in 1). The glucocorticoid-induced tumor necrosis factor receptor (GITR) is an emerging member of this family that is expressed on CD4+ CD25+ regulatory T cells and appears to have crucial immune regulation functions (2,3). Its ligand GITRL is expressed in endothelial and antigen-presenting cells (4) and can activate NF-kappaB, induce both pro- and anti-apoptotic effects, inhibit the suppressive activity of regulatory T cells, and co-stimulate responder T cells through GITR (5). Dominant negative forms of NIK and TRAF2 expressed in transfected 293 cells substantially inhibited NF-kappaB activation, suggesting that the GITRL-GITR pathway involves both NIK and TRAF2 (4).
Database
Research Area
DATA IMAGES
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GTX85076 WB Image
WB analysis of (A) 5 ng, (B) 25 ng, and (C) 50 ng purified recombinant GITRL protein using GTX85076 GITRL antibody.
Working concentration : 1 μg/ml
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GTX85076 ICC/IF Image
ICC/IF analysis of THP-1 cells using GTX85076 GITRL antibody.
Working concentration : 10 μg/ml
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REFERENCE
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REVIEW
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