APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:2000 |
| 1:50-1:200 |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
PBS, 50% Glycerol
Preservative
0.01% thiomersal
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
Batch dependent (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 800-900 of human GLI3 (NP_000159.3).
Purification
Purified by affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
GLI family zinc finger 3 , ACLS , GCPS , GLI3-190 , GLI3FL , PAP-A , PAPA , PAPA1 , PAPB , PHS , PPDIV
Cellular Localization
Nucleus , Cytoplasm , Cell projection, cilium
Background
This gene encodes a protein which belongs to the C2H2-type zinc finger proteins subclass of the Gli family. They are characterized as DNA-binding transcription factors and are mediators of Sonic hedgehog (Shh) signaling. The protein encoded by this gene localizes in the cytoplasm and activates patched Drosophila homolog (PTCH) gene expression. It is also thought to play a role during embryogenesis. Mutations in this gene have been associated with several diseases, including Greig cephalopolysyndactyly syndrome, Pallister-Hall syndrome, preaxial polydactyly type IV, and postaxial polydactyly types A1 and B. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
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GTX04242 WB Image
WB analysis of various tissue lysates using GTX0424 GLI3 antibody.
Loading : 25 μg
Dilution : 1:1000
Blocking buffer: 3% nonfat dry milk in TBST
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GTX04242 IHC-P Image
IHC-P analysis of rat brain tissue using GTX0424 GLI3 antibody.
Antigen retireval : Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0.
Dilution : 1:100
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GTX04242 IHC-P Image
IHC-P analysis of rat ovary tissue using GTX0424 GLI3 antibody.
Antigen retireval : Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0.
Dilution : 1:100
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GTX04242 IHC-P Image
IHC-P analysis of mouse liver tissue using GTX0424 GLI3 antibody.
Antigen retireval : Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0.
Dilution : 1:100
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GTX04242 IHC-P Image
IHC-P analysis of human colon using GTX0424 GLI3 antibody.
Antigen retireval : Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0.
Dilution : 1:100
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REFERENCE
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REVIEW
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