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GLI3 antibody

Overexpression
Anti-GLI3 antibody used in Western Blot (WB). GTX26050
Anti-GLI3 antibody used in IHC (Paraffin sections) (IHC-P). GTX26050
Anti-GLI3 antibody used in IHC (Paraffin sections) (IHC-P). GTX26050
Anti-GLI3 antibody used in Western Blot (WB). GTX26050
Anti-GLI3 antibody used in Western Blot (WB). GTX26050
Anti-GLI3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX26050

Cat. No. GTX26050

Reference 4

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, ELISA, Multiplexing

Reactivity

 Human, Fish
Package
50 μg ($399)
See all GLI3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:2000
ICC/IF Assay dependent
IHC-P 0.5-5 μg/mL
ELISA 1:6000-1:30000
Multiplexing Assay dependent
Not tested in other applications.

Calculated MW

170 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

20mM Potassium Phosphate, 150mM NaCl

Preservative

0.01% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide corresponding to an internal region near amino acids 30-60 of human Gli-3 protein.

Purification

Purified by antigen-affinity chromatography.
From serum

Conjugation

Unconjugated

RRID

AB_425213

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

GLI family zinc finger 3 , ACLS , GCPS , GLI3-190 , GLI3FL , PAP-A , PAPA , PAPA1 , PAPB , PHS , PPDIV

Cellular Localization

Nucleus,Cytoplasm

Background

Gli3 is belongs to the GLI C2H2-type zinc-finger protein family and contains 5 C2H2-type zinc fingers. Gli3 is very important for normal limb and brain development and is implicated in the transduction of Shh signal. Gli3 is also expressed in a wide variety of normal adult tissues, including lung, colon, spleen, placenta, testis, and myometrium. Defects in GLI3 are the cause of Greig cephalo-poly-syndactyly syndrome (GCPS); an autosomal dominant disorder affecting limb and craniofacial development.

Database

Research Area

DATA IMAGES

Anti-GLI3 antibody used in Western Blot (WB). GTX26050

GTX26050 WB Image

Western Blot of Rabbit anti-Gli-3 antibody. Lane 1: 50 kDa molecular weight marker. Lane 2: 293T cells transfected with CrkL-Flag. Lane 3: 293T cells transfected with human Gli-3. Load: 35 μg per lane. Primary antibody: Gli-3 antibody at 1:400 for overnight at 4ºC. Secondary antibody: IRDye800™ rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4ºC. Predicted/Observed size: 170-190 kDa for hGli-3. Other band(s): Non specific background ~60kDa.

Anti-GLI3 antibody used in IHC (Paraffin sections) (IHC-P). GTX26050

GTX26050 IHC-P Image

Immunohistochemistry of Rabbit anti-Gli-3 antibody (GTX26050). This image tissue: human glioblastoma. Specific staining was also noted in tissue from adrenal, brain, glioblastoma, colon, heart, kidney, lung, liver, skeletal muscle, ovary, pancreas, placenta, skin, spleen, stomach, testes, thymus, thyroid, tonsil and uterus. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: Gli-3 antibody at 0.625 μg/ml for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: Gli-3 is nuclear and smooth muscle. Staining: Gli-3 as precipitated red signal with hematoxylin purple nuclear counterstain.

Anti-GLI3 antibody used in IHC (Paraffin sections) (IHC-P). GTX26050

GTX26050 IHC-P Image

IHC-P analysis of human glioblastoma tissue using GTX26050 GLI3 antibody.
Dilution : 0.625 μg/mL

Anti-GLI3 antibody used in Western Blot (WB). GTX26050

GTX26050 WB Image

Overexpression

WB analysis of non-transfected and transfected 293T whole cell lysate using GTX26050 GLI3 antibody.
Loading : 35 μg
Dilution : 1:400

Anti-GLI3 antibody used in Western Blot (WB). GTX26050

GTX26050 WB Image

WB analysis of various samples using GTX26050 GLI3 antibody.
Lane 1 : Human brain tissue lysate
Lane 2 : Human lung tissue lysate
Lane 3 : Human spleen tissue lysate
Lane 4 : Mouse brain tissue lysate
Lane 5 : Mouse lung tissue lysate
Loading : 20 μg
Dilution : 1:500

Anti-GLI3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX26050

GTX26050 ICC/IF Image

ICC/IF analysis of MCF-7 cells using GTX26050 GLI3 antibody.
Red : Primary antibody
Green : alpha Tubulin
Dilution : 5 μg/mL

REFERENCE

Application Reference

Li N et al. Oncogene 2018; (Epub) Non-canonical activation of hedgehog in prostate cancer cells mediated by the interaction of transcriptionally active androgen receptor proteins with Gli3.
Application : WB
Reactivity : Human
Bloomquist, Ryan F et al. 0 2015; Developmental plasticity of stem cells in teeth and taste bud renewal.
Application : IHC-P
Reactivity : Fish
Hunt R et al. Hybridoma (Larchmt) 2007; 26 (4) : 231-40 Generation and characterization of mouse monoclonal antibody 5E1 against human transcription factor GLI3.
Hunt R et al. Hybridoma (Larchmt) 2007; 26 (3) : 131-9 Generation and characterization of mouse monoclonal antibody 5E1 against human transcription factor GLI3.
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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399