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GLMN antibody

Anti-GLMN antibody used in IHC (Paraffin sections) (IHC-P). GTX103064
Anti-GLMN antibody used in Western Blot (WB). GTX103064
Anti-GLMN antibody used in IHC (Paraffin sections) (IHC-P). GTX103064
Anti-GLMN antibody used in Western Blot (WB). GTX103064
Anti-GLMN antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103064

Cat. No. GTX103064

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all GLMN products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:5000-1:20000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

68 kDa. ( Note )

Positive Control

NIH-3T3 , Jurkat , Raji , NCI-H929

Predict Reactivity

Bovine, Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.23 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human GLMN. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11169732

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

glomulin, FKBP associated protein , FAP , FAP48 , FAP68 , FKBPAP , GLML , GVM , VMGLOM

Background

This gene encodes a phosphorylated protein that is a member of a Skp1-Cullin-F-box-like complex. The protein is essential for normal development of the vasculature and mutations in this gene have been associated with glomuvenous malformations, also called glomangiomas. Alternatively spliced variants that encode different protein isoforms have been described but the full-length nature of only one has been determined. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-GLMN antibody used in IHC (Paraffin sections) (IHC-P). GTX103064

GTX103064 IHC-P Image

GLMN antibody detects GLMN protein at cytoplasm in rat stomach by immunohistochemical analysis.
Sample: Paraffin-embedded rat stomach.
GLMN antibody (GTX103064) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-GLMN antibody used in Western Blot (WB). GTX103064

GTX103064 WB Image

Sample (30 ug of whole cell lysate)
A: NIH-3T3
7.5% SDS PAGE
GTX103064 diluted at 1:10000

Anti-GLMN antibody used in IHC (Paraffin sections) (IHC-P). GTX103064

GTX103064 IHC-P Image

GLMN antibody detects GLMN protein at cytoplasm in mouse duodenum by immunohistochemical analysis.
Sample: Paraffin-embedded mouse duodenum.
GLMN antibody (GTX103064) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-GLMN antibody used in Western Blot (WB). GTX103064

GTX103064 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GLMN antibody (GTX103064) diluted at 1:10000.

Anti-GLMN antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103064

GTX103064 ICC/IF Image

GLMN antibody detects GLMN protein at cytoplasm nucleus by immunofluorescent analysis.
Sample: Jurkat cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: GLMN protein stained by GLMN antibody (GTX103064) diluted at 1:100.
Blue: Hoechst 33342 staining.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169