GeneTex
United States (US)

GLMN antibody

GTX103064_40758_WB_19011506_449.jpg
GTX103064_42620_20161206_IFA_19011506_239.jpg
GTX103064_40758_WB_M_19011506_272.jpg
GTX103064_42620_20170426_IHC-P_M_19011506_297.jpg
GTX103064_42620_20170426_IHC-P_R_19011506_963.jpg
GTX103064_42620_20161027_WB_19011519_705.jpg

Cat No. GTX103064

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:5000-1:20000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

68 kDa. ( Note )

Positive Control

Jurkat , Raji , NIH-3T3

Predict Reactivity

Bovine, Chimpanzee(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.23mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human GLMN. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

FAP antibody, FAP48 antibody, FAP68 antibody, FKBPAP antibody, GLML antibody, GVM antibody, VMGLOM antibody, GLMN antibody, glomulin antibody, FKBP-associated protein antibody, FK506-binding protein-associated protein antibody, "glomulin, FKBP associated protein antibody"

Background

This gene encodes a phosphorylated protein that is a member of a Skp1-Cullin-F-box-like complex. The protein is essential for normal development of the vasculature and mutations in this gene have been associated with glomuvenous malformations, also called glomangiomas. Alternatively spliced variants that encode different protein isoforms have been described but the full-length nature of only one has been determined. [provided by RefSeq]

Database

DATA IMAGES
GTX103064_40758_WB_19011506_449.jpg

GTX103064 WB Image

Sample (30 ug of whole cell lysate)
A: Jurkat
B: Raji
7.5% SDS PAGE
GTX103064 diluted at 1:10000

GTX103064_42620_20161206_IFA_19011506_239.jpg

GTX103064 ICC/IF Image

GLMN antibody detects GLMN protein at cytoplasm nucleus by immunofluorescent analysis.
Sample: Jurkat cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: GLMN protein stained by GLMN antibody (GTX103064) diluted at 1:100.
Blue: Hoechst 33342 staining.

GTX103064_40758_WB_M_19011506_272.jpg

GTX103064 WB Image

Sample (30 ug of whole cell lysate)
A: NIH-3T3
7.5% SDS PAGE
GTX103064 diluted at 1:10000

GTX103064_42620_20170426_IHC-P_M_19011506_297.jpg

GTX103064 IHC-P Image

GLMN antibody detects GLMN protein at cytoplasm in mouse duodenum by immunohistochemical analysis.
Sample: Paraffin-embedded mouse duodenum.
GLMN antibody (GTX103064) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX103064_42620_20170426_IHC-P_R_19011506_963.jpg

GTX103064 IHC-P Image

GLMN antibody detects GLMN protein at cytoplasm in rat stomach by immunohistochemical analysis.
Sample: Paraffin-embedded rat stomach.
GLMN antibody (GTX103064) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX103064_42620_20161027_WB_19011519_705.jpg

GTX103064 WB Image

Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GLMN antibody (GTX103064) diluted at 1:10000.

SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169