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GLP1 antibody [10] (Biotin)

Anti-GLP1 antibody [10] (Biotin) used in ELISA (ELISA). GTX60952-02
Anti-GLP1 antibody [10] (Biotin) used in ELISA (ELISA). GTX60952-02

Cat. No. GTX60952-02

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 10

Isotype

 IgG2a

Application

 ELISA

Reactivity

 Human
Package
50 μl ($799)
See all GLP1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
ELISA Assay dependent
Not tested in other applications.

Calculated MW

21 kDa. ( Note )

Product Note

This antibody binds the free N-terminus of GLP-1(7-37) and GLP-1(7-36)amide and shows <0.2% cross-reactivity with GLP-1(9-36)amide, glucagon and human GIP. Cross-reacts 0.25% with GLP-1(1-37), approximately 6% with human GLP-2 and approximately 50% with exendin-4. this antibody cross-reacts with Liraglutide.

PROPERTIES

Form

Liquid

Buffer

0.01M Phosphate buffer pH7.4, 0.14M NaCl

Preservative

15mM Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

Synthetic GLP-1(7-17)

Purification

Biotinylated

Conjugation

Biotin

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

glucagon , GLP-1 , GLP1 , GLP2 , GRPP

Cellular Localization

Secreted

Background

The protein encoded by this gene is actually a preproprotein that is cleaved into four distinct mature peptides. One of these, glucagon, is a pancreatic hormone that counteracts the glucose-lowering action of insulin by stimulating glycogenolysis and gluconeogenesis. Glucagon is a ligand for a specific G-protein linked receptor whose signalling pathway controls cell proliferation. Two of the other peptides are secreted from gut endocrine cells and promote nutrient absorption through distinct mechanisms. Finally, the fourth peptide is similar to glicentin, an active enteroglucagon. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-GLP1 antibody [10] (Biotin) used in ELISA (ELISA). GTX60952-02

GTX60952-02 ELISA Image

The calibration curve of a sandwich assay for Non-amidated GLP-1 using GTX60954 as the capture antibody and GTX60952 -02 as the biotinylated detection antibody.

Anti-GLP1 antibody [10] (Biotin) used in ELISA (ELISA). GTX60952-02

GTX60952-02 ELISA Image

This uses GTX60951 as capture antibody in combination with biotinylated detection antibody GTX60952 -02 exclusively to measure GLP-1(7-36)amide in biological samples. GLP-1(7-36)amide is the principal active form of the peptide, and has been estimated to make up about 70% of the physiological response. Relative to measuring both active forms of GLP-1 together, the responses are reduced by not measuring the approximately 30% contribution of GLP-1(7-37) to the overall response. There is no cross-reactivity with any extended or truncated physiological form of GLP-1(7-36)amide. Specimens must be collected into tubes containing an adequate amount of DPP-4 inhibitor (in addition to routine peptidase inhibitors) to prevent the rapid in vitro degradation of the active peptide.

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 799